Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy
Current diagnosis of peanut allergy relies on natural extracts that lack standardization. Recombinant DNA technology allows production of pure biochemically characterized proteins. Their usefulness for peanut allergy diagnosis is not established. This study aimed to evaluate the diagnostic value of...
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| Veröffentlicht in: | Journal of Allergy and Clinical Immunology 2006-07, Vol.118 (1), p.250-256 |
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| creator | Astier, Catherine Morisset, Martine Roitel, Olivier Codreanu, Fanny Jacquenet, Sandrine Franck, Patricia Ogier, Virginie Petit, Nicolas Proust, Barbara Moneret-Vautrin, Denise-Anne Burks, A. Wesley Bihain, Bernard Sampson, Hugh A. Kanny, Gisèle |
| description | Current diagnosis of peanut allergy relies on natural extracts that lack standardization. Recombinant DNA technology allows production of pure biochemically characterized proteins. Their usefulness for peanut allergy diagnosis is not established.
This study aimed to evaluate the diagnostic value of the 3 major recombinant peanut allergens.
Recombinant (r) Ara h 1, rAra h 2, and rAra h 3 were produced according to the recommendations of good manufacturing practice for recombinant allergens. Skin prick tests (SPTs) and IgE ELISA assays were performed in 30 patients with peanut allergy and 30 control subjects without food allergy: 15 nonatopic and 15 sensitized to birch pollen. Disease severity was graded by clinical scoring.
All patients with peanut allergy showed positive SPT results to rAra h 2; 40% reacted with rAra h 1 and 27% with rAra h 3. No control subjects reacted with any of the recombinant allergens. Monosensitization to rAra h 2 was observed in 53% of patients. Neither SPT size nor levels of specific IgE were correlated with the disease severity. However, patients with monosensitization to rAra h 2 had a significantly lower severity score than polysensitized subjects and a lower level of specific IgE against peanut extract and rAra h 2.
Skin prick tests to individual recombinant peanut allergens appear to be a safe and effective diagnostic tool. Cosensitization to rAra h 2 and rArah 1 and/or rAra h 3 is predictive of more severe reactions.
Recombinant peanut allergens can be used by SPTs for diagnosis and evaluation of allergy severity. |
| doi_str_mv | 10.1016/j.jaci.2006.04.053 |
| format | Article |
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This study aimed to evaluate the diagnostic value of the 3 major recombinant peanut allergens.
Recombinant (r) Ara h 1, rAra h 2, and rAra h 3 were produced according to the recommendations of good manufacturing practice for recombinant allergens. Skin prick tests (SPTs) and IgE ELISA assays were performed in 30 patients with peanut allergy and 30 control subjects without food allergy: 15 nonatopic and 15 sensitized to birch pollen. Disease severity was graded by clinical scoring.
All patients with peanut allergy showed positive SPT results to rAra h 2; 40% reacted with rAra h 1 and 27% with rAra h 3. No control subjects reacted with any of the recombinant allergens. Monosensitization to rAra h 2 was observed in 53% of patients. Neither SPT size nor levels of specific IgE were correlated with the disease severity. However, patients with monosensitization to rAra h 2 had a significantly lower severity score than polysensitized subjects and a lower level of specific IgE against peanut extract and rAra h 2.
Skin prick tests to individual recombinant peanut allergens appear to be a safe and effective diagnostic tool. Cosensitization to rAra h 2 and rArah 1 and/or rAra h 3 is predictive of more severe reactions.
Recombinant peanut allergens can be used by SPTs for diagnosis and evaluation of allergy severity.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.jaci.2006.04.053</identifier><identifier>PMID: 16815163</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adolescent ; Adult ; Allergens - immunology ; Allergic diseases ; Allergies ; Arachis - immunology ; Arachis hypogaea ; Asthma ; Biological and medical sciences ; Child ; Child, Preschool ; Cross Reactions ; diagnosis ; Digestive allergic diseases ; E coli ; Female ; Food ; Food allergies ; Food allergy ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunoglobulin E - blood ; Immunopathology ; Male ; Medical sciences ; peanut ; Peanut Hypersensitivity - diagnosis ; Peanuts ; Predictive Value of Tests ; recombinant allergens ; Recombinant Proteins - immunology ; Skin Tests</subject><ispartof>Journal of Allergy and Clinical Immunology, 2006-07, Vol.118 (1), p.250-256</ispartof><rights>2006 American Academy of Allergy, Asthma and Immunology</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Elsevier Limited Jul 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-5db1532b565b3fa817c023668eaf5f082355345599cbb4ecda719b9c0221e51f3</citedby><cites>FETCH-LOGICAL-c509t-5db1532b565b3fa817c023668eaf5f082355345599cbb4ecda719b9c0221e51f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0091674906010530$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17964995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16815163$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Astier, Catherine</creatorcontrib><creatorcontrib>Morisset, Martine</creatorcontrib><creatorcontrib>Roitel, Olivier</creatorcontrib><creatorcontrib>Codreanu, Fanny</creatorcontrib><creatorcontrib>Jacquenet, Sandrine</creatorcontrib><creatorcontrib>Franck, Patricia</creatorcontrib><creatorcontrib>Ogier, Virginie</creatorcontrib><creatorcontrib>Petit, Nicolas</creatorcontrib><creatorcontrib>Proust, Barbara</creatorcontrib><creatorcontrib>Moneret-Vautrin, Denise-Anne</creatorcontrib><creatorcontrib>Burks, A. Wesley</creatorcontrib><creatorcontrib>Bihain, Bernard</creatorcontrib><creatorcontrib>Sampson, Hugh A.</creatorcontrib><creatorcontrib>Kanny, Gisèle</creatorcontrib><title>Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy</title><title>Journal of Allergy and Clinical Immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>Current diagnosis of peanut allergy relies on natural extracts that lack standardization. Recombinant DNA technology allows production of pure biochemically characterized proteins. Their usefulness for peanut allergy diagnosis is not established.
This study aimed to evaluate the diagnostic value of the 3 major recombinant peanut allergens.
Recombinant (r) Ara h 1, rAra h 2, and rAra h 3 were produced according to the recommendations of good manufacturing practice for recombinant allergens. Skin prick tests (SPTs) and IgE ELISA assays were performed in 30 patients with peanut allergy and 30 control subjects without food allergy: 15 nonatopic and 15 sensitized to birch pollen. Disease severity was graded by clinical scoring.
All patients with peanut allergy showed positive SPT results to rAra h 2; 40% reacted with rAra h 1 and 27% with rAra h 3. No control subjects reacted with any of the recombinant allergens. Monosensitization to rAra h 2 was observed in 53% of patients. Neither SPT size nor levels of specific IgE were correlated with the disease severity. However, patients with monosensitization to rAra h 2 had a significantly lower severity score than polysensitized subjects and a lower level of specific IgE against peanut extract and rAra h 2.
Skin prick tests to individual recombinant peanut allergens appear to be a safe and effective diagnostic tool. Cosensitization to rAra h 2 and rArah 1 and/or rAra h 3 is predictive of more severe reactions.
Recombinant peanut allergens can be used by SPTs for diagnosis and evaluation of allergy severity.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Allergies</subject><subject>Arachis - immunology</subject><subject>Arachis hypogaea</subject><subject>Asthma</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross Reactions</subject><subject>diagnosis</subject><subject>Digestive allergic diseases</subject><subject>E coli</subject><subject>Female</subject><subject>Food</subject><subject>Food allergies</subject><subject>Food allergy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunoglobulin E - blood</subject><subject>Immunopathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>peanut</subject><subject>Peanut Hypersensitivity - diagnosis</subject><subject>Peanuts</subject><subject>Predictive Value of Tests</subject><subject>recombinant allergens</subject><subject>Recombinant Proteins - immunology</subject><subject>Skin Tests</subject><issn>0091-6749</issn><issn>1097-6825</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFq3DAQhkVpaTZpX6CHIijtzY7GsmQLegmhTQuB5tCeVVkeL3K80layF_L2ldktgR6S0zDw_cPMfIS8A1YCA3k5lqOxrqwYkyWrSyb4C7IBpppCtpV4STaMKShkU6szcp7SyHLPW_WanIFsQYDkG_L7LmLv7OwOSA9mWpCGgaZ75-k-OntPZ0xzoktyfksj2rDrnDd-pmaaMG7RJzqESHtntj4kl9b0Ho1f_hEPb8irwUwJ357qBfn19cvP62_F7Y-b79dXt4UVTM2F6DsQvOqEFB0fTAuNZRWXskUziIG1FReC10IoZbuuRtubBlSnMlQBChj4Bfl0nLuP4c-St9Y7lyxOk_EYlqRlKxlUvHkWBCVZW1c8gx_-A8ewRJ-P0CBY3QioYaWqI2VjSCnioPPjdiY-aGB61aRHvWrSqybNap015dD70-il22H_GDl5ycDHE2CSNdMQjbcuPXKNkrVSInOfjxzm1x4cRp2sQ2-z02xr1n1wT-3xFxjJr_s</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Astier, Catherine</creator><creator>Morisset, Martine</creator><creator>Roitel, Olivier</creator><creator>Codreanu, Fanny</creator><creator>Jacquenet, Sandrine</creator><creator>Franck, Patricia</creator><creator>Ogier, Virginie</creator><creator>Petit, Nicolas</creator><creator>Proust, Barbara</creator><creator>Moneret-Vautrin, Denise-Anne</creator><creator>Burks, A. Wesley</creator><creator>Bihain, Bernard</creator><creator>Sampson, Hugh A.</creator><creator>Kanny, Gisèle</creator><general>Mosby, Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy</title><author>Astier, Catherine ; Morisset, Martine ; Roitel, Olivier ; Codreanu, Fanny ; Jacquenet, Sandrine ; Franck, Patricia ; Ogier, Virginie ; Petit, Nicolas ; Proust, Barbara ; Moneret-Vautrin, Denise-Anne ; Burks, A. Wesley ; Bihain, Bernard ; Sampson, Hugh A. ; Kanny, Gisèle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-5db1532b565b3fa817c023668eaf5f082355345599cbb4ecda719b9c0221e51f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Allergies</topic><topic>Arachis - immunology</topic><topic>Arachis hypogaea</topic><topic>Asthma</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross Reactions</topic><topic>diagnosis</topic><topic>Digestive allergic diseases</topic><topic>E coli</topic><topic>Female</topic><topic>Food</topic><topic>Food allergies</topic><topic>Food allergy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunoglobulin E - blood</topic><topic>Immunopathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>peanut</topic><topic>Peanut Hypersensitivity - diagnosis</topic><topic>Peanuts</topic><topic>Predictive Value of Tests</topic><topic>recombinant allergens</topic><topic>Recombinant Proteins - immunology</topic><topic>Skin Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Astier, Catherine</creatorcontrib><creatorcontrib>Morisset, Martine</creatorcontrib><creatorcontrib>Roitel, Olivier</creatorcontrib><creatorcontrib>Codreanu, Fanny</creatorcontrib><creatorcontrib>Jacquenet, Sandrine</creatorcontrib><creatorcontrib>Franck, Patricia</creatorcontrib><creatorcontrib>Ogier, Virginie</creatorcontrib><creatorcontrib>Petit, Nicolas</creatorcontrib><creatorcontrib>Proust, Barbara</creatorcontrib><creatorcontrib>Moneret-Vautrin, Denise-Anne</creatorcontrib><creatorcontrib>Burks, A. 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Wesley</au><au>Bihain, Bernard</au><au>Sampson, Hugh A.</au><au>Kanny, Gisèle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy</atitle><jtitle>Journal of Allergy and Clinical Immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>118</volume><issue>1</issue><spage>250</spage><epage>256</epage><pages>250-256</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><eissn>1365-2567</eissn><coden>JACIBY</coden><abstract>Current diagnosis of peanut allergy relies on natural extracts that lack standardization. Recombinant DNA technology allows production of pure biochemically characterized proteins. Their usefulness for peanut allergy diagnosis is not established.
This study aimed to evaluate the diagnostic value of the 3 major recombinant peanut allergens.
Recombinant (r) Ara h 1, rAra h 2, and rAra h 3 were produced according to the recommendations of good manufacturing practice for recombinant allergens. Skin prick tests (SPTs) and IgE ELISA assays were performed in 30 patients with peanut allergy and 30 control subjects without food allergy: 15 nonatopic and 15 sensitized to birch pollen. Disease severity was graded by clinical scoring.
All patients with peanut allergy showed positive SPT results to rAra h 2; 40% reacted with rAra h 1 and 27% with rAra h 3. No control subjects reacted with any of the recombinant allergens. Monosensitization to rAra h 2 was observed in 53% of patients. Neither SPT size nor levels of specific IgE were correlated with the disease severity. However, patients with monosensitization to rAra h 2 had a significantly lower severity score than polysensitized subjects and a lower level of specific IgE against peanut extract and rAra h 2.
Skin prick tests to individual recombinant peanut allergens appear to be a safe and effective diagnostic tool. Cosensitization to rAra h 2 and rArah 1 and/or rAra h 3 is predictive of more severe reactions.
Recombinant peanut allergens can be used by SPTs for diagnosis and evaluation of allergy severity.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>16815163</pmid><doi>10.1016/j.jaci.2006.04.053</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Allergens - immunology Allergic diseases Allergies Arachis - immunology Arachis hypogaea Asthma Biological and medical sciences Child Child, Preschool Cross Reactions diagnosis Digestive allergic diseases E coli Female Food Food allergies Food allergy Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoglobulin E - blood Immunopathology Male Medical sciences peanut Peanut Hypersensitivity - diagnosis Peanuts Predictive Value of Tests recombinant allergens Recombinant Proteins - immunology Skin Tests |
| title | Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy |
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