Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)
There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ERα and ERβ), and in some species the Esr2 subtype has two forms, Esr2b (formerly ERβ1) and Esr2a (formerly ERβ2 or ERγ). There is little information, however, on the different characteristics and functional signific...
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| Veröffentlicht in: | Biology of reproduction 2005-10, Vol.73 (4), p.648-662 |
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| description | There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ERα and ERβ), and in some species the Esr2
subtype has two forms, Esr2b (formerly ERβ1) and Esr2a (formerly ERβ2 or ERγ). There is little information, however, on the
different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant
for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs
for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas ), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed
some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine,
and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male
fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in
fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.
Abstract
The cloning and distinct expression profiles of estrogen receptors esr1, esr2a and esr2b in a teleost species provide clues into the roles of the different Esr's in reproduction in fish |
| doi_str_mv | 10.1095/biolreprod.105.039701 |
| format | Article |
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subtype has two forms, Esr2b (formerly ERβ1) and Esr2a (formerly ERβ2 or ERγ). There is little information, however, on the
different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant
for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs
for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas ), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed
some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine,
and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male
fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in
fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.
Abstract
The cloning and distinct expression profiles of estrogen receptors esr1, esr2a and esr2b in a teleost species provide clues into the roles of the different Esr's in reproduction in fish</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.105.039701</identifier><identifier>PMID: 15930325</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Cloning, Molecular ; Cyprinidae - genetics ; Cyprinidae - growth & development ; DNA, Complementary ; Estradiol - pharmacology ; Estrogen Receptor alpha - drug effects ; Estrogen Receptor alpha - genetics ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - drug effects ; Estrogen Receptor beta - genetics ; Estrogen Receptor beta - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Hormone metabolism and regulation ; Male ; Mammalian female genital system ; Molecular Sequence Data ; Pimephales promelas ; Polymerase Chain Reaction - methods ; Sequence Homology, Amino Acid ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2005-10, Vol.73 (4), p.648-662</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17126836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15930325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FILBY, A. L</creatorcontrib><creatorcontrib>TYLER, C. R</creatorcontrib><title>Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ERα and ERβ), and in some species the Esr2
subtype has two forms, Esr2b (formerly ERβ1) and Esr2a (formerly ERβ2 or ERγ). There is little information, however, on the
different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant
for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs
for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas ), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed
some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine,
and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male
fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in
fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.
Abstract
The cloning and distinct expression profiles of estrogen receptors esr1, esr2a and esr2b in a teleost species provide clues into the roles of the different Esr's in reproduction in fish</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Cyprinidae - genetics</subject><subject>Cyprinidae - growth & development</subject><subject>DNA, Complementary</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Receptor alpha - drug effects</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - drug effects</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Hormone metabolism and regulation</subject><subject>Male</subject><subject>Mammalian female genital system</subject><subject>Molecular Sequence Data</subject><subject>Pimephales promelas</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO1DAMhiMEYoeFRwDlAgJpuyRx07RHNJoFpF3tCg3nym3dbVDalKRVgTfhbekMAxy52LL9-bdsM_ZcikspCv22st4FGoNv1lhfCiiMkA_YRmpVJEZl-UO2EUJkCUAGZ-xJjF-EkCkoeMzOpC5AgNIb9vPGO6pnh4FvOwxYTxTsD5ysH7hv-S5Owd_TwD9RTePkQ-Tygiu84Dg0XFVHt-_IBr63Mc50TNwO06HJ1nz3bQwU40HtLvjWOorcDvwKp46w4Td2GPzCX9_ZnsYOD9V1o54cxjdP2aMWXaRnJ3_OPl_t9tsPyfXt-4_bd9dJB0JMicnzVOgUalG1CqVOqzavoEVSyiihwGipTXYMshSayshqtVJnhHmbyxzO2avfuuvkrzPFqextrMk5HMjPsczyTECaFv8FpYFCiMKs4IsTOFc9NeUYbI_he_nn6ivw8gRgrNG1AYfaxn-ckev_1rf95Tp73y02UBl7dG6VhXJZFgNlWmZpDr8AwRCghw</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>FILBY, A. L</creator><creator>TYLER, C. R</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)</title><author>FILBY, A. L ; TYLER, C. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h300t-78840543c0bf2a154bf8b3fae227202375157622720643db71b3db156ea8f8183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Cyprinidae - genetics</topic><topic>Cyprinidae - growth & development</topic><topic>DNA, Complementary</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Receptor alpha - drug effects</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - drug effects</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Hormone metabolism and regulation</topic><topic>Male</topic><topic>Mammalian female genital system</topic><topic>Molecular Sequence Data</topic><topic>Pimephales promelas</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sequence Homology, Amino Acid</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FILBY, A. L</creatorcontrib><creatorcontrib>TYLER, C. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FILBY, A. L</au><au>TYLER, C. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>73</volume><issue>4</issue><spage>648</spage><epage>662</epage><pages>648-662</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ERα and ERβ), and in some species the Esr2
subtype has two forms, Esr2b (formerly ERβ1) and Esr2a (formerly ERβ2 or ERγ). There is little information, however, on the
different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant
for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs
for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas ), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed
some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine,
and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male
fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in
fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.
Abstract
The cloning and distinct expression profiles of estrogen receptors esr1, esr2a and esr2b in a teleost species provide clues into the roles of the different Esr's in reproduction in fish</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15930325</pmid><doi>10.1095/biolreprod.105.039701</doi><tpages>15</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2005-10, Vol.73 (4), p.648-662 |
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| language | eng |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Cloning, Molecular Cyprinidae - genetics Cyprinidae - growth & development DNA, Complementary Estradiol - pharmacology Estrogen Receptor alpha - drug effects Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen Receptor beta - drug effects Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Hormone metabolism and regulation Male Mammalian female genital system Molecular Sequence Data Pimephales promelas Polymerase Chain Reaction - methods Sequence Homology, Amino Acid Vertebrates: reproduction |
| title | Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas) |
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