Functional Coupling of Cleavage and Polyadenylation with Transcription of mRNA

Cleavage and polyadenylation define the 3′ ends of almost all eukaryotic mRNAs and are thought to occur during transcription. We describe a human in vitro system utilizing an immobilized template, in which transcripts in RNA polymerase II elongation complexes are efficiently cleaved and polyadenylat...

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Veröffentlicht in:	The Journal of biological chemistry 2005-09, Vol.280 (37), p.32262-32271
Hauptverfasser:	Adamson, Todd E., Shutt, Damon C., Price, David H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - chemistry Blotting, Western Cell Nucleus - metabolism Chymotrypsin - chemistry Chymotrypsin - pharmacology DNA - chemistry DNA, Complementary - metabolism HeLa Cells Humans Macromolecular Substances - chemistry Poly A - chemistry Polyadenylation Polymerase Chain Reaction Positive Transcriptional Elongation Factor B - metabolism Potassium Chloride - chemistry Protein Structure, Tertiary RNA - chemistry RNA Polymerase II - chemistry RNA, Messenger - metabolism Salts - pharmacology Time Factors Transcription, Genetic
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