Pitfalls in Detection of Contaminating Neuroblastoma Cells by Tyrosine Hydroxylase RT-PCR Due to Catecholamine-producing Hematopoietic Cells

Background: RT-PCR analysis of compounds of catecholamine metabolism (in particular tyrosine hydroxylase, TH) is widely used for the detection of contaminating neuroblastoma cells in hematopoietic stem cell preparations. Due to reports in the literature showing that hematopoietic cells are also able...

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Veröffentlicht in:	Anticancer research 2006-05, Vol.26 (3A), p.2075-2080
Hauptverfasser:	KUCI, Zyrafete, SEITZ, Gabriele, KUCI, Selim, KREYENBERG, Hermann, SCHUMM, Michael, LANG, Peter, NIETHAMMER, Dietrich, HANDGRETINGER, Rupert, BRUCHELT, Gernot
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Catecholamines - biosynthesis Cell Line, Tumor Dopamine beta-Hydroxylase - analysis Dopamine beta-Hydroxylase - biosynthesis Dopamine beta-Hydroxylase - genetics Gene Expression Profiling Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - enzymology Hematopoietic Stem Cells - metabolism Humans Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - enzymology Leukocytes, Mononuclear - metabolism Medical sciences Neuroblastoma - enzymology Neuroblastoma - genetics Neuroblastoma - pathology Neurology Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Tumors Tumors of the nervous system. Phacomatoses Tyrosine 3-Monooxygenase - analysis Tyrosine 3-Monooxygenase - biosynthesis Tyrosine 3-Monooxygenase - genetics
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