Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM

The distribution of EP3 receptors on a living cell surface was quantitatively studied by atomic force microscopy (AFM). Green fluorescent protein (GFP) was introduced to the extracellular region of the EP3 receptor on a CHO cell. A microbead was used as a probe to ensure certain contact area, whose...

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Veröffentlicht in:Ultramicroscopy 2006-06, Vol.106 (8), p.652-662
Hauptverfasser: Kim, Hyonchol, Arakawa, Hideo, Hatae, Noriyuki, Sugimoto, Yukihiko, Matsumoto, Osamu, Osada, Toshiya, Ichikawa, Atsushi, Ikai, Atsushi
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Sprache:eng
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