Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays
Canine leishmaniasis caused by Leishmania chagasi (L. infantum) is found throughout the South American continent, including Brazil, and dogs are considered to be the main reservoir host for this parasite. To support the implementation of a diagnostic protocol for surveillance of the disease in the r...
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| Veröffentlicht in: | Veterinary research communications 2006-07, Vol.30 (6), p.637-643 |
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| creator | Silva, E.S. da Meide, W.F. van der Schoone, G.J Gontijo, C.M.F Schallig, H.D.F.H Brazil, R.P |
| description | Canine leishmaniasis caused by Leishmania chagasi (L. infantum) is found throughout the South American continent, including Brazil, and dogs are considered to be the main reservoir host for this parasite. To support the implementation of a diagnostic protocol for surveillance of the disease in the region of Belo Horizonte (Minas Gerais, Brazil) we have compared the sensitivity and specificity of two serological tests, indirect immunofluorescent antibody test (IFAT) and direct agglutination test (DAT), with the combination of direct microscopy-culture-PCR as the gold standard, using samples obtained from 103 dogs in the city of Belo Horizonte, Minas Gerais. The currently used standard serodiagnostic test, IFAT, had a sensitivity of 100% and its specificity was 74% compared to the gold standard of the study. The sensitivity and specificity of the DAT were 100% and 91%, respectively. On the basis of this study it is recommended to change from the IFAT to DAT for the serodiagnosis of canine leishmaniasis because of the superior specificity of the test combined with its user-friendliness. |
| doi_str_mv | 10.1007/s11259-006-3324-2 |
| format | Article |
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To support the implementation of a diagnostic protocol for surveillance of the disease in the region of Belo Horizonte (Minas Gerais, Brazil) we have compared the sensitivity and specificity of two serological tests, indirect immunofluorescent antibody test (IFAT) and direct agglutination test (DAT), with the combination of direct microscopy-culture-PCR as the gold standard, using samples obtained from 103 dogs in the city of Belo Horizonte, Minas Gerais. The currently used standard serodiagnostic test, IFAT, had a sensitivity of 100% and its specificity was 74% compared to the gold standard of the study. The sensitivity and specificity of the DAT were 100% and 91%, respectively. On the basis of this study it is recommended to change from the IFAT to DAT for the serodiagnosis of canine leishmaniasis because of the superior specificity of the test combined with its user-friendliness.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-006-3324-2</identifier><identifier>PMID: 16838205</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>agglutination tests ; Agglutination Tests - methods ; Agglutination Tests - veterinary ; Animals ; Brazil ; Diagnosis, Differential ; diagnostic techniques ; disease reservoirs ; Disease Reservoirs - veterinary ; disease surveillance ; DNA, Protozoan - chemistry ; dog diseases ; Dog Diseases - diagnosis ; Dog Diseases - epidemiology ; Dogs ; endemic diseases ; fluorescent antibody technique ; Fluorescent Antibody Technique, Indirect - methods ; Fluorescent Antibody Technique, Indirect - veterinary ; Leishmania chagasi ; Leishmania donovani ; Leishmania infantum ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - epidemiology ; Leishmaniasis, Visceral - veterinary ; pets ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Sensitivity and Specificity ; serodiagnosis ; Seroepidemiologic Studies ; Serologic Tests - methods ; Serologic Tests - veterinary ; test sensitivity ; test specificity ; visceral leishmaniasis</subject><ispartof>Veterinary research communications, 2006-07, Vol.30 (6), p.637-643</ispartof><rights>Springer Science + Business Media, Inc. 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-483c2ac35768ca8207e5859297d31272eb611124c3e58348ed81f90ba84c6cbb3</citedby><cites>FETCH-LOGICAL-c424t-483c2ac35768ca8207e5859297d31272eb611124c3e58348ed81f90ba84c6cbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16838205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, E.S. da</creatorcontrib><creatorcontrib>Meide, W.F. van der</creatorcontrib><creatorcontrib>Schoone, G.J</creatorcontrib><creatorcontrib>Gontijo, C.M.F</creatorcontrib><creatorcontrib>Schallig, H.D.F.H</creatorcontrib><creatorcontrib>Brazil, R.P</creatorcontrib><title>Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><description>Canine leishmaniasis caused by Leishmania chagasi (L. infantum) is found throughout the South American continent, including Brazil, and dogs are considered to be the main reservoir host for this parasite. To support the implementation of a diagnostic protocol for surveillance of the disease in the region of Belo Horizonte (Minas Gerais, Brazil) we have compared the sensitivity and specificity of two serological tests, indirect immunofluorescent antibody test (IFAT) and direct agglutination test (DAT), with the combination of direct microscopy-culture-PCR as the gold standard, using samples obtained from 103 dogs in the city of Belo Horizonte, Minas Gerais. The currently used standard serodiagnostic test, IFAT, had a sensitivity of 100% and its specificity was 74% compared to the gold standard of the study. The sensitivity and specificity of the DAT were 100% and 91%, respectively. On the basis of this study it is recommended to change from the IFAT to DAT for the serodiagnosis of canine leishmaniasis because of the superior specificity of the test combined with its user-friendliness.</description><subject>agglutination tests</subject><subject>Agglutination Tests - methods</subject><subject>Agglutination Tests - veterinary</subject><subject>Animals</subject><subject>Brazil</subject><subject>Diagnosis, Differential</subject><subject>diagnostic techniques</subject><subject>disease reservoirs</subject><subject>Disease Reservoirs - veterinary</subject><subject>disease surveillance</subject><subject>DNA, Protozoan - chemistry</subject><subject>dog diseases</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - epidemiology</subject><subject>Dogs</subject><subject>endemic diseases</subject><subject>fluorescent antibody technique</subject><subject>Fluorescent Antibody Technique, Indirect - methods</subject><subject>Fluorescent Antibody Technique, Indirect - veterinary</subject><subject>Leishmania chagasi</subject><subject>Leishmania donovani</subject><subject>Leishmania infantum</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Leishmaniasis, Visceral - epidemiology</subject><subject>Leishmaniasis, Visceral - veterinary</subject><subject>pets</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Sensitivity and Specificity</subject><subject>serodiagnosis</subject><subject>Seroepidemiologic Studies</subject><subject>Serologic Tests - methods</subject><subject>Serologic Tests - veterinary</subject><subject>test sensitivity</subject><subject>test specificity</subject><subject>visceral leishmaniasis</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3ABiwOnBvwV2zmmu_1AWj4k6NlynEnrKrGLnT1sfwM_uo52JSQunGY0euaVZh6E3lLyiRKiPmdKWd1UhMiKcyYq9gytaK14pYSQz9GKUFlXimtygl7lfE8IaTThL9EJlZprRuoV-rPx9jbE7DOOA17b4APgLfh8N5XeLnMf8HwH-CL0MHmH2wR2Yc9hjPg6Jv8Ywwxn-KsPNuMrSNbnM3ye7KMfcbfHP2wqMQvRhtl3sd9jG3q8-dbiDczgZh8DbnO2-_wavRjsmOHNsZ6im8uLX-vravv96su63VZOMDFXQnPHrOO1ktrZcoeCWtcNa1TPKVMMOknLZ4TjZc6Fhl7ToSGd1cJJ13X8FH085D6k-HsHeTaTzw7G0QaIu2yklkzWSv8XpE1NNZekgB_-Ae_jLoVyhGFKaE2UUAWiB8ilmHOCwTwkP9m0N5SYRag5CDVFqFmEGlZ23h2Dd90E_d-No8ECvD8Ag43G3iafzc1PRign5QdENZo_Ac6rosA</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Silva, E.S. da</creator><creator>Meide, W.F. van der</creator><creator>Schoone, G.J</creator><creator>Gontijo, C.M.F</creator><creator>Schallig, H.D.F.H</creator><creator>Brazil, R.P</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays</title><author>Silva, E.S. da ; Meide, W.F. van der ; Schoone, G.J ; Gontijo, C.M.F ; Schallig, H.D.F.H ; Brazil, R.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-483c2ac35768ca8207e5859297d31272eb611124c3e58348ed81f90ba84c6cbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>agglutination tests</topic><topic>Agglutination Tests - 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Academic</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, E.S. da</au><au>Meide, W.F. van der</au><au>Schoone, G.J</au><au>Gontijo, C.M.F</au><au>Schallig, H.D.F.H</au><au>Brazil, R.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays</atitle><jtitle>Veterinary research communications</jtitle><addtitle>Vet Res Commun</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>30</volume><issue>6</issue><spage>637</spage><epage>643</epage><pages>637-643</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Canine leishmaniasis caused by Leishmania chagasi (L. infantum) is found throughout the South American continent, including Brazil, and dogs are considered to be the main reservoir host for this parasite. To support the implementation of a diagnostic protocol for surveillance of the disease in the region of Belo Horizonte (Minas Gerais, Brazil) we have compared the sensitivity and specificity of two serological tests, indirect immunofluorescent antibody test (IFAT) and direct agglutination test (DAT), with the combination of direct microscopy-culture-PCR as the gold standard, using samples obtained from 103 dogs in the city of Belo Horizonte, Minas Gerais. The currently used standard serodiagnostic test, IFAT, had a sensitivity of 100% and its specificity was 74% compared to the gold standard of the study. The sensitivity and specificity of the DAT were 100% and 91%, respectively. 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| ispartof | Veterinary research communications, 2006-07, Vol.30 (6), p.637-643 |
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| subjects | agglutination tests Agglutination Tests - methods Agglutination Tests - veterinary Animals Brazil Diagnosis, Differential diagnostic techniques disease reservoirs Disease Reservoirs - veterinary disease surveillance DNA, Protozoan - chemistry dog diseases Dog Diseases - diagnosis Dog Diseases - epidemiology Dogs endemic diseases fluorescent antibody technique Fluorescent Antibody Technique, Indirect - methods Fluorescent Antibody Technique, Indirect - veterinary Leishmania chagasi Leishmania donovani Leishmania infantum Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - epidemiology Leishmaniasis, Visceral - veterinary pets polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Sensitivity and Specificity serodiagnosis Seroepidemiologic Studies Serologic Tests - methods Serologic Tests - veterinary test sensitivity test specificity visceral leishmaniasis |
| title | Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays |
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