Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI
BACKGROUND: Each year 40 000 men have a vasectomy in the UK whilst another 2400 request a reversal to begin a second family. Sperm can now be obtained by testicular biopsy and subsequently used in assisted conception with ICSI. The study aims were to compare sperm yields of men post-vasectomy or wit...
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| Veröffentlicht in: | Human reproduction (Oxford) 2005-10, Vol.20 (10), p.2795-2800 |
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| creator | McVicar, C.M. O’Neill, D.A. McClure, N. Clements, B. McCullough, S. Lewis, S.E.M. |
| description | BACKGROUND: Each year 40 000 men have a vasectomy in the UK whilst another 2400 request a reversal to begin a second family. Sperm can now be obtained by testicular biopsy and subsequently used in assisted conception with ICSI. The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI. METHODS: Testicular tissue was obtained by Trucut needle from men who had undergone a vasectomy >5 years previously or had OA from other causes and from fertile men during vasectomy. Seminiferous tubules were milked to measure sperm yields. Numbers of Sertoli cells and spermatids and thickness of the seminiferous tubule walls were assessed using quantitative computerized analysis. RESULTS and CONCLUSIONS: Sperm yields/g testis were significantly decreased in men post-vasectomy and in men with OA, relative to fertile men. Significant reductions were also observed in early (40%) and mature (29%) spermatid numbers and an increase of 31% was seen in the seminiferous tubule wall (basal membrane and collagen thickness) of vasectomized men compared with fertile men. Clinical pregnancy rates in couples who had had a vasectomy were also significantly reduced. |
| doi_str_mv | 10.1093/humrep/dei138 |
| format | Article |
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Sperm can now be obtained by testicular biopsy and subsequently used in assisted conception with ICSI. The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI. METHODS: Testicular tissue was obtained by Trucut needle from men who had undergone a vasectomy >5 years previously or had OA from other causes and from fertile men during vasectomy. Seminiferous tubules were milked to measure sperm yields. Numbers of Sertoli cells and spermatids and thickness of the seminiferous tubule walls were assessed using quantitative computerized analysis. RESULTS and CONCLUSIONS: Sperm yields/g testis were significantly decreased in men post-vasectomy and in men with OA, relative to fertile men. Significant reductions were also observed in early (40%) and mature (29%) spermatid numbers and an increase of 31% was seen in the seminiferous tubule wall (basal membrane and collagen thickness) of vasectomized men compared with fertile men. Clinical pregnancy rates in couples who had had a vasectomy were also significantly reduced.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/dei138</identifier><identifier>PMID: 15958397</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; Biological and medical sciences ; Biopsy ; clinical pregnancy rate ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; ICSI ; Male ; Medical sciences ; Middle Aged ; Oligospermia - therapy ; Pregnancy ; Pregnancy Rate ; Seminiferous Tubules - pathology ; Sertoli Cells - pathology ; Sperm Count ; Sperm Injections, Intracytoplasmic - methods ; sperm yield ; spermatids ; Spermatids - pathology ; Spermatogenesis ; Spermatozoa - pathology ; Testis - pathology ; Vasectomy ; Vasovasostomy</subject><ispartof>Human reproduction (Oxford), 2005-10, Vol.20 (10), p.2795-2800</ispartof><rights>The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Oct 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-dea733dcd8874ce6d2ba6e7a0b4978d74e264be5d84c03a9416c17f6678ddd973</citedby><cites>FETCH-LOGICAL-c458t-dea733dcd8874ce6d2ba6e7a0b4978d74e264be5d84c03a9416c17f6678ddd973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17154629$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15958397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McVicar, C.M.</creatorcontrib><creatorcontrib>O’Neill, D.A.</creatorcontrib><creatorcontrib>McClure, N.</creatorcontrib><creatorcontrib>Clements, B.</creatorcontrib><creatorcontrib>McCullough, S.</creatorcontrib><creatorcontrib>Lewis, S.E.M.</creatorcontrib><title>Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: Each year 40 000 men have a vasectomy in the UK whilst another 2400 request a reversal to begin a second family. Sperm can now be obtained by testicular biopsy and subsequently used in assisted conception with ICSI. The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI. METHODS: Testicular tissue was obtained by Trucut needle from men who had undergone a vasectomy >5 years previously or had OA from other causes and from fertile men during vasectomy. Seminiferous tubules were milked to measure sperm yields. Numbers of Sertoli cells and spermatids and thickness of the seminiferous tubule walls were assessed using quantitative computerized analysis. RESULTS and CONCLUSIONS: Sperm yields/g testis were significantly decreased in men post-vasectomy and in men with OA, relative to fertile men. Significant reductions were also observed in early (40%) and mature (29%) spermatid numbers and an increase of 31% was seen in the seminiferous tubule wall (basal membrane and collagen thickness) of vasectomized men compared with fertile men. Clinical pregnancy rates in couples who had had a vasectomy were also significantly reduced.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>clinical pregnancy rate</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>ICSI</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Oligospermia - therapy</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Seminiferous Tubules - pathology</subject><subject>Sertoli Cells - pathology</subject><subject>Sperm Count</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>sperm yield</subject><subject>spermatids</subject><subject>Spermatids - pathology</subject><subject>Spermatogenesis</subject><subject>Spermatozoa - pathology</subject><subject>Testis - pathology</subject><subject>Vasectomy</subject><subject>Vasovasostomy</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFr1zAUB_AgDvfb9OhVguDwUpc0bZIex2-b-8FPPKgoXkKavLrMtqlJOrf_3kjLBl485UE-efnyHkIvKXlHScNOr-chwHRqwVEmn6ANrTgpSlaTp2hDSi4LSjk9REcx3hCSS8mfoUNaN7VkjdigcNF1YFLEvsO3OubSD_fYjzhOEAad_A8YIbqI9WhxByG53qUM5mT8AFh3CQJOEJMzc6_D8gzDXQraJJf7ZNa6ESz-7dI13m0_7Z6jg073EV6s5zH6cnnxeXtV7D--323P9oWpapkKC1owZo2VUlQGuC1bzUFo0laNkFZUUPKqhdrKyhCmm4pyQ0XHeb60thHsGJ0sfafgf805ohpcNND3egQ_R8UlJ5KWJMPX_8AbP4cxZ1MlpVKUhNcZFQsywccYoFNTcIMO94oS9XcTatmEWjaR_au16dwOYB_1OvoM3qxAR6P7LujRuPjoBK0rXjbZvV2cn6f__rlmdDHB3QPW4afigolaXX37ri735-wr21L1gf0BPguySg</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>McVicar, C.M.</creator><creator>O’Neill, D.A.</creator><creator>McClure, N.</creator><creator>Clements, B.</creator><creator>McCullough, S.</creator><creator>Lewis, S.E.M.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI</title><author>McVicar, C.M. ; O’Neill, D.A. ; McClure, N. ; Clements, B. ; McCullough, S. ; Lewis, S.E.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-dea733dcd8874ce6d2ba6e7a0b4978d74e264be5d84c03a9416c17f6678ddd973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>clinical pregnancy rate</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>ICSI</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Oligospermia - therapy</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Seminiferous Tubules - pathology</topic><topic>Sertoli Cells - pathology</topic><topic>Sperm Count</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>sperm yield</topic><topic>spermatids</topic><topic>Spermatids - pathology</topic><topic>Spermatogenesis</topic><topic>Spermatozoa - pathology</topic><topic>Testis - pathology</topic><topic>Vasectomy</topic><topic>Vasovasostomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McVicar, C.M.</creatorcontrib><creatorcontrib>O’Neill, D.A.</creatorcontrib><creatorcontrib>McClure, N.</creatorcontrib><creatorcontrib>Clements, B.</creatorcontrib><creatorcontrib>McCullough, S.</creatorcontrib><creatorcontrib>Lewis, S.E.M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McVicar, C.M.</au><au>O’Neill, D.A.</au><au>McClure, N.</au><au>Clements, B.</au><au>McCullough, S.</au><au>Lewis, S.E.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>20</volume><issue>10</issue><spage>2795</spage><epage>2800</epage><pages>2795-2800</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: Each year 40 000 men have a vasectomy in the UK whilst another 2400 request a reversal to begin a second family. Sperm can now be obtained by testicular biopsy and subsequently used in assisted conception with ICSI. The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI. METHODS: Testicular tissue was obtained by Trucut needle from men who had undergone a vasectomy >5 years previously or had OA from other causes and from fertile men during vasectomy. Seminiferous tubules were milked to measure sperm yields. Numbers of Sertoli cells and spermatids and thickness of the seminiferous tubule walls were assessed using quantitative computerized analysis. RESULTS and CONCLUSIONS: Sperm yields/g testis were significantly decreased in men post-vasectomy and in men with OA, relative to fertile men. Significant reductions were also observed in early (40%) and mature (29%) spermatid numbers and an increase of 31% was seen in the seminiferous tubule wall (basal membrane and collagen thickness) of vasectomized men compared with fertile men. Clinical pregnancy rates in couples who had had a vasectomy were also significantly reduced.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15958397</pmid><doi>10.1093/humrep/dei138</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Biological and medical sciences Biopsy clinical pregnancy rate Female Gynecology. Andrology. Obstetrics Humans ICSI Male Medical sciences Middle Aged Oligospermia - therapy Pregnancy Pregnancy Rate Seminiferous Tubules - pathology Sertoli Cells - pathology Sperm Count Sperm Injections, Intracytoplasmic - methods sperm yield spermatids Spermatids - pathology Spermatogenesis Spermatozoa - pathology Testis - pathology Vasectomy Vasovasostomy |
| title | Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI |
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