Immunolocalization of inhibin/activin subunits in the Shiba goat fetal, neonatal, and adult testes
The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (beta A and beta B) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (...
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| Veröffentlicht in: | Journal of Reproduction and Development 2005, Vol.51(4), pp.521-526 |
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| container_title | Journal of Reproduction and Development |
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| creator | Weng, Q.(Beijing Forestry Univ. (China)) Medan, M.S Ren, L.Q Watanabe, G Arai, K.Y Taya, K |
| description | The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (beta A and beta B) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (both of 3 years old). The sections of testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin alpha, inhibin/activin beta A, and inhibin/achvin beta B. Inhibin alpha and inhibin/activin (beta A and beta B) subunits were expressed in Leydig cells, but not in the Sertoli cells of the fetus with a weak immunostaining. An increase in the number of positive cells and a more intense immunohistochemical signal for inhibin alpha and inhibin/activin (beta A and beta B) subunits were observed in the Leydig cells of neonatal testes. Moreover, inhibin alpha, beta A, and beta B subunits were expressed in the Sertoli cells and Leydig cells of adult testes, respectively. These results suggest that Shiba goats testes have the ability to synthesize inhibins in the fetus, neonate, and adult, and the cellular localization of inhibin/activin subunits showed age-related changes in fetal, neonatal, and adult testes of Shiba goats. |
| doi_str_mv | 10.1262/jrd.17007 |
| format | Article |
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(China)) ; Medan, M.S ; Ren, L.Q ; Watanabe, G ; Arai, K.Y ; Taya, K</creator><creatorcontrib>Weng, Q.(Beijing Forestry Univ. (China)) ; Medan, M.S ; Ren, L.Q ; Watanabe, G ; Arai, K.Y ; Taya, K</creatorcontrib><description>The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (beta A and beta B) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (both of 3 years old). The sections of testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin alpha, inhibin/activin beta A, and inhibin/achvin beta B. Inhibin alpha and inhibin/activin (beta A and beta B) subunits were expressed in Leydig cells, but not in the Sertoli cells of the fetus with a weak immunostaining. An increase in the number of positive cells and a more intense immunohistochemical signal for inhibin alpha and inhibin/activin (beta A and beta B) subunits were observed in the Leydig cells of neonatal testes. Moreover, inhibin alpha, beta A, and beta B subunits were expressed in the Sertoli cells and Leydig cells of adult testes, respectively. These results suggest that Shiba goats testes have the ability to synthesize inhibins in the fetus, neonate, and adult, and the cellular localization of inhibin/activin subunits showed age-related changes in fetal, neonatal, and adult testes of Shiba goats.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.17007</identifier><identifier>PMID: 16141647</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>Activins - chemistry ; ADULTE ; ADULTOS ; ADULTS ; ANIMAL NOUVEAU-NE ; ANIMAL RECIEN NACIDO ; Animals ; CAPRIN ; CAPRINOS ; DISTRIBUCION EN TEJIDOS ; DISTRIBUTION TISSULAIRE ; Female ; FETO ; Fetus ; FOETUS ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; Goat testes ; GOATS ; HORMONAS ; HORMONE ; HORMONES ; Immunohistochemistry ; IMMUNOLOGICAL TECHNIQUES ; Inhibins ; Inhibins - chemistry ; Leydig Cells - metabolism ; Male ; Neotate ; NEWBORN ANIMALS ; Sertoli Cells - metabolism ; Swine ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS ; TESTES ; TESTICULE ; TESTICULOS ; Testis - embryology ; Testis - metabolism ; Testis - pathology ; Time Factors ; TISSUE DISTRIBUTION</subject><ispartof>Journal of Reproduction and Development, 2005, Vol.51(4), pp.521-526</ispartof><rights>2005 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-11b8313984923003f14696b4e35e0a1943a974d0a0654e8810871633b4b5b2483</citedby><cites>FETCH-LOGICAL-c502t-11b8313984923003f14696b4e35e0a1943a974d0a0654e8810871633b4b5b2483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,1877,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16141647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weng, Q.(Beijing Forestry Univ. (China))</creatorcontrib><creatorcontrib>Medan, M.S</creatorcontrib><creatorcontrib>Ren, L.Q</creatorcontrib><creatorcontrib>Watanabe, G</creatorcontrib><creatorcontrib>Arai, K.Y</creatorcontrib><creatorcontrib>Taya, K</creatorcontrib><title>Immunolocalization of inhibin/activin subunits in the Shiba goat fetal, neonatal, and adult testes</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (beta A and beta B) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (both of 3 years old). The sections of testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin alpha, inhibin/activin beta A, and inhibin/achvin beta B. Inhibin alpha and inhibin/activin (beta A and beta B) subunits were expressed in Leydig cells, but not in the Sertoli cells of the fetus with a weak immunostaining. An increase in the number of positive cells and a more intense immunohistochemical signal for inhibin alpha and inhibin/activin (beta A and beta B) subunits were observed in the Leydig cells of neonatal testes. Moreover, inhibin alpha, beta A, and beta B subunits were expressed in the Sertoli cells and Leydig cells of adult testes, respectively. These results suggest that Shiba goats testes have the ability to synthesize inhibins in the fetus, neonate, and adult, and the cellular localization of inhibin/activin subunits showed age-related changes in fetal, neonatal, and adult testes of Shiba goats.</description><subject>Activins - chemistry</subject><subject>ADULTE</subject><subject>ADULTOS</subject><subject>ADULTS</subject><subject>ANIMAL NOUVEAU-NE</subject><subject>ANIMAL RECIEN NACIDO</subject><subject>Animals</subject><subject>CAPRIN</subject><subject>CAPRINOS</subject><subject>DISTRIBUCION EN TEJIDOS</subject><subject>DISTRIBUTION TISSULAIRE</subject><subject>Female</subject><subject>FETO</subject><subject>Fetus</subject><subject>FOETUS</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>Goat testes</subject><subject>GOATS</subject><subject>HORMONAS</subject><subject>HORMONE</subject><subject>HORMONES</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>Inhibins</subject><subject>Inhibins - chemistry</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Neotate</subject><subject>NEWBORN ANIMALS</subject><subject>Sertoli Cells - metabolism</subject><subject>Swine</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><subject>TESTES</subject><subject>TESTICULE</subject><subject>TESTICULOS</subject><subject>Testis - embryology</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Time Factors</subject><subject>TISSUE DISTRIBUTION</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF1rHCEUhiWkJNskF_kBLV4VCp3EMzqOcxVK6EdKoIW013KccXZdZjRRp9D--prdJQVR4X14PL6EXAK7glrW19s4XEHLWHtEVsCFqoRg7JisWAeyUgrUKXmd0pYxXjdSnJBTkCBAinZFzN08Lz5MocfJ_cXsgqdhpM5vnHH-GvvsfjtP02IW73IqAc0bSx9KjHQdMNPRZpw-UG-Dx90N_UBxWKZMs01lnZNXI07JXhzOM_Lr86eft1-r--9f7m4_3ld9w-pcARjFgXdKdDUvo44gZCeNsLyxDKETHLtWDAyZbIQtv2KqBcm5EaYxtVD8jLzbex9jeFrK03p2qbfThGW2JWmpmka06hl8vwf7GFKKdtSP0c0Y_2hg-rlQXQrVu0IL-_YgXcxsh__kocEC3OyBbcq4ti8Axuz6ye5UDWhx2Gp4SfoNRm19MbzZG0YMGtfRJf3tR82YLCV0UvF_TwSPJw</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Weng, Q.(Beijing Forestry Univ. (China))</creator><creator>Medan, M.S</creator><creator>Ren, L.Q</creator><creator>Watanabe, G</creator><creator>Arai, K.Y</creator><creator>Taya, K</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>Immunolocalization of inhibin/activin subunits in the Shiba goat fetal, neonatal, and adult testes</title><author>Weng, Q.(Beijing Forestry Univ. (China)) ; Medan, M.S ; Ren, L.Q ; Watanabe, G ; Arai, K.Y ; Taya, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-11b8313984923003f14696b4e35e0a1943a974d0a0654e8810871633b4b5b2483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Activins - chemistry</topic><topic>ADULTE</topic><topic>ADULTOS</topic><topic>ADULTS</topic><topic>ANIMAL NOUVEAU-NE</topic><topic>ANIMAL RECIEN NACIDO</topic><topic>Animals</topic><topic>CAPRIN</topic><topic>CAPRINOS</topic><topic>DISTRIBUCION EN TEJIDOS</topic><topic>DISTRIBUTION TISSULAIRE</topic><topic>Female</topic><topic>FETO</topic><topic>Fetus</topic><topic>FOETUS</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>Goat testes</topic><topic>GOATS</topic><topic>HORMONAS</topic><topic>HORMONE</topic><topic>HORMONES</topic><topic>Immunohistochemistry</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>Inhibins</topic><topic>Inhibins - chemistry</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Neotate</topic><topic>NEWBORN ANIMALS</topic><topic>Sertoli Cells - metabolism</topic><topic>Swine</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><topic>TESTES</topic><topic>TESTICULE</topic><topic>TESTICULOS</topic><topic>Testis - embryology</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Time Factors</topic><topic>TISSUE DISTRIBUTION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weng, Q.(Beijing Forestry Univ. (China))</creatorcontrib><creatorcontrib>Medan, M.S</creatorcontrib><creatorcontrib>Ren, L.Q</creatorcontrib><creatorcontrib>Watanabe, G</creatorcontrib><creatorcontrib>Arai, K.Y</creatorcontrib><creatorcontrib>Taya, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weng, Q.(Beijing Forestry Univ. (China))</au><au>Medan, M.S</au><au>Ren, L.Q</au><au>Watanabe, G</au><au>Arai, K.Y</au><au>Taya, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunolocalization of inhibin/activin subunits in the Shiba goat fetal, neonatal, and adult testes</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2005-08-01</date><risdate>2005</risdate><volume>51</volume><issue>4</issue><spage>521</spage><epage>526</epage><pages>521-526</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (beta A and beta B) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (both of 3 years old). The sections of testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin alpha, inhibin/activin beta A, and inhibin/achvin beta B. Inhibin alpha and inhibin/activin (beta A and beta B) subunits were expressed in Leydig cells, but not in the Sertoli cells of the fetus with a weak immunostaining. An increase in the number of positive cells and a more intense immunohistochemical signal for inhibin alpha and inhibin/activin (beta A and beta B) subunits were observed in the Leydig cells of neonatal testes. Moreover, inhibin alpha, beta A, and beta B subunits were expressed in the Sertoli cells and Leydig cells of adult testes, respectively. These results suggest that Shiba goats testes have the ability to synthesize inhibins in the fetus, neonate, and adult, and the cellular localization of inhibin/activin subunits showed age-related changes in fetal, neonatal, and adult testes of Shiba goats.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>16141647</pmid><doi>10.1262/jrd.17007</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Activins - chemistry ADULTE ADULTOS ADULTS ANIMAL NOUVEAU-NE ANIMAL RECIEN NACIDO Animals CAPRIN CAPRINOS DISTRIBUCION EN TEJIDOS DISTRIBUTION TISSULAIRE Female FETO Fetus FOETUS GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS Goat testes GOATS HORMONAS HORMONE HORMONES Immunohistochemistry IMMUNOLOGICAL TECHNIQUES Inhibins Inhibins - chemistry Leydig Cells - metabolism Male Neotate NEWBORN ANIMALS Sertoli Cells - metabolism Swine TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS TESTES TESTICULE TESTICULOS Testis - embryology Testis - metabolism Testis - pathology Time Factors TISSUE DISTRIBUTION |
| title | Immunolocalization of inhibin/activin subunits in the Shiba goat fetal, neonatal, and adult testes |
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