High-efficiency gene knockdown using chimeric ribozymes in fish embryos

We report an effective gene knockdown technique in rainbow trout embryos using additional RNA components combined with ribozymes (R zs). Chimeric R zs (tR zCs) containing tRNA Val, R z against GFP, and a constitutive transport element were microinjected into transgenic embryos. tR zCs induced greate...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-10, Vol.336 (2), p.438-443
Hauptverfasser:	Boonanuntanasarn, Surintorn, Takeuchi, Toshio, Yoshizaki, Goro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified - embryology Animals, Genetically Modified - genetics Chimera - embryology Chimera - genetics Constitutive transport element Fishes - embryology Fishes - genetics Gene knockdown Gene Silencing Genetic Engineering - methods Oncorhynchus mykiss Rainbow trout Ribozyme RNA, Catalytic - genetics Transgenic fish tRNA
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