Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia
To investigate the prevalence of PTCH (patched gene) and Smo (smoothened gene) expression in human pancreatic cancerous tissues and its association with clinical characteristics. A rabbit polyclonal antibody against PTCH was prepared through the immunization of prokaryotic recombinant PTCH1170-1433...
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| Veröffentlicht in: | Pancreas 2006-07, Vol.33 (1), p.38-44 |
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| creator | Shao, Jianguo Zhang, Ling Gao, Jun Li, Zhaoshen Chen, Zhirong |
| description | To investigate the prevalence of PTCH (patched gene) and Smo (smoothened gene) expression in human pancreatic cancerous tissues and its association with clinical characteristics.
A rabbit polyclonal antibody against PTCH was prepared through the immunization of prokaryotic recombinant PTCH1170-1433 protein. The PTCH and Smo expression in 39 resected pancreas specimens from 28 patients with pancreatic cancer, 6 with chronic pancreatitis (as control), and 5 with pancreatic pseudocyst (as control) were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry. The relationships between their expressions and pathological characteristics such as tumor sizes, degree of differentiation, nodal status, distant metastasis, and the blood sugar level were analyzed.
The prevalence of PTCH and Smo expressions in cancerous tissues were 71.4% (20/28) and 53.6% (15/28), respectively, whereas no expression in the nontumor pancreas tissues was found. Both PTCH and Smo expressions correlated with the low levels of tumor tissue differentiation (P < 0.05) and PTCH and Smo expressions in islet cells of cancerous tissues associated with hyperglycemia.
Because aberrant expressions of PTCH and Smo were common in human pancreatic carcinoma tissues and were associated with the low-level differentiation of tumor tissue and hyperglycemia, this indicated that these molecules played a fundamental role in pancreas tumorigenesis and were regarded as new targets for diagnosis and treatment of human pancreatic cancer. |
| doi_str_mv | 10.1097/01.mpa.0000222319.59360.21 |
| format | Article |
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A rabbit polyclonal antibody against PTCH was prepared through the immunization of prokaryotic recombinant PTCH1170-1433 protein. The PTCH and Smo expression in 39 resected pancreas specimens from 28 patients with pancreatic cancer, 6 with chronic pancreatitis (as control), and 5 with pancreatic pseudocyst (as control) were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry. The relationships between their expressions and pathological characteristics such as tumor sizes, degree of differentiation, nodal status, distant metastasis, and the blood sugar level were analyzed.
The prevalence of PTCH and Smo expressions in cancerous tissues were 71.4% (20/28) and 53.6% (15/28), respectively, whereas no expression in the nontumor pancreas tissues was found. Both PTCH and Smo expressions correlated with the low levels of tumor tissue differentiation (P < 0.05) and PTCH and Smo expressions in islet cells of cancerous tissues associated with hyperglycemia.
Because aberrant expressions of PTCH and Smo were common in human pancreatic carcinoma tissues and were associated with the low-level differentiation of tumor tissue and hyperglycemia, this indicated that these molecules played a fundamental role in pancreas tumorigenesis and were regarded as new targets for diagnosis and treatment of human pancreatic cancer.</description><identifier>ISSN: 0885-3177</identifier><identifier>EISSN: 1536-4828</identifier><identifier>DOI: 10.1097/01.mpa.0000222319.59360.21</identifier><identifier>PMID: 16804411</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Carcinoma, Islet Cell - genetics ; Carcinoma, Islet Cell - metabolism ; Carcinoma, Islet Cell - pathology ; Carcinoma, Pancreatic Ductal - genetics ; Carcinoma, Pancreatic Ductal - metabolism ; Carcinoma, Pancreatic Ductal - pathology ; Cell Differentiation ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Hyperglycemia - genetics ; Hyperglycemia - metabolism ; Hyperglycemia - pathology ; Immunohistochemistry ; Male ; Middle Aged ; Pancreatic Neoplasms - genetics ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Patched Receptors ; Patched-1 Receptor ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Smoothened Receptor</subject><ispartof>Pancreas, 2006-07, Vol.33 (1), p.38-44</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-cf918f183fbe0ca5cb134c95f79463f396558dd68f5c1babf51e9f1b896f12813</citedby><cites>FETCH-LOGICAL-c317t-cf918f183fbe0ca5cb134c95f79463f396558dd68f5c1babf51e9f1b896f12813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16804411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shao, Jianguo</creatorcontrib><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Gao, Jun</creatorcontrib><creatorcontrib>Li, Zhaoshen</creatorcontrib><creatorcontrib>Chen, Zhirong</creatorcontrib><title>Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia</title><title>Pancreas</title><addtitle>Pancreas</addtitle><description>To investigate the prevalence of PTCH (patched gene) and Smo (smoothened gene) expression in human pancreatic cancerous tissues and its association with clinical characteristics.
A rabbit polyclonal antibody against PTCH was prepared through the immunization of prokaryotic recombinant PTCH1170-1433 protein. The PTCH and Smo expression in 39 resected pancreas specimens from 28 patients with pancreatic cancer, 6 with chronic pancreatitis (as control), and 5 with pancreatic pseudocyst (as control) were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry. The relationships between their expressions and pathological characteristics such as tumor sizes, degree of differentiation, nodal status, distant metastasis, and the blood sugar level were analyzed.
The prevalence of PTCH and Smo expressions in cancerous tissues were 71.4% (20/28) and 53.6% (15/28), respectively, whereas no expression in the nontumor pancreas tissues was found. Both PTCH and Smo expressions correlated with the low levels of tumor tissue differentiation (P < 0.05) and PTCH and Smo expressions in islet cells of cancerous tissues associated with hyperglycemia.
Because aberrant expressions of PTCH and Smo were common in human pancreatic carcinoma tissues and were associated with the low-level differentiation of tumor tissue and hyperglycemia, this indicated that these molecules played a fundamental role in pancreas tumorigenesis and were regarded as new targets for diagnosis and treatment of human pancreatic cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Carcinoma, Islet Cell - genetics</subject><subject>Carcinoma, Islet Cell - metabolism</subject><subject>Carcinoma, Islet Cell - pathology</subject><subject>Carcinoma, Pancreatic Ductal - genetics</subject><subject>Carcinoma, Pancreatic Ductal - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>Cell Differentiation</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Hyperglycemia - genetics</subject><subject>Hyperglycemia - metabolism</subject><subject>Hyperglycemia - pathology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Patched Receptors</subject><subject>Patched-1 Receptor</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Signal Transduction</subject><subject>Smoothened Receptor</subject><issn>0885-3177</issn><issn>1536-4828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd9uFSEQxonR2GP1FQzxwtSLXZllYcG7pvFf0kQT9Zqw7NDFnF1W4ETPY_jG0vaYzs1M4Bu-j_wIeQWsBaaHtwzaZbMtq9V1HQfdCs0lazt4RHYguGx61anHZMeUEg2HYTgjz3L-yRgMXOin5AykYn0PsCN_L0dMya6F4p8tYc4hrjR6-vX71Sd6sdniZpzoDa74htp1ot-WSC_yEmOZ69n_m7DS-bDYlW52dQltCY66OmKKh0xLyPmA-W4_lNpzji5UUbX6HcpM5-OG6WZ_dLgE-5w88Xaf8cWpn5MfH97XNM31l4-fry6vG1d_VBrnNSgPivsRmbPCjcB7p4UfdC-551oKoaZJKi8cjHb0AlB7GJWWHjoF_Jy8vn93S_FXjVfMErLD_d6uWFMbqYTupGZV-O5e6FLMOaE3WwqLTUcDzNwCMQxMBWIegJg7IKa7dXl5cjmMC04PqycC_B9vdopX</recordid><startdate>200607</startdate><enddate>200607</enddate><creator>Shao, Jianguo</creator><creator>Zhang, Ling</creator><creator>Gao, Jun</creator><creator>Li, Zhaoshen</creator><creator>Chen, Zhirong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200607</creationdate><title>Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia</title><author>Shao, Jianguo ; Zhang, Ling ; Gao, Jun ; Li, Zhaoshen ; Chen, Zhirong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-cf918f183fbe0ca5cb134c95f79463f396558dd68f5c1babf51e9f1b896f12813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Carcinoma, Islet Cell - genetics</topic><topic>Carcinoma, Islet Cell - metabolism</topic><topic>Carcinoma, Islet Cell - pathology</topic><topic>Carcinoma, Pancreatic Ductal - genetics</topic><topic>Carcinoma, Pancreatic Ductal - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - pathology</topic><topic>Cell Differentiation</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Hyperglycemia - genetics</topic><topic>Hyperglycemia - metabolism</topic><topic>Hyperglycemia - pathology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Patched Receptors</topic><topic>Patched-1 Receptor</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Signal Transduction</topic><topic>Smoothened Receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shao, Jianguo</creatorcontrib><creatorcontrib>Zhang, Ling</creatorcontrib><creatorcontrib>Gao, Jun</creatorcontrib><creatorcontrib>Li, Zhaoshen</creatorcontrib><creatorcontrib>Chen, Zhirong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pancreas</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shao, Jianguo</au><au>Zhang, Ling</au><au>Gao, Jun</au><au>Li, Zhaoshen</au><au>Chen, Zhirong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia</atitle><jtitle>Pancreas</jtitle><addtitle>Pancreas</addtitle><date>2006-07</date><risdate>2006</risdate><volume>33</volume><issue>1</issue><spage>38</spage><epage>44</epage><pages>38-44</pages><issn>0885-3177</issn><eissn>1536-4828</eissn><abstract>To investigate the prevalence of PTCH (patched gene) and Smo (smoothened gene) expression in human pancreatic cancerous tissues and its association with clinical characteristics.
A rabbit polyclonal antibody against PTCH was prepared through the immunization of prokaryotic recombinant PTCH1170-1433 protein. The PTCH and Smo expression in 39 resected pancreas specimens from 28 patients with pancreatic cancer, 6 with chronic pancreatitis (as control), and 5 with pancreatic pseudocyst (as control) were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry. The relationships between their expressions and pathological characteristics such as tumor sizes, degree of differentiation, nodal status, distant metastasis, and the blood sugar level were analyzed.
The prevalence of PTCH and Smo expressions in cancerous tissues were 71.4% (20/28) and 53.6% (15/28), respectively, whereas no expression in the nontumor pancreas tissues was found. Both PTCH and Smo expressions correlated with the low levels of tumor tissue differentiation (P < 0.05) and PTCH and Smo expressions in islet cells of cancerous tissues associated with hyperglycemia.
Because aberrant expressions of PTCH and Smo were common in human pancreatic carcinoma tissues and were associated with the low-level differentiation of tumor tissue and hyperglycemia, this indicated that these molecules played a fundamental role in pancreas tumorigenesis and were regarded as new targets for diagnosis and treatment of human pancreatic cancer.</abstract><cop>United States</cop><pmid>16804411</pmid><doi>10.1097/01.mpa.0000222319.59360.21</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Carcinoma, Islet Cell - genetics Carcinoma, Islet Cell - metabolism Carcinoma, Islet Cell - pathology Carcinoma, Pancreatic Ductal - genetics Carcinoma, Pancreatic Ductal - metabolism Carcinoma, Pancreatic Ductal - pathology Cell Differentiation Female Gene Expression Regulation, Neoplastic Humans Hyperglycemia - genetics Hyperglycemia - metabolism Hyperglycemia - pathology Immunohistochemistry Male Middle Aged Pancreatic Neoplasms - genetics Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Patched Receptors Patched-1 Receptor Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Smoothened Receptor |
| title | Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia |
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