Pumping of mammalian cells with a nozzle-diffuser micropump

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-es...

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Veröffentlicht in:	Lab on a chip 2005-01, Vol.5 (10), p.1083-1088
Hauptverfasser:	Yamahata, Christophe, Vandevyver, Caroline, Lacharme, Frédéric, Izewska, Paulina, Vogel, Horst, Freitag, Ruth, Gijs, Martin A M
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - chemistry Adenosine Triphosphate - metabolism Animals Calcium - metabolism Cell Biology Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line, Tumor Cell Proliferation Cell Survival Culture Techniques Diffusion Equipment Design Humans Hybridomas - cytology Jurkat Cells L-Lactate Dehydrogenase - metabolism Tetrazolium Salts - pharmacology Time Factors Trypan Blue - pharmacology
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