Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation
Free proline accumulation is an innate response of many plants to osmotic stress. To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of delta1-pyrroline-5-carboxylate dehydrogenase (...
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| Veröffentlicht in: | Planta 2005-09, Vol.222 (1), p.70-79 |
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| creator | Miller, G Stein, H Honig, A Kapulnik, Y Zilberstein, A |
| description | Free proline accumulation is an innate response of many plants to osmotic stress. To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of delta1-pyrroline-5-carboxylate dehydrogenase (MsP5CDH) gene were identified and cloned. The two MsPDH genes share a high nucleotide sequence homology and a similar exon/intron structure. Estimation of transcript levels during salt stress and recovery revealed that proline accumulation during stress was linearly correlated with a strong decline in MsPDH transcript levels, while delta1-pyrroline-5-carboxylate synthetase (MsP5CS) and MsP5CDH steady-state transcript levels remained essentially unchanged. MsPDH transcript levels dramatically decreased in a fast, salt concentration-dependent manner. The extent of salt-induced proline accumulation also correlated with salt concentrations. Salt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due to less transcription initiation. Contrary to the salt-dependent repression, a rapid induction of MsPDH transcription occurred at a very early stage of the recovery process, independently of earlier salt treatments. Hence our results suggest the existence of two different regulatory modes of MsPDH expression; the repressing mode that quantifies salt concentration in an as yet unknown mechanism and the "rehydration"-enhancing mode that responds to stress relief in a maximal induction of MsPDH transcription. As yet the components of salt sensing as well as those that might interact with MsPDH promoter to reduce transcription are still unknown. |
| doi_str_mv | 10.1007/s00425-005-1518-4 |
| format | Article |
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To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of delta1-pyrroline-5-carboxylate dehydrogenase (MsP5CDH) gene were identified and cloned. The two MsPDH genes share a high nucleotide sequence homology and a similar exon/intron structure. Estimation of transcript levels during salt stress and recovery revealed that proline accumulation during stress was linearly correlated with a strong decline in MsPDH transcript levels, while delta1-pyrroline-5-carboxylate synthetase (MsP5CS) and MsP5CDH steady-state transcript levels remained essentially unchanged. MsPDH transcript levels dramatically decreased in a fast, salt concentration-dependent manner. The extent of salt-induced proline accumulation also correlated with salt concentrations. Salt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due to less transcription initiation. Contrary to the salt-dependent repression, a rapid induction of MsPDH transcription occurred at a very early stage of the recovery process, independently of earlier salt treatments. Hence our results suggest the existence of two different regulatory modes of MsPDH expression; the repressing mode that quantifies salt concentration in an as yet unknown mechanism and the "rehydration"-enhancing mode that responds to stress relief in a maximal induction of MsPDH transcription. As yet the components of salt sensing as well as those that might interact with MsPDH promoter to reduce transcription are still unknown.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-005-1518-4</identifier><identifier>PMID: 15809861</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>abscisic acid ; Abscisic Acid - pharmacology ; Accumulation ; Alfalfa ; amine and amino acid oxidoreductases ; Bacteria ; beta-glucuronidase ; Biological and medical sciences ; Complementary DNA ; Dehydrogenase ; Dehydrogenases ; delta-pyrroline-5-carboxylate dehydrogenase ; DNA, Complementary - genetics ; Enzymes ; forage crops ; free amino acids ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Plant - drug effects ; Genes ; Genes, Plant - genetics ; Leaves ; Medicago sativa ; Medicago sativa - drug effects ; Medicago sativa - enzymology ; Medicago sativa - genetics ; Medicago sativa - metabolism ; messenger RNA ; Molecular Sequence Data ; nucleotide sequences ; plant biochemistry ; plant genetics ; Plant Leaves - enzymology ; Plant physiology and development ; plant proteins ; Plant Roots - enzymology ; Plants ; proline ; Proline - metabolism ; proline dehydrogenase ; Proline Oxidase - genetics ; Promoter regions ; Promoter Regions, Genetic - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Plant - genetics ; RNA, Plant - metabolism ; salt stress ; Salts ; signal transduction ; Sodium Chloride - pharmacology ; Stress ; Table salt ; transcription (genetics) ; Transcription, Genetic - genetics ; Water and solutes. Absorption, translocation and permeability</subject><ispartof>Planta, 2005-09, Vol.222 (1), p.70-79</ispartof><rights>Springer-Verlag Berlin Heidelberg 2005</rights><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-78fc6e658f05a0209b9314daac9a92e23af01853b0ac0b8f34252d2160d295d3</citedby><cites>FETCH-LOGICAL-c402t-78fc6e658f05a0209b9314daac9a92e23af01853b0ac0b8f34252d2160d295d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23388961$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23388961$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17104554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15809861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, G</creatorcontrib><creatorcontrib>Stein, H</creatorcontrib><creatorcontrib>Honig, A</creatorcontrib><creatorcontrib>Kapulnik, Y</creatorcontrib><creatorcontrib>Zilberstein, A</creatorcontrib><title>Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation</title><title>Planta</title><addtitle>Planta</addtitle><description>Free proline accumulation is an innate response of many plants to osmotic stress. To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of delta1-pyrroline-5-carboxylate dehydrogenase (MsP5CDH) gene were identified and cloned. The two MsPDH genes share a high nucleotide sequence homology and a similar exon/intron structure. Estimation of transcript levels during salt stress and recovery revealed that proline accumulation during stress was linearly correlated with a strong decline in MsPDH transcript levels, while delta1-pyrroline-5-carboxylate synthetase (MsP5CS) and MsP5CDH steady-state transcript levels remained essentially unchanged. MsPDH transcript levels dramatically decreased in a fast, salt concentration-dependent manner. The extent of salt-induced proline accumulation also correlated with salt concentrations. Salt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due to less transcription initiation. Contrary to the salt-dependent repression, a rapid induction of MsPDH transcription occurred at a very early stage of the recovery process, independently of earlier salt treatments. Hence our results suggest the existence of two different regulatory modes of MsPDH expression; the repressing mode that quantifies salt concentration in an as yet unknown mechanism and the "rehydration"-enhancing mode that responds to stress relief in a maximal induction of MsPDH transcription. As yet the components of salt sensing as well as those that might interact with MsPDH promoter to reduce transcription are still unknown.</description><subject>abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Accumulation</subject><subject>Alfalfa</subject><subject>amine and amino acid oxidoreductases</subject><subject>Bacteria</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Complementary DNA</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>delta-pyrroline-5-carboxylate dehydrogenase</subject><subject>DNA, Complementary - genetics</subject><subject>Enzymes</subject><subject>forage crops</subject><subject>free amino acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Leaves</subject><subject>Medicago sativa</subject><subject>Medicago sativa - drug effects</subject><subject>Medicago sativa - enzymology</subject><subject>Medicago sativa - genetics</subject><subject>Medicago sativa - metabolism</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>plant biochemistry</subject><subject>plant genetics</subject><subject>Plant Leaves - enzymology</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plant Roots - enzymology</subject><subject>Plants</subject><subject>proline</subject><subject>Proline - metabolism</subject><subject>proline dehydrogenase</subject><subject>Proline Oxidase - genetics</subject><subject>Promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>salt stress</subject><subject>Salts</subject><subject>signal transduction</subject><subject>Sodium Chloride - pharmacology</subject><subject>Stress</subject><subject>Table salt</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic - genetics</subject><subject>Water and solutes. Absorption, translocation and permeability</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkU2LFDEQhoMo7rj6AzyoQdBbayXpdKePy-IXrAi6nkNNUhl76OmMSffAHP3nZuxhFzxV4H3qocjL2HMB7wRA-z4D1FJXALoSWpiqfsBWolayklCbh2wFUN7QKX3BnuS8BShh2z5mF0Ib6EwjVuzPd8r7OOb-QHwXPWUeA_9Kvne4iTzj1B-Q71Mc-pG4p19Hn-KGRszEyyi4n1M_bgo5TDxPiXLmOHqeyMUDpSMvpgkn4iER3YnQuXk3D8Uex6fsUcAh07PzvGS3Hz_cXn-ubr59-nJ9dVO5GuRUtSa4hhptAmgECd26U6L2iK7DTpJUGEAYrdaADtYmqPIz0kvRgJed9uqSvV205YbfM-XJ7vrsaBhwpDhn25hGKl2LAr7-D9zGOY3lNGskGANSdgUSC-RSzDlRsPvU7zAdrQB76sYu3djSjT11Y-uy8_Isntc78vcb5zIK8OYMYHY4hISj6_M91wqotT6JXizcNk8x3eVSKWO6f55XSx4wWtyk4vj5Q4JQIKBpQLbqL6uwqx8</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Miller, G</creator><creator>Stein, H</creator><creator>Honig, A</creator><creator>Kapulnik, Y</creator><creator>Zilberstein, A</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation</title><author>Miller, G ; Stein, H ; Honig, A ; Kapulnik, Y ; Zilberstein, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-78fc6e658f05a0209b9314daac9a92e23af01853b0ac0b8f34252d2160d295d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Accumulation</topic><topic>Alfalfa</topic><topic>amine and amino acid oxidoreductases</topic><topic>Bacteria</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Complementary DNA</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>delta-pyrroline-5-carboxylate dehydrogenase</topic><topic>DNA, Complementary - genetics</topic><topic>Enzymes</topic><topic>forage crops</topic><topic>free amino acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes</topic><topic>Genes, Plant - genetics</topic><topic>Leaves</topic><topic>Medicago sativa</topic><topic>Medicago sativa - drug effects</topic><topic>Medicago sativa - enzymology</topic><topic>Medicago sativa - genetics</topic><topic>Medicago sativa - metabolism</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>plant biochemistry</topic><topic>plant genetics</topic><topic>Plant Leaves - enzymology</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plant Roots - enzymology</topic><topic>Plants</topic><topic>proline</topic><topic>Proline - metabolism</topic><topic>proline dehydrogenase</topic><topic>Proline Oxidase - genetics</topic><topic>Promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>salt stress</topic><topic>Salts</topic><topic>signal transduction</topic><topic>Sodium Chloride - pharmacology</topic><topic>Stress</topic><topic>Table salt</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic - genetics</topic><topic>Water and solutes. Absorption, translocation and permeability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, G</creatorcontrib><creatorcontrib>Stein, H</creatorcontrib><creatorcontrib>Honig, A</creatorcontrib><creatorcontrib>Kapulnik, Y</creatorcontrib><creatorcontrib>Zilberstein, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, G</au><au>Stein, H</au><au>Honig, A</au><au>Kapulnik, Y</au><au>Zilberstein, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>222</volume><issue>1</issue><spage>70</spage><epage>79</epage><pages>70-79</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Free proline accumulation is an innate response of many plants to osmotic stress. To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of delta1-pyrroline-5-carboxylate dehydrogenase (MsP5CDH) gene were identified and cloned. The two MsPDH genes share a high nucleotide sequence homology and a similar exon/intron structure. Estimation of transcript levels during salt stress and recovery revealed that proline accumulation during stress was linearly correlated with a strong decline in MsPDH transcript levels, while delta1-pyrroline-5-carboxylate synthetase (MsP5CS) and MsP5CDH steady-state transcript levels remained essentially unchanged. MsPDH transcript levels dramatically decreased in a fast, salt concentration-dependent manner. The extent of salt-induced proline accumulation also correlated with salt concentrations. Salt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due to less transcription initiation. Contrary to the salt-dependent repression, a rapid induction of MsPDH transcription occurred at a very early stage of the recovery process, independently of earlier salt treatments. Hence our results suggest the existence of two different regulatory modes of MsPDH expression; the repressing mode that quantifies salt concentration in an as yet unknown mechanism and the "rehydration"-enhancing mode that responds to stress relief in a maximal induction of MsPDH transcription. As yet the components of salt sensing as well as those that might interact with MsPDH promoter to reduce transcription are still unknown.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>15809861</pmid><doi>10.1007/s00425-005-1518-4</doi><tpages>10</tpages></addata></record> |
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| issn | 0032-0935 1432-2048 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals |
| subjects | abscisic acid Abscisic Acid - pharmacology Accumulation Alfalfa amine and amino acid oxidoreductases Bacteria beta-glucuronidase Biological and medical sciences Complementary DNA Dehydrogenase Dehydrogenases delta-pyrroline-5-carboxylate dehydrogenase DNA, Complementary - genetics Enzymes forage crops free amino acids Fundamental and applied biological sciences. Psychology Gene expression regulation Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Plant - drug effects Genes Genes, Plant - genetics Leaves Medicago sativa Medicago sativa - drug effects Medicago sativa - enzymology Medicago sativa - genetics Medicago sativa - metabolism messenger RNA Molecular Sequence Data nucleotide sequences plant biochemistry plant genetics Plant Leaves - enzymology Plant physiology and development plant proteins Plant Roots - enzymology Plants proline Proline - metabolism proline dehydrogenase Proline Oxidase - genetics Promoter regions Promoter Regions, Genetic - genetics RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant - genetics RNA, Plant - metabolism salt stress Salts signal transduction Sodium Chloride - pharmacology Stress Table salt transcription (genetics) Transcription, Genetic - genetics Water and solutes. Absorption, translocation and permeability |
| title | Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation |
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