ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST
A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells de...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2005-05, Vol.41 (5), p.130-141 |
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| creator | TALBOT, NEIL C CAPERNA, THOMAS J POWELL, ANNE M EALY, ALAN D BLOMBERG, LE ANN GARRETT, WESLEY M |
| description | A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with desmosomes. Rough endoplasmic reticulum was prominent within the cells as were lipid vacuoles. Immunocytochemistry indicated the BPE-1 cells had robust microtubule networks. These cells have been grown for over 2 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The BPE-1 cell line presumably arose from embryonic cells that became diploid soon after parthenogenetic activation and development of the early embryo. However, metaphase spreads prepared at passage 41 indicated that the cell population had a hypodiploid (2n = 60) unimodal chromosome content with a mode of 53 and a median and mean of 52. The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos. |
| doi_str_mv | 10.1290/040901.1 |
| format | Article |
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The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with desmosomes. Rough endoplasmic reticulum was prominent within the cells as were lipid vacuoles. Immunocytochemistry indicated the BPE-1 cells had robust microtubule networks. These cells have been grown for over 2 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The BPE-1 cell line presumably arose from embryonic cells that became diploid soon after parthenogenetic activation and development of the early embryo. However, metaphase spreads prepared at passage 41 indicated that the cell population had a hypodiploid (2n = 60) unimodal chromosome content with a mode of 53 and a median and mean of 52. The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos.</description><identifier>ISSN: 1071-2690</identifier><identifier>ISSN: 1543-706X</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1290/040901.1</identifier><identifier>PMID: 16153145</identifier><identifier>CODEN: IVCAED</identifier><language>eng</language><publisher>Germany: Society for In Vitro Biology</publisher><subject>Animals ; Blastocyst ; Blastocyst - cytology ; bovine ; Cattle ; cell ; CELL AND TISSUE MODELS ; cell culture ; Cell culture techniques ; Cell Line - cytology ; Cell Line - ultrastructure ; Cell lines ; chromosome number ; Chromosomes, Mammalian - genetics ; Cultured cells ; Cytogenetic Analysis ; Diploidy ; Embryos ; Endoderm ; Endoderm - cytology ; Feeder cells ; Immunohistochemistry ; Interferon Type I ; line ; Microscopy, Electron, Transmission ; Parthenogenesis ; parthenogenic ; Pregnancy Proteins ; rough endoplasmic reticulum ; T lymphocytes ; tight junctions ; Ungulates ; vacuoles ; visceral endoderm cell lines</subject><ispartof>In vitro cellular & developmental biology. Animal, 2005-05, Vol.41 (5), p.130-141</ispartof><rights>Society for In Vitro Biology</rights><rights>Copyright 2005 Society for In Vitro Biology</rights><rights>Copyright Society for In Vitro Biology May/Jun 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b415t-e2120c522ae767f373599d8fdd0270e56eafdbcf3400484690d5267695f9a5433</citedby><cites>FETCH-LOGICAL-b415t-e2120c522ae767f373599d8fdd0270e56eafdbcf3400484690d5267695f9a5433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1290/040901.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4295607$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,26959,27905,27906,52344,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16153145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TALBOT, NEIL C</creatorcontrib><creatorcontrib>CAPERNA, THOMAS J</creatorcontrib><creatorcontrib>POWELL, ANNE M</creatorcontrib><creatorcontrib>EALY, ALAN D</creatorcontrib><creatorcontrib>BLOMBERG, LE ANN</creatorcontrib><creatorcontrib>GARRETT, WESLEY M</creatorcontrib><title>ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with desmosomes. Rough endoplasmic reticulum was prominent within the cells as were lipid vacuoles. Immunocytochemistry indicated the BPE-1 cells had robust microtubule networks. These cells have been grown for over 2 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The BPE-1 cell line presumably arose from embryonic cells that became diploid soon after parthenogenetic activation and development of the early embryo. However, metaphase spreads prepared at passage 41 indicated that the cell population had a hypodiploid (2n = 60) unimodal chromosome content with a mode of 53 and a median and mean of 52. The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos.</description><subject>Animals</subject><subject>Blastocyst</subject><subject>Blastocyst - cytology</subject><subject>bovine</subject><subject>Cattle</subject><subject>cell</subject><subject>CELL AND TISSUE MODELS</subject><subject>cell culture</subject><subject>Cell culture techniques</subject><subject>Cell Line - cytology</subject><subject>Cell Line - ultrastructure</subject><subject>Cell lines</subject><subject>chromosome number</subject><subject>Chromosomes, Mammalian - genetics</subject><subject>Cultured cells</subject><subject>Cytogenetic Analysis</subject><subject>Diploidy</subject><subject>Embryos</subject><subject>Endoderm</subject><subject>Endoderm - cytology</subject><subject>Feeder cells</subject><subject>Immunohistochemistry</subject><subject>Interferon Type I</subject><subject>line</subject><subject>Microscopy, Electron, Transmission</subject><subject>Parthenogenesis</subject><subject>parthenogenic</subject><subject>Pregnancy Proteins</subject><subject>rough endoplasmic reticulum</subject><subject>T lymphocytes</subject><subject>tight junctions</subject><subject>Ungulates</subject><subject>vacuoles</subject><subject>visceral endoderm cell lines</subject><issn>1071-2690</issn><issn>1543-706X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkV9v0zAUxSMEYmMg8QEQWDygvWRc27EdP3qpu0bKEpRkFfBipY2DWrVNF7cPfPu5SgUICeEX_zm_e-3jEwRvMdxgIuEzRCAB3-BnwSVmEQ0F8K_P_RoEDgmXcBG8cm4NfkjMXwYXmGNGccQug31aFZmq0yJHKp-gZKZKldS6TL-Ph8UUKXRbzNNco3laJbpUGdL5pJjo8h4lOstQdtL8Np3rCZqWxb2v-KLKeqbz4k7nuk4TdJupqi6Sb1X9OnjRNRtn35znq-BhqutkFmbFXZqoLFxEmB1CSzCBJSOksYKLjgrKpGzjrm2BCLCM26ZrF8uORgBRHHmPLSNccMk62fgvoFfBp7Hvfugfj9YdzHbllnazaXa2PzrDYyaEiOG_IBaUSyxiD378C1z3x2HnTRhCI8llzE_XXo_QcuidG2xn9sNq2ww_DQZzysqMWRns0ffnfsfF1ra_wXM4Hng3Amt36IdfekQk4yC8_GGUu6Y3zY9h5cxDRQBTwECB8D-sLVZ9v7P_fsoT2RWgPQ</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>TALBOT, NEIL C</creator><creator>CAPERNA, THOMAS J</creator><creator>POWELL, ANNE M</creator><creator>EALY, ALAN D</creator><creator>BLOMBERG, LE ANN</creator><creator>GARRETT, WESLEY M</creator><general>Society for In Vitro Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST</title><author>TALBOT, NEIL C ; 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Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TALBOT, NEIL C</au><au>CAPERNA, THOMAS J</au><au>POWELL, ANNE M</au><au>EALY, ALAN D</au><au>BLOMBERG, LE ANN</au><au>GARRETT, WESLEY M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>41</volume><issue>5</issue><spage>130</spage><epage>141</epage><pages>130-141</pages><issn>1071-2690</issn><issn>1543-706X</issn><eissn>1543-706X</eissn><coden>IVCAED</coden><abstract>A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with desmosomes. Rough endoplasmic reticulum was prominent within the cells as were lipid vacuoles. Immunocytochemistry indicated the BPE-1 cells had robust microtubule networks. These cells have been grown for over 2 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The BPE-1 cell line presumably arose from embryonic cells that became diploid soon after parthenogenetic activation and development of the early embryo. However, metaphase spreads prepared at passage 41 indicated that the cell population had a hypodiploid (2n = 60) unimodal chromosome content with a mode of 53 and a median and mean of 52. The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos.</abstract><cop>Germany</cop><pub>Society for In Vitro Biology</pub><pmid>16153145</pmid><doi>10.1290/040901.1</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Blastocyst Blastocyst - cytology bovine Cattle cell CELL AND TISSUE MODELS cell culture Cell culture techniques Cell Line - cytology Cell Line - ultrastructure Cell lines chromosome number Chromosomes, Mammalian - genetics Cultured cells Cytogenetic Analysis Diploidy Embryos Endoderm Endoderm - cytology Feeder cells Immunohistochemistry Interferon Type I line Microscopy, Electron, Transmission Parthenogenesis parthenogenic Pregnancy Proteins rough endoplasmic reticulum T lymphocytes tight junctions Ungulates vacuoles visceral endoderm cell lines |
| title | ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST |
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