In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes
Water and 80% ethanol extracts of 20 Thai medicinal plants used to treat AIDS were tested for their HIV type 1 reverse transcriptase inhibitory activity. The water extracts of Ipomoea carnea subsp. fistulosa (aerial parts), Vitex glabrata (branch), Vitex trifolia (aerial part), Vitex negundo (aerial...
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| Veröffentlicht in: | Journal of ethnopharmacology 2005-10, Vol.101 (1), p.84-89 |
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| creator | Woradulayapinij, Warunya Soonthornchareonnon, Noppamas Wiwat, Chanpen |
| description | Water and 80% ethanol extracts of 20 Thai medicinal plants used to treat AIDS were tested for their HIV type 1 reverse transcriptase inhibitory activity. The water extracts of
Ipomoea carnea subsp.
fistulosa (aerial parts),
Vitex glabrata (branch),
Vitex trifolia (aerial part),
Vitex negundo (aerial part),
Canna indica (rhizome), and
Justicia gendarussa (aerial part) showed HIV-1 RT inhibition ratio (% IR) higher than 90% at a 200
μg/ml concentration. The water extract of
Canna indica rhizomes (IC
50 22.56
μg/ml) was selected for further study, i.e. for its HIV-1 RT inhibition activity and the purification and characterization of the active proteins. Proteins in water extract were fractionated by ammonium sulfate precipitation and separated by sodium dodecyl sulfate acrylamide gel electrophoresis (SDS-PAGE), yielding two proteins, Cip31 (31
kDa) and Cip14 (14
kDa) with IC
50 of 17.41 and 19.25
μg/ml and isoelectric point (p
I) of 3.5 and 6.35, respectively. Both proteins showed significant HIV-1 RT inhibition. |
| doi_str_mv | 10.1016/j.jep.2005.03.030 |
| format | Article |
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Ipomoea carnea subsp.
fistulosa (aerial parts),
Vitex glabrata (branch),
Vitex trifolia (aerial part),
Vitex negundo (aerial part),
Canna indica (rhizome), and
Justicia gendarussa (aerial part) showed HIV-1 RT inhibition ratio (% IR) higher than 90% at a 200
μg/ml concentration. The water extract of
Canna indica rhizomes (IC
50 22.56
μg/ml) was selected for further study, i.e. for its HIV-1 RT inhibition activity and the purification and characterization of the active proteins. Proteins in water extract were fractionated by ammonium sulfate precipitation and separated by sodium dodecyl sulfate acrylamide gel electrophoresis (SDS-PAGE), yielding two proteins, Cip31 (31
kDa) and Cip14 (14
kDa) with IC
50 of 17.41 and 19.25
μg/ml and isoelectric point (p
I) of 3.5 and 6.35, respectively. Both proteins showed significant HIV-1 RT inhibition.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2005.03.030</identifier><identifier>PMID: 15951145</identifier><identifier>CODEN: JOETD7</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Anti-HIV-1 ; Biological and medical sciences ; Canna indica ; Canna indica L ; Cannaceae ; chemical constituents of plants ; enzyme inhibitors ; ethnobotany ; General pharmacology ; herbal medicines ; HIV infections ; HIV Reverse Transcriptase - antagonists & inhibitors ; HIV-1 reverse transcriptase inhibitor ; Ipomoea carnea ; Justicia ; Justicia gendarussa ; Medical sciences ; medicinal plants ; medicinal properties ; Molecular Weight ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; plant extracts ; Plant Extracts - pharmacology ; Plant Proteins - pharmacology ; Plants, Medicinal ; Reverse Transcriptase Inhibitors - pharmacology ; Rhizome - chemistry ; rhizomes ; RNA-directed DNA polymerase ; Thailand ; traditional medicine ; Vitex ; Vitex glabrata ; Vitex negundo ; Vitex trifolia</subject><ispartof>Journal of ethnopharmacology, 2005-10, Vol.101 (1), p.84-89</ispartof><rights>2005 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-964f6732b361e29b631ce33867718e96f70694d641c232e188b57fd099ef8d1f3</citedby><cites>FETCH-LOGICAL-c437t-964f6732b361e29b631ce33867718e96f70694d641c232e188b57fd099ef8d1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874105002886$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17117053$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15951145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woradulayapinij, Warunya</creatorcontrib><creatorcontrib>Soonthornchareonnon, Noppamas</creatorcontrib><creatorcontrib>Wiwat, Chanpen</creatorcontrib><title>In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Water and 80% ethanol extracts of 20 Thai medicinal plants used to treat AIDS were tested for their HIV type 1 reverse transcriptase inhibitory activity. The water extracts of
Ipomoea carnea subsp.
fistulosa (aerial parts),
Vitex glabrata (branch),
Vitex trifolia (aerial part),
Vitex negundo (aerial part),
Canna indica (rhizome), and
Justicia gendarussa (aerial part) showed HIV-1 RT inhibition ratio (% IR) higher than 90% at a 200
μg/ml concentration. The water extract of
Canna indica rhizomes (IC
50 22.56
μg/ml) was selected for further study, i.e. for its HIV-1 RT inhibition activity and the purification and characterization of the active proteins. Proteins in water extract were fractionated by ammonium sulfate precipitation and separated by sodium dodecyl sulfate acrylamide gel electrophoresis (SDS-PAGE), yielding two proteins, Cip31 (31
kDa) and Cip14 (14
kDa) with IC
50 of 17.41 and 19.25
μg/ml and isoelectric point (p
I) of 3.5 and 6.35, respectively. Both proteins showed significant HIV-1 RT inhibition.</description><subject>Anti-HIV-1</subject><subject>Biological and medical sciences</subject><subject>Canna indica</subject><subject>Canna indica L</subject><subject>Cannaceae</subject><subject>chemical constituents of plants</subject><subject>enzyme inhibitors</subject><subject>ethnobotany</subject><subject>General pharmacology</subject><subject>herbal medicines</subject><subject>HIV infections</subject><subject>HIV Reverse Transcriptase - antagonists & inhibitors</subject><subject>HIV-1 reverse transcriptase inhibitor</subject><subject>Ipomoea carnea</subject><subject>Justicia</subject><subject>Justicia gendarussa</subject><subject>Medical sciences</subject><subject>medicinal plants</subject><subject>medicinal properties</subject><subject>Molecular Weight</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Proteins - pharmacology</subject><subject>Plants, Medicinal</subject><subject>Reverse Transcriptase Inhibitors - pharmacology</subject><subject>Rhizome - chemistry</subject><subject>rhizomes</subject><subject>RNA-directed DNA polymerase</subject><subject>Thailand</subject><subject>traditional medicine</subject><subject>Vitex</subject><subject>Vitex glabrata</subject><subject>Vitex negundo</subject><subject>Vitex trifolia</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGP0zAQhSMEYsvCD-ACvgCnhpk4sR1xQhWwlSpxYJer5Thj6ipNgu1WKr8eV620t5VGsix_73n0XlG8RSgRUHzelTuaywqgKYHngWfFApWslrKR_HmxAC7VUskab4pXMe4AQGINL4sbbNoGsW4WxWE9sqNPYWJ3698snWZiyAIdKURiKZgx2uDnZPLNj1vf-TSFEzM2-azyFNnk2P3WeLan3ls_moHNgxlTZGbs2cqMo8nC_GTYpmRh6_9Ne4qvixfODJHeXM_b4uH7t_vV3XLz88d69XWztDWXadmK2gnJq44LpKrtBEdLnCshJSpqhZMg2roXNdqKV4RKdY10PbQtOdWj47fFp4vvHKa_B4pJ7320NOQNaTpEnX1Em73aTH58khSqkTVvziBeQBumGAM5PQe_N-GkEfS5Fb3TuRV9bkUDzwNZ8-5qfuhyTo-Kaw0Z-HAFTLRmcDl36-MjJxElNDxz7y-cM5M2f0JmHn5VgBwQeA3q_NWXC0E51qOnoKP1NNrcTiCbdD_5Jxb9DwBhsks</recordid><startdate>20051003</startdate><enddate>20051003</enddate><creator>Woradulayapinij, Warunya</creator><creator>Soonthornchareonnon, Noppamas</creator><creator>Wiwat, Chanpen</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051003</creationdate><title>In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes</title><author>Woradulayapinij, Warunya ; Soonthornchareonnon, Noppamas ; Wiwat, Chanpen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-964f6732b361e29b631ce33867718e96f70694d641c232e188b57fd099ef8d1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Anti-HIV-1</topic><topic>Biological and medical sciences</topic><topic>Canna indica</topic><topic>Canna indica L</topic><topic>Cannaceae</topic><topic>chemical constituents of plants</topic><topic>enzyme inhibitors</topic><topic>ethnobotany</topic><topic>General pharmacology</topic><topic>herbal medicines</topic><topic>HIV infections</topic><topic>HIV Reverse Transcriptase - antagonists & inhibitors</topic><topic>HIV-1 reverse transcriptase inhibitor</topic><topic>Ipomoea carnea</topic><topic>Justicia</topic><topic>Justicia gendarussa</topic><topic>Medical sciences</topic><topic>medicinal plants</topic><topic>medicinal properties</topic><topic>Molecular Weight</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>plant extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Proteins - pharmacology</topic><topic>Plants, Medicinal</topic><topic>Reverse Transcriptase Inhibitors - pharmacology</topic><topic>Rhizome - chemistry</topic><topic>rhizomes</topic><topic>RNA-directed DNA polymerase</topic><topic>Thailand</topic><topic>traditional medicine</topic><topic>Vitex</topic><topic>Vitex glabrata</topic><topic>Vitex negundo</topic><topic>Vitex trifolia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woradulayapinij, Warunya</creatorcontrib><creatorcontrib>Soonthornchareonnon, Noppamas</creatorcontrib><creatorcontrib>Wiwat, Chanpen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woradulayapinij, Warunya</au><au>Soonthornchareonnon, Noppamas</au><au>Wiwat, Chanpen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2005-10-03</date><risdate>2005</risdate><volume>101</volume><issue>1</issue><spage>84</spage><epage>89</epage><pages>84-89</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><coden>JOETD7</coden><abstract>Water and 80% ethanol extracts of 20 Thai medicinal plants used to treat AIDS were tested for their HIV type 1 reverse transcriptase inhibitory activity. The water extracts of
Ipomoea carnea subsp.
fistulosa (aerial parts),
Vitex glabrata (branch),
Vitex trifolia (aerial part),
Vitex negundo (aerial part),
Canna indica (rhizome), and
Justicia gendarussa (aerial part) showed HIV-1 RT inhibition ratio (% IR) higher than 90% at a 200
μg/ml concentration. The water extract of
Canna indica rhizomes (IC
50 22.56
μg/ml) was selected for further study, i.e. for its HIV-1 RT inhibition activity and the purification and characterization of the active proteins. Proteins in water extract were fractionated by ammonium sulfate precipitation and separated by sodium dodecyl sulfate acrylamide gel electrophoresis (SDS-PAGE), yielding two proteins, Cip31 (31
kDa) and Cip14 (14
kDa) with IC
50 of 17.41 and 19.25
μg/ml and isoelectric point (p
I) of 3.5 and 6.35, respectively. Both proteins showed significant HIV-1 RT inhibition.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15951145</pmid><doi>10.1016/j.jep.2005.03.030</doi><tpages>6</tpages></addata></record> |
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| issn | 0378-8741 1872-7573 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-HIV-1 Biological and medical sciences Canna indica Canna indica L Cannaceae chemical constituents of plants enzyme inhibitors ethnobotany General pharmacology herbal medicines HIV infections HIV Reverse Transcriptase - antagonists & inhibitors HIV-1 reverse transcriptase inhibitor Ipomoea carnea Justicia Justicia gendarussa Medical sciences medicinal plants medicinal properties Molecular Weight Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments plant extracts Plant Extracts - pharmacology Plant Proteins - pharmacology Plants, Medicinal Reverse Transcriptase Inhibitors - pharmacology Rhizome - chemistry rhizomes RNA-directed DNA polymerase Thailand traditional medicine Vitex Vitex glabrata Vitex negundo Vitex trifolia |
| title | In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes |
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