A novel neisserial shuttle plasmid: A useful new tool for meningococcal research
We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Ps...
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| Veröffentlicht in: | FEMS microbiology letters 2005-10, Vol.251 (1), p.143-147 |
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| creator | O’ Dwyer, Clíona A. Langford, Paul R. Kroll, J. Simon |
| description | We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium
Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from
Acinetobacter baumannii and pNI10 from
Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in
Escherichia coli and
Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research. |
| doi_str_mv | 10.1016/j.femsle.2005.07.036 |
| format | Article |
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Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from
Acinetobacter baumannii and pNI10 from
Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in
Escherichia coli and
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Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from
Acinetobacter baumannii and pNI10 from
Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in
Escherichia coli and
Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - genetics</subject><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>Genetics, Microbial - methods</subject><subject>Kanamycin</subject><subject>Kanamycin Resistance - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Neisseria - genetics</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - genetics</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Open Reading Frames</subject><subject>Plasmid</subject><subject>Plasmids - isolation & purification</subject><subject>Proteins</subject><subject>Pseudomonas</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Shuttle vector</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkV1rFDEUhoModq3-A5EB0bvZJpNvLwpLsSqs6EXvQyZzxmbJTNZkpqX_3gyzUPBCexUIz_vmnDwIvSV4SzARF4dtD0MOsG0w5lsst5iKZ2hDuGS10EI9RxtMpaoJ1vIMvcr5gDFmDRYv0RkRhDSK6A36uavGeAehGsHnDMnbUOXbeZoCVMdg8-C7T9WumjP08wLdV1OMoepjqgYY_fgruuhcCSXIYJO7fY1e9DZkeHM6z9HN9eebq6_1_seXb1e7fe24YrQGwrVUumW0tS0WrCWYUVtuKTQNl0Cso7oXwgmnCQWqoLVUCdY1XddxTs_Rx7X2mOLvGfJkBp8dhGBHiHM2QnGNpdb_BYmkWmm2NL7_CzzEOY1lB9NQLDjnmNJCsZVyKeacoDfH5AebHgzBZvFiDmb1YhYvBktTvJTYu1P53A7QPYZOIgrw4QTYXL6zT3Z0Pj9ycunWsnBq5e59gIcnPW6uv-8JW0a_WKNxPj516ss1AUXknYdksvMwOuh8AjeZLvp_F_wBSx_M5g</recordid><startdate>200510</startdate><enddate>200510</enddate><creator>O’ Dwyer, Clíona A.</creator><creator>Langford, Paul R.</creator><creator>Kroll, J. 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Simon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5843-e159789b43bab064b1043ae153e2257e1ac39f66c6c913e38eba3864d2ddd553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acinetobacter baumannii</topic><topic>Acinetobacter baumannii - genetics</topic><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>Genetics, Microbial - methods</topic><topic>Kanamycin</topic><topic>Kanamycin Resistance - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Neisseria - genetics</topic><topic>Neisseria meningitidis</topic><topic>Neisseria meningitidis - genetics</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Open Reading Frames</topic><topic>Plasmid</topic><topic>Plasmids - isolation & purification</topic><topic>Proteins</topic><topic>Pseudomonas</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Shuttle vector</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O’ Dwyer, Clíona A.</creatorcontrib><creatorcontrib>Langford, Paul R.</creatorcontrib><creatorcontrib>Kroll, J. 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Simon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel neisserial shuttle plasmid: A useful new tool for meningococcal research</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2005-10</date><risdate>2005</risdate><volume>251</volume><issue>1</issue><spage>143</spage><epage>147</epage><pages>143-147</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium
Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from
Acinetobacter baumannii and pNI10 from
Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in
Escherichia coli and
Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>16112819</pmid><doi>10.1016/j.femsle.2005.07.036</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2005-10, Vol.251 (1), p.143-147 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68590799 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Acinetobacter baumannii Acinetobacter baumannii - genetics Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacteriology Biological and medical sciences DNA, Bacterial - chemistry DNA, Bacterial - genetics E coli Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic Markers Genetic Vectors Genetics Genetics, Microbial - methods Kanamycin Kanamycin Resistance - genetics Microbiology Molecular Sequence Data Neisseria - genetics Neisseria meningitidis Neisseria meningitidis - genetics Nucleotide sequence Nucleotides Open Reading Frames Plasmid Plasmids - isolation & purification Proteins Pseudomonas Sequence Analysis, DNA Sequence Homology, Amino Acid Shuttle vector |
| title | A novel neisserial shuttle plasmid: A useful new tool for meningococcal research |
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