Changes in gas composition and acid-base values of venous blood samples stored under different conditions in 4 domestic species

Background: The effect of storage temperature and time on blood gas and acid‐base values has been investigated intensively in cattle and dogs; however, data are lacking in other species. Objective: The aim of our study was to evaluate changes in gas composition and acid‐base values in venous blood s...

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Veröffentlicht in:Veterinary clinical pathology 2007-12, Vol.36 (4), p.358-360
Hauptverfasser: Piccione, G, Bertolucci, C, Grasso, F, Giudice, E
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Sprache:eng
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Zusammenfassung:Background: The effect of storage temperature and time on blood gas and acid‐base values has been investigated intensively in cattle and dogs; however, data are lacking in other species. Objective: The aim of our study was to evaluate changes in gas composition and acid‐base values in venous blood stored at different temperatures and for different times in 4 domestic species in Italy. Methods: Blood samples from Comisana sheep (n=10), Maltese goats (n=10), Ragusana donkeys (n=10), and Thoroughbred horses (n=10) were analyzed after storage at 23°C (room temperature) for 15 minutes (group I), 23°C for 1 hour (group II), 37°C for 8 hours (group III), and 4°C for 24 hours (group IV). Results were analyzed using a 1‐way repeated measures ANOVA. Results: In all species no statistically significant differences in pH values were present in samples stored at 4°C for 24 hours. This also was true for Pco2 in all species except the horse. Except for HCO3− concentration in the horse, significant changes in Po2, HCO3− concentration, base excess, and the standard bicarbonate concentration were observed for all species in samples stored at 4°C. In samples stored for only 1 hour at room temperature, significant changes in most analytes were detected. Conclusions: The results of this study underline the need for rapid assessment of acid‐base samples, because any delay, even for 1 hour, may affect the results.
ISSN:0275-6382
1939-165X
DOI:10.1111/j.1939-165X.2007.tb00441.x