Nucleofection of Human Embryonic Stem Cells

Human embryonic stem (hES) cells provide an important tool for the study of human development, disease, and tissue regeneration. Technologies for efficient genetic modification are required to exploit hES cells fully for these applications. Here we present a customized protocol for the transfection...

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Veröffentlicht in:	Stem cells and development 2005-08, Vol.14 (4), p.378-383
Hauptverfasser:	Siemen, Henrike, Nix, Michael, Endl, Elmar, Koch, Philipp, Itskovitz-Eldor, Joseph, Brüstle, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cell Nucleus - metabolism Cell Survival Cells, Cultured DNA - metabolism Electroporation Embryo, Mammalian - cytology Fibroblasts - metabolism Flow Cytometry Genetic Therapy - methods Green Fluorescent Proteins - metabolism Humans Mice Microscopy, Fluorescence Stem Cells - cytology Transfection - methods Transgenes
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