Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences
The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed Babesia species (designated B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated...
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| Veröffentlicht in: | Veterinary parasitology 2005-10, Vol.133 (1), p.1-6 |
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| creator | Luo, Jianxun Yin, Hong Liu, Zhijie Yang, Dongying Guan, Guiquan Liu, Aihong Ma, Miling Dang, Shengzhi Lu, Bingyi Sun, Caiqin Bai, Qi Lu, Wenshun Chen, Puyan |
| description | The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed
Babesia species (designated
B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other
Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated to the pGEM-T Easy vector for sequencing. It was found that the length of the 18S rRNA gene of all
B. U sp. Kashi 1 and
B. U sp. Kashi 2 was 1699
bp and 1689
bp. Two phylogenetic trees were, respectively, inferred based on 18S rRNA sequence of the Chinese bovine
Babesia isolates and all of
Babesia species available in GenBank. The first tree showed that
B. U sp. was situated in the branch between
B. major Yili and
B. bovis Shannxian, and the second tree revealed that
B. U sp. was confined to the same group as
B. caballi. The percent identity of
B. U sp. with other Chinese
Babesia species was between 74.2 and 91.8, while the percent identity between two
B. U sp. isolates was 99.7. These results demonstrated that this
B. U sp. is different from other
Babesia species, but that two
B. U sp. isolates obtained with nymphal and adultal
Hyalomma anatolicum anatolicum tick belong to the same species. |
| doi_str_mv | 10.1016/j.vetpar.2005.02.014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68559095</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304401705002414</els_id><sourcerecordid>17064566</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-d9d002932f0be524eed357b0b6ff6253230617fbe3550b05ab9a5ea918bb65f73</originalsourceid><addsrcrecordid>eNqFkU2P1SAUhonRONfRf2CUlbvWAxTabkxmJn4loybqrAm0pyM3vaVy2pvMv5eb3sSdrkjgOS_wPoy9FFAKEObtvjziMrtUSgBdgixBVI_YTjS1KqTW8JjtQEFVVCDqC_aMaA8AFZj6KbsQum1kI8WO0Zc4YreOLvH518MY73HCJXSclrUPSDxO3E18nSZ3wJ77eAwT8mvnkYLjNJfcO8oHGaODG0dOq1-nsPAUfKSYt_j3r1f8lMoJf684dUjP2ZPBjYQvzuslu_vw_ufNp-L228fPN1e3RVcJvRR92wPIVskBPGpZIfZK1x68GQYjtZIKjKgHjyr_1oN2vnUaXSsa740eanXJ3my5c4r5alrsIVCH4-gmjCtZ02jdQqv_C4oaTKWNyWC1gV2KRAkHO6dwcOnBCrAnK3ZvNyv2ZMWCtNlKHnt1zl99rvHv0FlDBl5vwOCidfcpkL37IUEoEKBE06hMvNsIzIUdAyZLXTi12YeE3WL7GP79hj-4nKn0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17064566</pqid></control><display><type>article</type><title>Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Luo, Jianxun ; Yin, Hong ; Liu, Zhijie ; Yang, Dongying ; Guan, Guiquan ; Liu, Aihong ; Ma, Miling ; Dang, Shengzhi ; Lu, Bingyi ; Sun, Caiqin ; Bai, Qi ; Lu, Wenshun ; Chen, Puyan</creator><creatorcontrib>Luo, Jianxun ; Yin, Hong ; Liu, Zhijie ; Yang, Dongying ; Guan, Guiquan ; Liu, Aihong ; Ma, Miling ; Dang, Shengzhi ; Lu, Bingyi ; Sun, Caiqin ; Bai, Qi ; Lu, Wenshun ; Chen, Puyan</creatorcontrib><description>The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed
Babesia species (designated
B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other
Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated to the pGEM-T Easy vector for sequencing. It was found that the length of the 18S rRNA gene of all
B. U sp. Kashi 1 and
B. U sp. Kashi 2 was 1699
bp and 1689
bp. Two phylogenetic trees were, respectively, inferred based on 18S rRNA sequence of the Chinese bovine
Babesia isolates and all of
Babesia species available in GenBank. The first tree showed that
B. U sp. was situated in the branch between
B. major Yili and
B. bovis Shannxian, and the second tree revealed that
B. U sp. was confined to the same group as
B. caballi. The percent identity of
B. U sp. with other Chinese
Babesia species was between 74.2 and 91.8, while the percent identity between two
B. U sp. isolates was 99.7. These results demonstrated that this
B. U sp. is different from other
Babesia species, but that two
B. U sp. isolates obtained with nymphal and adultal
Hyalomma anatolicum anatolicum tick belong to the same species.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2005.02.014</identifier><identifier>PMID: 15982821</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>18S rRNA ; Animals ; Babesia ; Babesia - genetics ; Babesia U sp ; babesiosis ; Babesiosis - parasitology ; Babesiosis - veterinary ; Base Sequence ; calves ; Cattle ; Cattle Diseases - parasitology ; China ; disease vectors ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Hyalomma anatolicum anatolicum ; Ixodidae ; Molecular Sequence Data ; molecular systematics ; nucleotide sequences ; Phylogenetic relationship ; Phylogeny ; Polymerase Chain Reaction - veterinary ; ribosomal DNA ; RNA, Ribosomal, 18S - chemistry ; RNA, Ribosomal, 18S - genetics ; Sequence Alignment ; sequence homology ; ticks</subject><ispartof>Veterinary parasitology, 2005-10, Vol.133 (1), p.1-6</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-d9d002932f0be524eed357b0b6ff6253230617fbe3550b05ab9a5ea918bb65f73</citedby><cites>FETCH-LOGICAL-c415t-d9d002932f0be524eed357b0b6ff6253230617fbe3550b05ab9a5ea918bb65f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401705002414$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15982821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Jianxun</creatorcontrib><creatorcontrib>Yin, Hong</creatorcontrib><creatorcontrib>Liu, Zhijie</creatorcontrib><creatorcontrib>Yang, Dongying</creatorcontrib><creatorcontrib>Guan, Guiquan</creatorcontrib><creatorcontrib>Liu, Aihong</creatorcontrib><creatorcontrib>Ma, Miling</creatorcontrib><creatorcontrib>Dang, Shengzhi</creatorcontrib><creatorcontrib>Lu, Bingyi</creatorcontrib><creatorcontrib>Sun, Caiqin</creatorcontrib><creatorcontrib>Bai, Qi</creatorcontrib><creatorcontrib>Lu, Wenshun</creatorcontrib><creatorcontrib>Chen, Puyan</creatorcontrib><title>Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed
Babesia species (designated
B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other
Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated to the pGEM-T Easy vector for sequencing. It was found that the length of the 18S rRNA gene of all
B. U sp. Kashi 1 and
B. U sp. Kashi 2 was 1699
bp and 1689
bp. Two phylogenetic trees were, respectively, inferred based on 18S rRNA sequence of the Chinese bovine
Babesia isolates and all of
Babesia species available in GenBank. The first tree showed that
B. U sp. was situated in the branch between
B. major Yili and
B. bovis Shannxian, and the second tree revealed that
B. U sp. was confined to the same group as
B. caballi. The percent identity of
B. U sp. with other Chinese
Babesia species was between 74.2 and 91.8, while the percent identity between two
B. U sp. isolates was 99.7. These results demonstrated that this
B. U sp. is different from other
Babesia species, but that two
B. U sp. isolates obtained with nymphal and adultal
Hyalomma anatolicum anatolicum tick belong to the same species.</description><subject>18S rRNA</subject><subject>Animals</subject><subject>Babesia</subject><subject>Babesia - genetics</subject><subject>Babesia U sp</subject><subject>babesiosis</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - veterinary</subject><subject>Base Sequence</subject><subject>calves</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>China</subject><subject>disease vectors</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Hyalomma anatolicum anatolicum</subject><subject>Ixodidae</subject><subject>Molecular Sequence Data</subject><subject>molecular systematics</subject><subject>nucleotide sequences</subject><subject>Phylogenetic relationship</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>ribosomal DNA</subject><subject>RNA, Ribosomal, 18S - chemistry</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Sequence Alignment</subject><subject>sequence homology</subject><subject>ticks</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1SAUhonRONfRf2CUlbvWAxTabkxmJn4loybqrAm0pyM3vaVy2pvMv5eb3sSdrkjgOS_wPoy9FFAKEObtvjziMrtUSgBdgixBVI_YTjS1KqTW8JjtQEFVVCDqC_aMaA8AFZj6KbsQum1kI8WO0Zc4YreOLvH518MY73HCJXSclrUPSDxO3E18nSZ3wJ77eAwT8mvnkYLjNJfcO8oHGaODG0dOq1-nsPAUfKSYt_j3r1f8lMoJf684dUjP2ZPBjYQvzuslu_vw_ufNp-L228fPN1e3RVcJvRR92wPIVskBPGpZIfZK1x68GQYjtZIKjKgHjyr_1oN2vnUaXSsa740eanXJ3my5c4r5alrsIVCH4-gmjCtZ02jdQqv_C4oaTKWNyWC1gV2KRAkHO6dwcOnBCrAnK3ZvNyv2ZMWCtNlKHnt1zl99rvHv0FlDBl5vwOCidfcpkL37IUEoEKBE06hMvNsIzIUdAyZLXTi12YeE3WL7GP79hj-4nKn0</recordid><startdate>20051010</startdate><enddate>20051010</enddate><creator>Luo, Jianxun</creator><creator>Yin, Hong</creator><creator>Liu, Zhijie</creator><creator>Yang, Dongying</creator><creator>Guan, Guiquan</creator><creator>Liu, Aihong</creator><creator>Ma, Miling</creator><creator>Dang, Shengzhi</creator><creator>Lu, Bingyi</creator><creator>Sun, Caiqin</creator><creator>Bai, Qi</creator><creator>Lu, Wenshun</creator><creator>Chen, Puyan</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051010</creationdate><title>Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences</title><author>Luo, Jianxun ; Yin, Hong ; Liu, Zhijie ; Yang, Dongying ; Guan, Guiquan ; Liu, Aihong ; Ma, Miling ; Dang, Shengzhi ; Lu, Bingyi ; Sun, Caiqin ; Bai, Qi ; Lu, Wenshun ; Chen, Puyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-d9d002932f0be524eed357b0b6ff6253230617fbe3550b05ab9a5ea918bb65f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>18S rRNA</topic><topic>Animals</topic><topic>Babesia</topic><topic>Babesia - genetics</topic><topic>Babesia U sp</topic><topic>babesiosis</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - veterinary</topic><topic>Base Sequence</topic><topic>calves</topic><topic>Cattle</topic><topic>Cattle Diseases - parasitology</topic><topic>China</topic><topic>disease vectors</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Hyalomma anatolicum anatolicum</topic><topic>Ixodidae</topic><topic>Molecular Sequence Data</topic><topic>molecular systematics</topic><topic>nucleotide sequences</topic><topic>Phylogenetic relationship</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>ribosomal DNA</topic><topic>RNA, Ribosomal, 18S - chemistry</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Sequence Alignment</topic><topic>sequence homology</topic><topic>ticks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jianxun</creatorcontrib><creatorcontrib>Yin, Hong</creatorcontrib><creatorcontrib>Liu, Zhijie</creatorcontrib><creatorcontrib>Yang, Dongying</creatorcontrib><creatorcontrib>Guan, Guiquan</creatorcontrib><creatorcontrib>Liu, Aihong</creatorcontrib><creatorcontrib>Ma, Miling</creatorcontrib><creatorcontrib>Dang, Shengzhi</creatorcontrib><creatorcontrib>Lu, Bingyi</creatorcontrib><creatorcontrib>Sun, Caiqin</creatorcontrib><creatorcontrib>Bai, Qi</creatorcontrib><creatorcontrib>Lu, Wenshun</creatorcontrib><creatorcontrib>Chen, Puyan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jianxun</au><au>Yin, Hong</au><au>Liu, Zhijie</au><au>Yang, Dongying</au><au>Guan, Guiquan</au><au>Liu, Aihong</au><au>Ma, Miling</au><au>Dang, Shengzhi</au><au>Lu, Bingyi</au><au>Sun, Caiqin</au><au>Bai, Qi</au><au>Lu, Wenshun</au><au>Chen, Puyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2005-10-10</date><risdate>2005</risdate><volume>133</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed
Babesia species (designated
B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other
Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated to the pGEM-T Easy vector for sequencing. It was found that the length of the 18S rRNA gene of all
B. U sp. Kashi 1 and
B. U sp. Kashi 2 was 1699
bp and 1689
bp. Two phylogenetic trees were, respectively, inferred based on 18S rRNA sequence of the Chinese bovine
Babesia isolates and all of
Babesia species available in GenBank. The first tree showed that
B. U sp. was situated in the branch between
B. major Yili and
B. bovis Shannxian, and the second tree revealed that
B. U sp. was confined to the same group as
B. caballi. The percent identity of
B. U sp. with other Chinese
Babesia species was between 74.2 and 91.8, while the percent identity between two
B. U sp. isolates was 99.7. These results demonstrated that this
B. U sp. is different from other
Babesia species, but that two
B. U sp. isolates obtained with nymphal and adultal
Hyalomma anatolicum anatolicum tick belong to the same species.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15982821</pmid><doi>10.1016/j.vetpar.2005.02.014</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary parasitology, 2005-10, Vol.133 (1), p.1-6 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68559095 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 18S rRNA Animals Babesia Babesia - genetics Babesia U sp babesiosis Babesiosis - parasitology Babesiosis - veterinary Base Sequence calves Cattle Cattle Diseases - parasitology China disease vectors DNA, Protozoan - chemistry DNA, Protozoan - genetics Hyalomma anatolicum anatolicum Ixodidae Molecular Sequence Data molecular systematics nucleotide sequences Phylogenetic relationship Phylogeny Polymerase Chain Reaction - veterinary ribosomal DNA RNA, Ribosomal, 18S - chemistry RNA, Ribosomal, 18S - genetics Sequence Alignment sequence homology ticks |
| title | Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences |
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