Spatial and Temporal Localization of FGF-2 and VEGF in Healing Tooth Extraction Sockets in a Rabbit Model
The purpose of this study was to test the hypothesis that spatial and temporal localization of growth factors FGF-2 and VEGF in a rabbit tooth extraction socket model correlate with the histologic events of healing. Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in t...
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| Veröffentlicht in: | Journal of oral and maxillofacial surgery 2005-10, Vol.63 (10), p.1500-1508 |
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| creator | Lalani, Zahid Wong, Mark Brey, Eric M. Mikos, Antonios G. Duke, P.J. Miller, Michael J. Johnston, Carol Montufar-Solis, Dina |
| description | The purpose of this study was to test the hypothesis that spatial and temporal localization of growth factors FGF-2 and VEGF in a rabbit tooth extraction socket model correlate with the histologic events of healing.
Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in the study. Incisor teeth were extracted from both jaws and the healing extraction socket with surrounding jaw bone was harvested at 48 hours, 4 days, 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin-eosin and immunohistochemical staining. The sections were stained to detect FGF-2 and VEGF. The stained sections were then imaged and an automated computer program was used to detect the brown diaminobenzidine stain that represented the growth factors of interest. Data was obtained in the form of percentage area and intensity of stain and analyzed using the analysis of variance (ANOVA - Tukey Kramer and Scheffe’s post-test).
Spatial and temporal differences in localization of FGF-2 and VEGF were observed across all time frames in both jaws. Statistically significant differences in percentage area and intensity of brown diaminobenzidine stain were seen temporally between FGF-2 and VEGF (
P < .05).
The results of this study showed positive correlation of histologic events to spatial and temporal localization of FGF-2 and VEGF in a rabbit tooth extraction model. |
| doi_str_mv | 10.1016/j.joms.2005.03.032 |
| format | Article |
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Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in the study. Incisor teeth were extracted from both jaws and the healing extraction socket with surrounding jaw bone was harvested at 48 hours, 4 days, 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin-eosin and immunohistochemical staining. The sections were stained to detect FGF-2 and VEGF. The stained sections were then imaged and an automated computer program was used to detect the brown diaminobenzidine stain that represented the growth factors of interest. Data was obtained in the form of percentage area and intensity of stain and analyzed using the analysis of variance (ANOVA - Tukey Kramer and Scheffe’s post-test).
Spatial and temporal differences in localization of FGF-2 and VEGF were observed across all time frames in both jaws. Statistically significant differences in percentage area and intensity of brown diaminobenzidine stain were seen temporally between FGF-2 and VEGF (
P < .05).
The results of this study showed positive correlation of histologic events to spatial and temporal localization of FGF-2 and VEGF in a rabbit tooth extraction model.</description><identifier>ISSN: 0278-2391</identifier><identifier>EISSN: 1531-5053</identifier><identifier>DOI: 10.1016/j.joms.2005.03.032</identifier><identifier>PMID: 16182919</identifier><identifier>CODEN: JOMSDA</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Analysis of Variance ; Animals ; Biological and medical sciences ; Bone Regeneration - physiology ; Dentistry ; Extracellular Matrix - chemistry ; Extracellular Matrix - metabolism ; Fibroblast Growth Factor 2 - analysis ; Fibroblast Growth Factor 2 - metabolism ; Immunohistochemistry ; Male ; Medical sciences ; Models, Animal ; Otorhinolaryngology. Stomatology ; Rabbits ; Statistics, Nonparametric ; Tissue Distribution ; Tooth Extraction ; Tooth Socket - chemistry ; Tooth Socket - metabolism ; Tooth Socket - physiology ; Vascular Endothelial Growth Factor A - analysis ; Vascular Endothelial Growth Factor A - metabolism ; Wound Healing - physiology</subject><ispartof>Journal of oral and maxillofacial surgery, 2005-10, Vol.63 (10), p.1500-1508</ispartof><rights>2005 American Association of Oral and Maxillofacial Surgeons</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-ff977aa38494b2e53a97cbcee29688001a1e9382c93680bdf2de02f7f37412c33</citedby><cites>FETCH-LOGICAL-c481t-ff977aa38494b2e53a97cbcee29688001a1e9382c93680bdf2de02f7f37412c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joms.2005.03.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17155125$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16182919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lalani, Zahid</creatorcontrib><creatorcontrib>Wong, Mark</creatorcontrib><creatorcontrib>Brey, Eric M.</creatorcontrib><creatorcontrib>Mikos, Antonios G.</creatorcontrib><creatorcontrib>Duke, P.J.</creatorcontrib><creatorcontrib>Miller, Michael J.</creatorcontrib><creatorcontrib>Johnston, Carol</creatorcontrib><creatorcontrib>Montufar-Solis, Dina</creatorcontrib><title>Spatial and Temporal Localization of FGF-2 and VEGF in Healing Tooth Extraction Sockets in a Rabbit Model</title><title>Journal of oral and maxillofacial surgery</title><addtitle>J Oral Maxillofac Surg</addtitle><description>The purpose of this study was to test the hypothesis that spatial and temporal localization of growth factors FGF-2 and VEGF in a rabbit tooth extraction socket model correlate with the histologic events of healing.
Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in the study. Incisor teeth were extracted from both jaws and the healing extraction socket with surrounding jaw bone was harvested at 48 hours, 4 days, 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin-eosin and immunohistochemical staining. The sections were stained to detect FGF-2 and VEGF. The stained sections were then imaged and an automated computer program was used to detect the brown diaminobenzidine stain that represented the growth factors of interest. Data was obtained in the form of percentage area and intensity of stain and analyzed using the analysis of variance (ANOVA - Tukey Kramer and Scheffe’s post-test).
Spatial and temporal differences in localization of FGF-2 and VEGF were observed across all time frames in both jaws. Statistically significant differences in percentage area and intensity of brown diaminobenzidine stain were seen temporally between FGF-2 and VEGF (
P < .05).
The results of this study showed positive correlation of histologic events to spatial and temporal localization of FGF-2 and VEGF in a rabbit tooth extraction model.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Regeneration - physiology</subject><subject>Dentistry</subject><subject>Extracellular Matrix - chemistry</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblast Growth Factor 2 - analysis</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Models, Animal</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Rabbits</subject><subject>Statistics, Nonparametric</subject><subject>Tissue Distribution</subject><subject>Tooth Extraction</subject><subject>Tooth Socket - chemistry</subject><subject>Tooth Socket - metabolism</subject><subject>Tooth Socket - physiology</subject><subject>Vascular Endothelial Growth Factor A - analysis</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Wound Healing - physiology</subject><issn>0278-2391</issn><issn>1531-5053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0V-LEzEQAPAgildPv4APkhfvbWv-bDYJ-CJH2xMqgld9DdnsRFN3NzXZinef3uy1cG8KAyHkN8NkBqHXlCwpoc27_XIfh7xkhIgl4SXYE7SggtNKEMGfogVhUlWMa3qBXuS8J4RSIZvn6II2VDFN9QKF24Odgu2xHTu8g-EQU7lso7N9uC8vccTR4_VmXbEH8m21WeMw4hsoYPyOdzFOP_Dqz5Sse9C30f2EKc_G4i-2bcOEP8UO-pfombd9hlfn8xJ9Xa921zfV9vPm4_WHbeVqRafKey2ltVzVum4ZCG61dK0DYLpRqnzBUtBcMad5o0jbedYBYV56LmvKHOeX6OpU95DiryPkyQwhO-h7O0I8ZtOohlEh6H8hlTVRRMgC2Qm6FHNO4M0hhcGmO0OJmTdh9mbehJk3YQgvwUrSm3P1YztA95hyHn0Bb8_A5jJtn-zoQn50cm6SieLenxyUof0OkEx2AUYHXUjgJtPF8K8-_gIdxqUJ</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Lalani, Zahid</creator><creator>Wong, Mark</creator><creator>Brey, Eric M.</creator><creator>Mikos, Antonios G.</creator><creator>Duke, P.J.</creator><creator>Miller, Michael J.</creator><creator>Johnston, Carol</creator><creator>Montufar-Solis, Dina</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>20051001</creationdate><title>Spatial and Temporal Localization of FGF-2 and VEGF in Healing Tooth Extraction Sockets in a Rabbit Model</title><author>Lalani, Zahid ; Wong, Mark ; Brey, Eric M. ; Mikos, Antonios G. ; Duke, P.J. ; Miller, Michael J. ; Johnston, Carol ; Montufar-Solis, Dina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-ff977aa38494b2e53a97cbcee29688001a1e9382c93680bdf2de02f7f37412c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Regeneration - physiology</topic><topic>Dentistry</topic><topic>Extracellular Matrix - chemistry</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblast Growth Factor 2 - analysis</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Rabbits</topic><topic>Statistics, Nonparametric</topic><topic>Tissue Distribution</topic><topic>Tooth Extraction</topic><topic>Tooth Socket - chemistry</topic><topic>Tooth Socket - metabolism</topic><topic>Tooth Socket - physiology</topic><topic>Vascular Endothelial Growth Factor A - analysis</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lalani, Zahid</creatorcontrib><creatorcontrib>Wong, Mark</creatorcontrib><creatorcontrib>Brey, Eric M.</creatorcontrib><creatorcontrib>Mikos, Antonios G.</creatorcontrib><creatorcontrib>Duke, P.J.</creatorcontrib><creatorcontrib>Miller, Michael J.</creatorcontrib><creatorcontrib>Johnston, Carol</creatorcontrib><creatorcontrib>Montufar-Solis, Dina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Journal of oral and maxillofacial surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lalani, Zahid</au><au>Wong, Mark</au><au>Brey, Eric M.</au><au>Mikos, Antonios G.</au><au>Duke, P.J.</au><au>Miller, Michael J.</au><au>Johnston, Carol</au><au>Montufar-Solis, Dina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial and Temporal Localization of FGF-2 and VEGF in Healing Tooth Extraction Sockets in a Rabbit Model</atitle><jtitle>Journal of oral and maxillofacial surgery</jtitle><addtitle>J Oral Maxillofac Surg</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>63</volume><issue>10</issue><spage>1500</spage><epage>1508</epage><pages>1500-1508</pages><issn>0278-2391</issn><eissn>1531-5053</eissn><coden>JOMSDA</coden><abstract>The purpose of this study was to test the hypothesis that spatial and temporal localization of growth factors FGF-2 and VEGF in a rabbit tooth extraction socket model correlate with the histologic events of healing.
Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in the study. Incisor teeth were extracted from both jaws and the healing extraction socket with surrounding jaw bone was harvested at 48 hours, 4 days, 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin-eosin and immunohistochemical staining. The sections were stained to detect FGF-2 and VEGF. The stained sections were then imaged and an automated computer program was used to detect the brown diaminobenzidine stain that represented the growth factors of interest. Data was obtained in the form of percentage area and intensity of stain and analyzed using the analysis of variance (ANOVA - Tukey Kramer and Scheffe’s post-test).
Spatial and temporal differences in localization of FGF-2 and VEGF were observed across all time frames in both jaws. Statistically significant differences in percentage area and intensity of brown diaminobenzidine stain were seen temporally between FGF-2 and VEGF (
P < .05).
The results of this study showed positive correlation of histologic events to spatial and temporal localization of FGF-2 and VEGF in a rabbit tooth extraction model.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16182919</pmid><doi>10.1016/j.joms.2005.03.032</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of oral and maxillofacial surgery, 2005-10, Vol.63 (10), p.1500-1508 |
| issn | 0278-2391 1531-5053 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis of Variance Animals Biological and medical sciences Bone Regeneration - physiology Dentistry Extracellular Matrix - chemistry Extracellular Matrix - metabolism Fibroblast Growth Factor 2 - analysis Fibroblast Growth Factor 2 - metabolism Immunohistochemistry Male Medical sciences Models, Animal Otorhinolaryngology. Stomatology Rabbits Statistics, Nonparametric Tissue Distribution Tooth Extraction Tooth Socket - chemistry Tooth Socket - metabolism Tooth Socket - physiology Vascular Endothelial Growth Factor A - analysis Vascular Endothelial Growth Factor A - metabolism Wound Healing - physiology |
| title | Spatial and Temporal Localization of FGF-2 and VEGF in Healing Tooth Extraction Sockets in a Rabbit Model |
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