Role of nucleophosmin in embryonic development and tumorigenesis

A role for nucleophosmin Nucleophosmin is an oestrogen-regulated nucleolar protein that has been implicated in a number of cancers including myelodysplastic syndromes (MDS), a group of diseases characterized by overproduction of blood cells in the bone marrow. Now a role for nucleophosmin has been i...

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Veröffentlicht in:	Nature (London) 2005-09, Vol.437 (7055), p.147-153
Hauptverfasser:	Grisendi, Silvia, Bernardi, Rosa, Rossi, Marco, Cheng, Ke, Khandker, Luipa, Manova, Katia, Pandolfi, Pier Paolo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis - genetics Cell Cycle - genetics Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - pathology Cells, Cultured Centrosome - metabolism Embryo Loss - genetics Embryonic Development Embryonic growth stage Embryos Fibroblasts Gene Deletion Genomic Instability - genetics Hematology Hematopoiesis - genetics Humanities and Social Sciences In Situ Hybridization, Fluorescence Inactivation letter Mice Mice, Knockout multidisciplinary Myelodysplastic Syndromes - genetics Myelodysplastic Syndromes - pathology Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology Nuclear Proteins - deficiency Nuclear Proteins - genetics Nuclear Proteins - metabolism Pathogens Prenatal development Science Science (multidisciplinary) Tumors
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description	A role for nucleophosmin Nucleophosmin is an oestrogen-regulated nucleolar protein that has been implicated in a number of cancers including myelodysplastic syndromes (MDS), a group of diseases characterized by overproduction of blood cells in the bone marrow. Now a role for nucleophosmin has been identified in normal cells: it is essential for normal embryonic development and, specifically, for the control of centrosome duplication and genomic stability. Loss of the Npm1 gene in mice produces features similar to those seen in MDS patients. Nucleophosmin (also known as NPM, B23, NO38) is a nucleolar protein directly implicated in cancer pathogenesis, as the NPM1 gene is found mutated and rearranged in a number of haematological disorders 1 , 2 , 3 , 4 , 5 . Furthermore, the region of chromosome 5 to which NPM1 maps is deleted in a proportion of de novo human myelodysplastic syndromes (MDS) 6 , 7 , 8 , 9 , and loss of chromosome 5 is extremely frequent in therapy-related MDS 9 , 10 . NPM is a multifunctional protein 11 , 12 , 13 , 14 , 15 , and its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumour suppressive functions 16 , 17 , 18 , 19 . To study the function of Npm in vivo , we generated a hypomorphic Npm1 mutant series ( Npm1 +/- < Npm1 hy/hy < Npm1 -/- ) in mouse. Here we report that Npm is essential for embryonic development and the maintenance of genomic stability. Npm1 -/- and Npm1 hy/hy mutants have aberrant organogenesis and die between embryonic day E11.5 and E16.5 owing to severe anaemia resulting from defects in primitive haematopoiesis. We show that Npm1 inactivation leads to unrestricted centrosome duplication and genomic instability. We demonstrate that Npm is haploinsufficient in the control of genetic stability and that Npm1 heterozygosity accelerates oncogenesis both in vitro and in vivo . Notably, Npm1 +/- mice develop a haematological syndrome with features of human MDS. Our findings uncover an essential developmental role for Npm and implicate its functional loss in tumorigenesis and MDS pathogenesis.
doi_str_mv	10.1038/nature03915
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Now a role for nucleophosmin has been identified in normal cells: it is essential for normal embryonic development and, specifically, for the control of centrosome duplication and genomic stability. Loss of the Npm1 gene in mice produces features similar to those seen in MDS patients. Nucleophosmin (also known as NPM, B23, NO38) is a nucleolar protein directly implicated in cancer pathogenesis, as the NPM1 gene is found mutated and rearranged in a number of haematological disorders 1 , 2 , 3 , 4 , 5 . Furthermore, the region of chromosome 5 to which NPM1 maps is deleted in a proportion of de novo human myelodysplastic syndromes (MDS) 6 , 7 , 8 , 9 , and loss of chromosome 5 is extremely frequent in therapy-related MDS 9 , 10 . NPM is a multifunctional protein 11 , 12 , 13 , 14 , 15 , and its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumour suppressive functions 16 , 17 , 18 , 19 . To study the function of Npm in vivo , we generated a hypomorphic Npm1 mutant series ( Npm1 +/- < Npm1 hy/hy < Npm1 -/- ) in mouse. Here we report that Npm is essential for embryonic development and the maintenance of genomic stability. Npm1 -/- and Npm1 hy/hy mutants have aberrant organogenesis and die between embryonic day E11.5 and E16.5 owing to severe anaemia resulting from defects in primitive haematopoiesis. We show that Npm1 inactivation leads to unrestricted centrosome duplication and genomic instability. We demonstrate that Npm is haploinsufficient in the control of genetic stability and that Npm1 heterozygosity accelerates oncogenesis both in vitro and in vivo . Notably, Npm1 +/- mice develop a haematological syndrome with features of human MDS. 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Now a role for nucleophosmin has been identified in normal cells: it is essential for normal embryonic development and, specifically, for the control of centrosome duplication and genomic stability. Loss of the Npm1 gene in mice produces features similar to those seen in MDS patients. Nucleophosmin (also known as NPM, B23, NO38) is a nucleolar protein directly implicated in cancer pathogenesis, as the NPM1 gene is found mutated and rearranged in a number of haematological disorders 1 , 2 , 3 , 4 , 5 . Furthermore, the region of chromosome 5 to which NPM1 maps is deleted in a proportion of de novo human myelodysplastic syndromes (MDS) 6 , 7 , 8 , 9 , and loss of chromosome 5 is extremely frequent in therapy-related MDS 9 , 10 . NPM is a multifunctional protein 11 , 12 , 13 , 14 , 15 , and its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumour suppressive functions 16 , 17 , 18 , 19 . 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Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grisendi, Silvia</au><au>Bernardi, Rosa</au><au>Rossi, Marco</au><au>Cheng, Ke</au><au>Khandker, Luipa</au><au>Manova, Katia</au><au>Pandolfi, Pier Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of nucleophosmin in embryonic development and tumorigenesis</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>437</volume><issue>7055</issue><spage>147</spage><epage>153</epage><pages>147-153</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>A role for nucleophosmin Nucleophosmin is an oestrogen-regulated nucleolar protein that has been implicated in a number of cancers including myelodysplastic syndromes (MDS), a group of diseases characterized by overproduction of blood cells in the bone marrow. Now a role for nucleophosmin has been identified in normal cells: it is essential for normal embryonic development and, specifically, for the control of centrosome duplication and genomic stability. Loss of the Npm1 gene in mice produces features similar to those seen in MDS patients. Nucleophosmin (also known as NPM, B23, NO38) is a nucleolar protein directly implicated in cancer pathogenesis, as the NPM1 gene is found mutated and rearranged in a number of haematological disorders 1 , 2 , 3 , 4 , 5 . Furthermore, the region of chromosome 5 to which NPM1 maps is deleted in a proportion of de novo human myelodysplastic syndromes (MDS) 6 , 7 , 8 , 9 , and loss of chromosome 5 is extremely frequent in therapy-related MDS 9 , 10 . NPM is a multifunctional protein 11 , 12 , 13 , 14 , 15 , and its role in oncogenesis is controversial as NPM has been attributed with both oncogenic and tumour suppressive functions 16 , 17 , 18 , 19 . To study the function of Npm in vivo , we generated a hypomorphic Npm1 mutant series ( Npm1 +/- < Npm1 hy/hy < Npm1 -/- ) in mouse. Here we report that Npm is essential for embryonic development and the maintenance of genomic stability. Npm1 -/- and Npm1 hy/hy mutants have aberrant organogenesis and die between embryonic day E11.5 and E16.5 owing to severe anaemia resulting from defects in primitive haematopoiesis. We show that Npm1 inactivation leads to unrestricted centrosome duplication and genomic instability. We demonstrate that Npm is haploinsufficient in the control of genetic stability and that Npm1 heterozygosity accelerates oncogenesis both in vitro and in vivo . Notably, Npm1 +/- mice develop a haematological syndrome with features of human MDS. Our findings uncover an essential developmental role for Npm and implicate its functional loss in tumorigenesis and MDS pathogenesis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>16007073</pmid><doi>10.1038/nature03915</doi><tpages>7</tpages></addata></record>
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subjects	Animals Apoptosis - genetics Cell Cycle - genetics Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - pathology Cells, Cultured Centrosome - metabolism Embryo Loss - genetics Embryonic Development Embryonic growth stage Embryos Fibroblasts Gene Deletion Genomic Instability - genetics Hematology Hematopoiesis - genetics Humanities and Social Sciences In Situ Hybridization, Fluorescence Inactivation letter Mice Mice, Knockout multidisciplinary Myelodysplastic Syndromes - genetics Myelodysplastic Syndromes - pathology Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology Nuclear Proteins - deficiency Nuclear Proteins - genetics Nuclear Proteins - metabolism Pathogens Prenatal development Science Science (multidisciplinary) Tumors
title	Role of nucleophosmin in embryonic development and tumorigenesis
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T18%3A17%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20nucleophosmin%20in%20embryonic%20development%20and%20tumorigenesis&rft.jtitle=Nature%20(London)&rft.au=Grisendi,%20Silvia&rft.date=2005-09-01&rft.volume=437&rft.issue=7055&rft.spage=147&rft.epage=153&rft.pages=147-153&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/nature03915&rft_dat=%3Cgale_proqu%3EA185468885%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204553235&rft_id=info:pmid/16007073&rft_galeid=A185468885&rfr_iscdi=true