A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer
Cancer-testis (CT) antigens are expressed in tumors but not normal tissues except the testis and could be targets for vaccine therapy in epithelial ovarian cancer (EOC). A-kinase anchoring protein 3 (AKAP-3) is a novel member of the CT antigen family. The aim of this study was to examine the express...
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| Veröffentlicht in: | Gynecologic oncology 2005-10, Vol.99 (1), p.183-188 |
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| creator | Sharma, Sameer Qian, Feng Keitz, Bernadette Driscoll, Deborah Scanlan, Mathew J. Skipper, Jonathan Rodabaugh, Kerry Lele, Shashikant Old, Lloyd J. Odunsi, Kunle |
| description | Cancer-testis (CT) antigens are expressed in tumors but not normal tissues except the testis and could be targets for vaccine therapy in epithelial ovarian cancer (EOC). A-kinase anchoring protein 3 (AKAP-3) is a novel member of the CT antigen family. The aim of this study was to examine the expression of AKAP-3 in EOC and correlate with clinico-pathologic characteristics.
One step RT-PCR was performed with RNA from normal and ovarian cancer cell lines and 74 epithelial ovarian tumor tissues. AKAP-3-specific PCR product was amplified. The distribution of AKAP-3 expression and clinico-pathologic variables was analyzed. Survival distributions were estimated, and multivariate analyses were performed.
AKAP-3 mRNA expression was demonstrated in 43/74 (58%) of the ovarian cancer specimens. AKAP-3 was expressed in normal testis, but not in other normal tissues. AKAP-3 expression significantly correlated with increased likelihood of residual tumor (
P = 0.005), but no increase in the likelihood of recurrence or persistent disease (
P = 0.06). Patients with AKAP-3 mRNA expression were found to have a significantly poorer overall survival (median 50 months) compared with patients without AKAP-3 expression (median not reached) (
P = 0.007). Multivariate analysis of AKAP-3 expression, residual disease, and response to frontline chemotherapy found response to be the strongest predictor of overall survival (
P = 0.012).
Our data demonstrate that AKAP-3 is expressed at high frequency in patients with EOC. Since AKAP-3 demonstrates tumor-restricted expression and appears to be associated with worse overall survival, it could represent an attractive target for antigen-specific immunotherapy in EOC. |
| doi_str_mv | 10.1016/j.ygyno.2005.06.006 |
| format | Article |
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One step RT-PCR was performed with RNA from normal and ovarian cancer cell lines and 74 epithelial ovarian tumor tissues. AKAP-3-specific PCR product was amplified. The distribution of AKAP-3 expression and clinico-pathologic variables was analyzed. Survival distributions were estimated, and multivariate analyses were performed.
AKAP-3 mRNA expression was demonstrated in 43/74 (58%) of the ovarian cancer specimens. AKAP-3 was expressed in normal testis, but not in other normal tissues. AKAP-3 expression significantly correlated with increased likelihood of residual tumor (
P = 0.005), but no increase in the likelihood of recurrence or persistent disease (
P = 0.06). Patients with AKAP-3 mRNA expression were found to have a significantly poorer overall survival (median 50 months) compared with patients without AKAP-3 expression (median not reached) (
P = 0.007). Multivariate analysis of AKAP-3 expression, residual disease, and response to frontline chemotherapy found response to be the strongest predictor of overall survival (
P = 0.012).
Our data demonstrate that AKAP-3 is expressed at high frequency in patients with EOC. Since AKAP-3 demonstrates tumor-restricted expression and appears to be associated with worse overall survival, it could represent an attractive target for antigen-specific immunotherapy in EOC.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1016/j.ygyno.2005.06.006</identifier><identifier>PMID: 16005946</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>A Kinase Anchor Proteins ; Adaptor Proteins, Signal Transducing - biosynthesis ; Adaptor Proteins, Signal Transducing - genetics ; Adult ; Aged ; Aged, 80 and over ; AKAP-3 ; Cancer-testis antigen ; Epithelial Cells - pathology ; Female ; Humans ; Immunotherapy ; Middle Aged ; Neoplasm Staging ; Ovarian cancer ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Prognosis ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Survival Rate</subject><ispartof>Gynecologic oncology, 2005-10, Vol.99 (1), p.183-188</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-34de4cdf08db83d8f0790a2fedac9afe5a81c5d22bb92b1b14c938bea6e175d23</citedby><cites>FETCH-LOGICAL-c357t-34de4cdf08db83d8f0790a2fedac9afe5a81c5d22bb92b1b14c938bea6e175d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygyno.2005.06.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16005946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Sameer</creatorcontrib><creatorcontrib>Qian, Feng</creatorcontrib><creatorcontrib>Keitz, Bernadette</creatorcontrib><creatorcontrib>Driscoll, Deborah</creatorcontrib><creatorcontrib>Scanlan, Mathew J.</creatorcontrib><creatorcontrib>Skipper, Jonathan</creatorcontrib><creatorcontrib>Rodabaugh, Kerry</creatorcontrib><creatorcontrib>Lele, Shashikant</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><creatorcontrib>Odunsi, Kunle</creatorcontrib><title>A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>Cancer-testis (CT) antigens are expressed in tumors but not normal tissues except the testis and could be targets for vaccine therapy in epithelial ovarian cancer (EOC). A-kinase anchoring protein 3 (AKAP-3) is a novel member of the CT antigen family. The aim of this study was to examine the expression of AKAP-3 in EOC and correlate with clinico-pathologic characteristics.
One step RT-PCR was performed with RNA from normal and ovarian cancer cell lines and 74 epithelial ovarian tumor tissues. AKAP-3-specific PCR product was amplified. The distribution of AKAP-3 expression and clinico-pathologic variables was analyzed. Survival distributions were estimated, and multivariate analyses were performed.
AKAP-3 mRNA expression was demonstrated in 43/74 (58%) of the ovarian cancer specimens. AKAP-3 was expressed in normal testis, but not in other normal tissues. AKAP-3 expression significantly correlated with increased likelihood of residual tumor (
P = 0.005), but no increase in the likelihood of recurrence or persistent disease (
P = 0.06). Patients with AKAP-3 mRNA expression were found to have a significantly poorer overall survival (median 50 months) compared with patients without AKAP-3 expression (median not reached) (
P = 0.007). Multivariate analysis of AKAP-3 expression, residual disease, and response to frontline chemotherapy found response to be the strongest predictor of overall survival (
P = 0.012).
Our data demonstrate that AKAP-3 is expressed at high frequency in patients with EOC. Since AKAP-3 demonstrates tumor-restricted expression and appears to be associated with worse overall survival, it could represent an attractive target for antigen-specific immunotherapy in EOC.</description><subject>A Kinase Anchor Proteins</subject><subject>Adaptor Proteins, Signal Transducing - biosynthesis</subject><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>AKAP-3</subject><subject>Cancer-testis antigen</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Prognosis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Survival Rate</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVoSNw0v6BQdOptt6P9kKVDDyYkbSEkENKz0Eqztty1tJHWbvzvI8eG3noSvLzPjOYh5DODkgHj39blfrn3oawA2hJ4CcDPyIyBbAsuWvmBzAAkFKJqxSX5mNIaAGpg1QW5ZDwzsuEz8rIo_jivE1LtzSpE55d0jGFC52lNN5gS-iVG-vSwoPg6xhy44KkJMeKgJ0z0r5tWdAwhHrilD8klmmEcc46D0wMNOx2dzlBegfETOe_1kPD69F6R33e3zzc_i_vHH79uFveFqdv5VNSNxcbYHoTtRG1FD3MJuurRaiN1j60WzLS2qrpOVh3rWGNkLTrUHNk85_UV-Xqcm7_1ssU0qY1LBodBewzbpLjgwKXguVgfiyaGlCL2aoxuo-NeMVAH02qt3k2rg2kFXGXTmfpyGr_tNmj_MSe1ufD9WMB85M5hVMk4zAasi2gmZYP774I3JciT2g</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Sharma, Sameer</creator><creator>Qian, Feng</creator><creator>Keitz, Bernadette</creator><creator>Driscoll, Deborah</creator><creator>Scanlan, Mathew J.</creator><creator>Skipper, Jonathan</creator><creator>Rodabaugh, Kerry</creator><creator>Lele, Shashikant</creator><creator>Old, Lloyd J.</creator><creator>Odunsi, Kunle</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer</title><author>Sharma, Sameer ; Qian, Feng ; Keitz, Bernadette ; Driscoll, Deborah ; Scanlan, Mathew J. ; Skipper, Jonathan ; Rodabaugh, Kerry ; Lele, Shashikant ; Old, Lloyd J. ; Odunsi, Kunle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-34de4cdf08db83d8f0790a2fedac9afe5a81c5d22bb92b1b14c938bea6e175d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>A Kinase Anchor Proteins</topic><topic>Adaptor Proteins, Signal Transducing - biosynthesis</topic><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>AKAP-3</topic><topic>Cancer-testis antigen</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunotherapy</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Prognosis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Sameer</creatorcontrib><creatorcontrib>Qian, Feng</creatorcontrib><creatorcontrib>Keitz, Bernadette</creatorcontrib><creatorcontrib>Driscoll, Deborah</creatorcontrib><creatorcontrib>Scanlan, Mathew J.</creatorcontrib><creatorcontrib>Skipper, Jonathan</creatorcontrib><creatorcontrib>Rodabaugh, Kerry</creatorcontrib><creatorcontrib>Lele, Shashikant</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><creatorcontrib>Odunsi, Kunle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Sameer</au><au>Qian, Feng</au><au>Keitz, Bernadette</au><au>Driscoll, Deborah</au><au>Scanlan, Mathew J.</au><au>Skipper, Jonathan</au><au>Rodabaugh, Kerry</au><au>Lele, Shashikant</au><au>Old, Lloyd J.</au><au>Odunsi, Kunle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>99</volume><issue>1</issue><spage>183</spage><epage>188</epage><pages>183-188</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><abstract>Cancer-testis (CT) antigens are expressed in tumors but not normal tissues except the testis and could be targets for vaccine therapy in epithelial ovarian cancer (EOC). A-kinase anchoring protein 3 (AKAP-3) is a novel member of the CT antigen family. The aim of this study was to examine the expression of AKAP-3 in EOC and correlate with clinico-pathologic characteristics.
One step RT-PCR was performed with RNA from normal and ovarian cancer cell lines and 74 epithelial ovarian tumor tissues. AKAP-3-specific PCR product was amplified. The distribution of AKAP-3 expression and clinico-pathologic variables was analyzed. Survival distributions were estimated, and multivariate analyses were performed.
AKAP-3 mRNA expression was demonstrated in 43/74 (58%) of the ovarian cancer specimens. AKAP-3 was expressed in normal testis, but not in other normal tissues. AKAP-3 expression significantly correlated with increased likelihood of residual tumor (
P = 0.005), but no increase in the likelihood of recurrence or persistent disease (
P = 0.06). Patients with AKAP-3 mRNA expression were found to have a significantly poorer overall survival (median 50 months) compared with patients without AKAP-3 expression (median not reached) (
P = 0.007). Multivariate analysis of AKAP-3 expression, residual disease, and response to frontline chemotherapy found response to be the strongest predictor of overall survival (
P = 0.012).
Our data demonstrate that AKAP-3 is expressed at high frequency in patients with EOC. Since AKAP-3 demonstrates tumor-restricted expression and appears to be associated with worse overall survival, it could represent an attractive target for antigen-specific immunotherapy in EOC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16005946</pmid><doi>10.1016/j.ygyno.2005.06.006</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0090-8258 |
| ispartof | Gynecologic oncology, 2005-10, Vol.99 (1), p.183-188 |
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| subjects | A Kinase Anchor Proteins Adaptor Proteins, Signal Transducing - biosynthesis Adaptor Proteins, Signal Transducing - genetics Adult Aged Aged, 80 and over AKAP-3 Cancer-testis antigen Epithelial Cells - pathology Female Humans Immunotherapy Middle Aged Neoplasm Staging Ovarian cancer Ovarian Neoplasms - genetics Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Prognosis RNA, Messenger - biosynthesis RNA, Messenger - genetics Survival Rate |
| title | A-kinase anchoring protein 3 messenger RNA expression correlates with poor prognosis in epithelial ovarian cancer |
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