Capillary electrophoresis for the analysis of glycoprotein pharmaceuticals
Carbohydrate chains in glycoprotein pharmaceuticals play important roles for the expression of their biological activities, but the structure and compositions of carbohydrate chains are dependent on the conditions for their production. Therefore, evaluation of the carbohydrate chains is quite import...
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| Veröffentlicht in: | Electrophoresis 2006-06, Vol.27 (12), p.2495-2504 |
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| creator | Kamoda, Satoru Kakehi, Kazuaki |
| description | Carbohydrate chains in glycoprotein pharmaceuticals play important roles for the expression of their biological activities, but the structure and compositions of carbohydrate chains are dependent on the conditions for their production. Therefore, evaluation of the carbohydrate chains is quite important for productive process development, characterization of product for approval application, and routine quality control. The oligosaccharides themselves have complex structure including blanching and various glycosidic linkages, and oligosaccharides in one glycoprotein pharmaceutical generally have high heterogeneity, and characterization of oligosaccharide moiety in glycoprotein has been a challenging target. In these situations, CE has been realized as a powerful tool for oligosaccharide analysis due to its high resolution and automatic operating system. This review focuses on the application of CE to the glycoform analysis of glycoproteins and profiling of the N‐linked glycans released from glycoprotein pharmaceuticals. Current applications for structure analysis using CE‐MSn technique and glycan profiling method for therapeutic antibody are also described. |
| doi_str_mv | 10.1002/elps.200500853 |
| format | Article |
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Therefore, evaluation of the carbohydrate chains is quite important for productive process development, characterization of product for approval application, and routine quality control. The oligosaccharides themselves have complex structure including blanching and various glycosidic linkages, and oligosaccharides in one glycoprotein pharmaceutical generally have high heterogeneity, and characterization of oligosaccharide moiety in glycoprotein has been a challenging target. In these situations, CE has been realized as a powerful tool for oligosaccharide analysis due to its high resolution and automatic operating system. This review focuses on the application of CE to the glycoform analysis of glycoproteins and profiling of the N‐linked glycans released from glycoprotein pharmaceuticals. 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Current applications for structure analysis using CE‐MSn technique and glycan profiling method for therapeutic antibody are also described.</description><subject>Capillary electrophoresis</subject><subject>Carbohydrate Sequence</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Glycoforms</subject><subject>Glycoprotein pharmaceuticals</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - chemistry</subject><subject>Mass Spectrometry</subject><subject>N-Linked oligosaccharides</subject><subject>Oligosaccharides - analysis</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Polysaccharides - analysis</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAUxC0EglJYGVEmthQ7_swIpRSqChAfqsRiOe4LDaRNsBNB_3tctSpsTCc9_e507xA6IbhHME7Ooax9L8GYY6w43UEdwpMkToSiu6iDiaQxVpQfoEPv3zHGLGVsHx0QIYkSjHbQqG_qoiyNW0ZQgm1cVc8qB77wUV65qJlBZBamXK4OVR69lUtb1a5qoFhE9cy4ubHQNoU1pT9Ce3kQON5oF71cD577N_H4fnjbvxjHlrHQh1qTTCEDkcqUcmMFVVbkKZ8amDJDpJWCARMZV6nlmbGE2ZxIztMsMCrNaBedrXNDj88WfKPnhbcQnlhA1XotFJdCJTSAvTVoXeW9g1zXrpiHVzXBerWeXq2nt-sFw-kmuc3mMP3FN3MFIF0DX0UJy3_i9GD88PQ3PF57C9_A99Zr3IcWkkquJ3dDPbx6FJfD0aue0B96So0y</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Kamoda, Satoru</creator><creator>Kakehi, Kazuaki</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Capillary electrophoresis for the analysis of glycoprotein pharmaceuticals</title><author>Kamoda, Satoru ; Kakehi, Kazuaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4473-3ca2debe697935ac638c6f95daed4a17c764e46b589c5bac14cf17559b95d89b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Capillary electrophoresis</topic><topic>Carbohydrate Sequence</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Glycoforms</topic><topic>Glycoprotein pharmaceuticals</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - chemistry</topic><topic>Mass Spectrometry</topic><topic>N-Linked oligosaccharides</topic><topic>Oligosaccharides - analysis</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Polysaccharides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamoda, Satoru</creatorcontrib><creatorcontrib>Kakehi, Kazuaki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamoda, Satoru</au><au>Kakehi, Kazuaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary electrophoresis for the analysis of glycoprotein pharmaceuticals</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>27</volume><issue>12</issue><spage>2495</spage><epage>2504</epage><pages>2495-2504</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Carbohydrate chains in glycoprotein pharmaceuticals play important roles for the expression of their biological activities, but the structure and compositions of carbohydrate chains are dependent on the conditions for their production. 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| ispartof | Electrophoresis, 2006-06, Vol.27 (12), p.2495-2504 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Capillary electrophoresis Carbohydrate Sequence Electrophoresis, Capillary - methods Glycoforms Glycoprotein pharmaceuticals Glycoproteins - analysis Glycoproteins - chemistry Mass Spectrometry N-Linked oligosaccharides Oligosaccharides - analysis Pharmaceutical Preparations - analysis Polysaccharides - analysis |
| title | Capillary electrophoresis for the analysis of glycoprotein pharmaceuticals |
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