Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas
Background: Microsatellite instability (MSI) is an important marker in colorectal cancer. The analysis may be difficult if the tumour is heterogeneous or only scarce material is available. The aim of this study was to apply laser microdissection (LMD) to MSI analysis in an attempt to allow diagnosis...
Gespeichert in:
| Veröffentlicht in: | Anticancer research 2006-05, Vol.26 (3A), p.2069-2074 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2074 |
|---|---|
| container_issue | 3A |
| container_start_page | 2069 |
| container_title | Anticancer research |
| container_volume | 26 |
| creator | JENSEN, Lars Henrik CRUGER, Dorthe G LINDEBJERG, Jan BYRIEL, Lene BRUUN-PETERSEN, Gert JAKOBSEN, Anders |
| description | Background: Microsatellite instability (MSI) is an important marker in colorectal cancer. The analysis may be difficult if
the tumour is heterogeneous or only scarce material is available. The aim of this study was to apply laser microdissection
(LMD) to MSI analysis in an attempt to allow diagnosis in these situations. Materials and Methods: Twenty-two primary tumours
and eight lymph node metastases from twenty patients were laser microdissected and MSI analysis was performed with an optimised
multiplex PCR. Differences in allelic size between tumour and blood were evaluated to determine the MSI status. Results: The
method proved efficient in as little as 4,000 μm 3 formalin-treated and paraffin-embedded tumour tissue. The result of microsatellite analysis was independent of sample location
in the primary tumour and its metastasis. Conclusion: LMD followed by a multiplex PCR is a useful method for MSI analysis
in cases of tumour heterogeneity and scarce tumour material. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68616698</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68616698</sourcerecordid><originalsourceid>FETCH-LOGICAL-h270t-55638fd7d88d56fd03d8417ccefc2db568f6b155c1b149bf02010c14bc3b798f3</originalsourceid><addsrcrecordid>eNpF0ElLAzEUB_Agiq3VryBz0dtAlskyx1LcoNKLnkMmi41kJjVvivjtHWylpwePH_-3nKE5kS2pJWf4HM0x5biWGPMZugL4xFiIVrFLNCNCUUkaMUebtQFfqtdoS3YRwNsx5qEygzv0wIw-pTj6ajmY9AMRqhyqVU65TNSkaun8kK0pNg65N3CNLoJJ4G-OdYHeHx_eVs_1evP0slqu6y2VeKw5F0wFJ51SjovgMHOqIdJaHyx1HRcqiI5wbklHmrYLmGKCLWk6yzrZqsAW6P6Quyv5a-9h1H0EO61qBp_3oIUS5O_aBbo9wn3Xe6d3Jfam_Oj_F0zg7ggMWJNCMYONcHKyxZi27DRxGz-237F4Db1JaYpl2hQqNFtqikXLfgGlY3Na</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68616698</pqid></control><display><type>article</type><title>Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>JENSEN, Lars Henrik ; CRUGER, Dorthe G ; LINDEBJERG, Jan ; BYRIEL, Lene ; BRUUN-PETERSEN, Gert ; JAKOBSEN, Anders</creator><creatorcontrib>JENSEN, Lars Henrik ; CRUGER, Dorthe G ; LINDEBJERG, Jan ; BYRIEL, Lene ; BRUUN-PETERSEN, Gert ; JAKOBSEN, Anders</creatorcontrib><description>Background: Microsatellite instability (MSI) is an important marker in colorectal cancer. The analysis may be difficult if
the tumour is heterogeneous or only scarce material is available. The aim of this study was to apply laser microdissection
(LMD) to MSI analysis in an attempt to allow diagnosis in these situations. Materials and Methods: Twenty-two primary tumours
and eight lymph node metastases from twenty patients were laser microdissected and MSI analysis was performed with an optimised
multiplex PCR. Differences in allelic size between tumour and blood were evaluated to determine the MSI status. Results: The
method proved efficient in as little as 4,000 μm 3 formalin-treated and paraffin-embedded tumour tissue. The result of microsatellite analysis was independent of sample location
in the primary tumour and its metastasis. Conclusion: LMD followed by a multiplex PCR is a useful method for MSI analysis
in cases of tumour heterogeneity and scarce tumour material.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 16827146</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Female ; Formaldehyde ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Lasers ; Male ; Medical sciences ; Microdissection ; Microsatellite Repeats - genetics ; Polymerase Chain Reaction ; Reproducibility of Results ; Sensitivity and Specificity ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumors</subject><ispartof>Anticancer research, 2006-05, Vol.26 (3A), p.2069-2074</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17900293$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16827146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JENSEN, Lars Henrik</creatorcontrib><creatorcontrib>CRUGER, Dorthe G</creatorcontrib><creatorcontrib>LINDEBJERG, Jan</creatorcontrib><creatorcontrib>BYRIEL, Lene</creatorcontrib><creatorcontrib>BRUUN-PETERSEN, Gert</creatorcontrib><creatorcontrib>JAKOBSEN, Anders</creatorcontrib><title>Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Microsatellite instability (MSI) is an important marker in colorectal cancer. The analysis may be difficult if
the tumour is heterogeneous or only scarce material is available. The aim of this study was to apply laser microdissection
(LMD) to MSI analysis in an attempt to allow diagnosis in these situations. Materials and Methods: Twenty-two primary tumours
and eight lymph node metastases from twenty patients were laser microdissected and MSI analysis was performed with an optimised
multiplex PCR. Differences in allelic size between tumour and blood were evaluated to determine the MSI status. Results: The
method proved efficient in as little as 4,000 μm 3 formalin-treated and paraffin-embedded tumour tissue. The result of microsatellite analysis was independent of sample location
in the primary tumour and its metastasis. Conclusion: LMD followed by a multiplex PCR is a useful method for MSI analysis
in cases of tumour heterogeneity and scarce tumour material.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Female</subject><subject>Formaldehyde</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Lasers</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microdissection</subject><subject>Microsatellite Repeats - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0ElLAzEUB_Agiq3VryBz0dtAlskyx1LcoNKLnkMmi41kJjVvivjtHWylpwePH_-3nKE5kS2pJWf4HM0x5biWGPMZugL4xFiIVrFLNCNCUUkaMUebtQFfqtdoS3YRwNsx5qEygzv0wIw-pTj6ajmY9AMRqhyqVU65TNSkaun8kK0pNg65N3CNLoJJ4G-OdYHeHx_eVs_1evP0slqu6y2VeKw5F0wFJ51SjovgMHOqIdJaHyx1HRcqiI5wbklHmrYLmGKCLWk6yzrZqsAW6P6Quyv5a-9h1H0EO61qBp_3oIUS5O_aBbo9wn3Xe6d3Jfam_Oj_F0zg7ggMWJNCMYONcHKyxZi27DRxGz-237F4Db1JaYpl2hQqNFtqikXLfgGlY3Na</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>JENSEN, Lars Henrik</creator><creator>CRUGER, Dorthe G</creator><creator>LINDEBJERG, Jan</creator><creator>BYRIEL, Lene</creator><creator>BRUUN-PETERSEN, Gert</creator><creator>JAKOBSEN, Anders</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas</title><author>JENSEN, Lars Henrik ; CRUGER, Dorthe G ; LINDEBJERG, Jan ; BYRIEL, Lene ; BRUUN-PETERSEN, Gert ; JAKOBSEN, Anders</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-55638fd7d88d56fd03d8417ccefc2db568f6b155c1b149bf02010c14bc3b798f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - pathology</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Female</topic><topic>Formaldehyde</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Lasers</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microdissection</topic><topic>Microsatellite Repeats - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JENSEN, Lars Henrik</creatorcontrib><creatorcontrib>CRUGER, Dorthe G</creatorcontrib><creatorcontrib>LINDEBJERG, Jan</creatorcontrib><creatorcontrib>BYRIEL, Lene</creatorcontrib><creatorcontrib>BRUUN-PETERSEN, Gert</creatorcontrib><creatorcontrib>JAKOBSEN, Anders</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JENSEN, Lars Henrik</au><au>CRUGER, Dorthe G</au><au>LINDEBJERG, Jan</au><au>BYRIEL, Lene</au><au>BRUUN-PETERSEN, Gert</au><au>JAKOBSEN, Anders</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>26</volume><issue>3A</issue><spage>2069</spage><epage>2074</epage><pages>2069-2074</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Microsatellite instability (MSI) is an important marker in colorectal cancer. The analysis may be difficult if
the tumour is heterogeneous or only scarce material is available. The aim of this study was to apply laser microdissection
(LMD) to MSI analysis in an attempt to allow diagnosis in these situations. Materials and Methods: Twenty-two primary tumours
and eight lymph node metastases from twenty patients were laser microdissected and MSI analysis was performed with an optimised
multiplex PCR. Differences in allelic size between tumour and blood were evaluated to determine the MSI status. Results: The
method proved efficient in as little as 4,000 μm 3 formalin-treated and paraffin-embedded tumour tissue. The result of microsatellite analysis was independent of sample location
in the primary tumour and its metastasis. Conclusion: LMD followed by a multiplex PCR is a useful method for MSI analysis
in cases of tumour heterogeneity and scarce tumour material.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>16827146</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0250-7005 |
| ispartof | Anticancer research, 2006-05, Vol.26 (3A), p.2069-2074 |
| issn | 0250-7005 1791-7530 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68616698 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenocarcinoma - genetics Adenocarcinoma - pathology Aged Aged, 80 and over Biological and medical sciences Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Female Formaldehyde Gastroenterology. Liver. Pancreas. Abdomen Humans Lasers Male Medical sciences Microdissection Microsatellite Repeats - genetics Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
| title | Laser Microdissection and Microsatellite Analysis of Colorectal Adenocarcinomas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T06%3A20%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Laser%20Microdissection%20and%20Microsatellite%20Analysis%20of%20Colorectal%20Adenocarcinomas&rft.jtitle=Anticancer%20research&rft.au=JENSEN,%20Lars%20Henrik&rft.date=2006-05-01&rft.volume=26&rft.issue=3A&rft.spage=2069&rft.epage=2074&rft.pages=2069-2074&rft.issn=0250-7005&rft.eissn=1791-7530&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E68616698%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68616698&rft_id=info:pmid/16827146&rfr_iscdi=true |