Functional Assays for Replication Protein A (RPA)

Replication protein A (RPA) is a heterotrimeric, single‐stranded DNA‐binding protein. RPA is conserved in all eukaryotes and is essential for DNA replication, DNA repair, and recombination. RPA also plays a role in coordinating DNA metabolism and the cellular response to DNA damage. Assays have been...

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Veröffentlicht in:	Methods in Enzymology 2006, Vol.409, p.11-38
Hauptverfasser:	Binz, Sara K., Dickson, Anne M., Haring, Stuart J., Wold, Marc S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity DNA Repair DNA Replication Enzyme-Linked Immunosorbent Assay Fluorescence Polarization Fluorescent Antibody Technique, Indirect HeLa Cells Humans Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Replication Protein A - isolation & purification Replication Protein A - physiology Surface Plasmon Resonance
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