Development of a heat shock inducible and inheritable RNAi system in silkworm
A heat shock inducible and inheritable RNA interference (RNAi) system was developed in the silkworm ( Bombyx mori). RNAi transgenic silkworms were generated by injecting silkworm eggs with a piggyBac transposon plasmid carrying RNAi sequence against target gene driven by the Drosophila heat shock pr...
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| Veröffentlicht in: | Biomolecular engineering 2007-12, Vol.24 (6), p.625-630 |
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| creator | Dai, Hongjiu Jiang, Rongjing Wang, Jue Xu, Guojiang Cao, Meixun Wang, Zhugang Fei, Jian |
| description | A heat shock inducible and inheritable RNA interference (RNAi) system was developed in the silkworm (
Bombyx mori). RNAi transgenic silkworms were generated by injecting silkworm eggs with a piggyBac transposon plasmid carrying RNAi sequence against target gene driven by the
Drosophila heat shock protein 70 (HSP70) promoter and the helper plasmid expressing piggyBac transposase. The transgenic EGFP gene and the endogenous eclosion hormone (EH) gene were chosen respectively as the target genes. In the RNAi transgenic silkworms, heat shock at 42
°C significantly and specifically reduced the expression of EGFP or EH gene in silkworms according to the corresponding RNAi targeting sequence but not in silkworms with the irrelevant RNAi sequence demonstrating the efficiency and specificity of the RNAi effect. Heat shock in the pupal stage hampered pupal–adult eclosion and reduced egg fertility in EH RNAi transgenic silkworms but not in the wild type or EGFP RNAi transgenic silkworms. The establishment of this heat inducible and inheritable conditional RNA interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages. |
| doi_str_mv | 10.1016/j.bioeng.2007.10.004 |
| format | Article |
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Bombyx mori). RNAi transgenic silkworms were generated by injecting silkworm eggs with a piggyBac transposon plasmid carrying RNAi sequence against target gene driven by the
Drosophila heat shock protein 70 (HSP70) promoter and the helper plasmid expressing piggyBac transposase. The transgenic EGFP gene and the endogenous eclosion hormone (EH) gene were chosen respectively as the target genes. In the RNAi transgenic silkworms, heat shock at 42
°C significantly and specifically reduced the expression of EGFP or EH gene in silkworms according to the corresponding RNAi targeting sequence but not in silkworms with the irrelevant RNAi sequence demonstrating the efficiency and specificity of the RNAi effect. Heat shock in the pupal stage hampered pupal–adult eclosion and reduced egg fertility in EH RNAi transgenic silkworms but not in the wild type or EGFP RNAi transgenic silkworms. The establishment of this heat inducible and inheritable conditional RNA interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages.</description><identifier>ISSN: 1389-0344</identifier><identifier>EISSN: 1878-559X</identifier><identifier>DOI: 10.1016/j.bioeng.2007.10.004</identifier><identifier>PMID: 18023613</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - physiology ; Bombyx - genetics ; Bombyx - metabolism ; Bombyx mori ; Cloning, Molecular ; Eclosion hormone ; Heat shock ; Heat-Shock Response - physiology ; HSP70 Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - metabolism ; Insect Proteins - genetics ; Insect Proteins - metabolism ; RNA Interference - physiology ; RNAi ; Transgenic silkworm ; Transposases - genetics ; Transposases - metabolism</subject><ispartof>Biomolecular engineering, 2007-12, Vol.24 (6), p.625-630</ispartof><rights>2007 Elsevier B.V.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-179a1adcb71be5491184fa22f8bfdd6f89ed0610e2fac9fcf5f9960b247098623</citedby><cites>FETCH-LOGICAL-c360t-179a1adcb71be5491184fa22f8bfdd6f89ed0610e2fac9fcf5f9960b247098623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18023613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Hongjiu</creatorcontrib><creatorcontrib>Jiang, Rongjing</creatorcontrib><creatorcontrib>Wang, Jue</creatorcontrib><creatorcontrib>Xu, Guojiang</creatorcontrib><creatorcontrib>Cao, Meixun</creatorcontrib><creatorcontrib>Wang, Zhugang</creatorcontrib><creatorcontrib>Fei, Jian</creatorcontrib><title>Development of a heat shock inducible and inheritable RNAi system in silkworm</title><title>Biomolecular engineering</title><addtitle>Biomol Eng</addtitle><description>A heat shock inducible and inheritable RNA interference (RNAi) system was developed in the silkworm (
Bombyx mori). RNAi transgenic silkworms were generated by injecting silkworm eggs with a piggyBac transposon plasmid carrying RNAi sequence against target gene driven by the
Drosophila heat shock protein 70 (HSP70) promoter and the helper plasmid expressing piggyBac transposase. The transgenic EGFP gene and the endogenous eclosion hormone (EH) gene were chosen respectively as the target genes. In the RNAi transgenic silkworms, heat shock at 42
°C significantly and specifically reduced the expression of EGFP or EH gene in silkworms according to the corresponding RNAi targeting sequence but not in silkworms with the irrelevant RNAi sequence demonstrating the efficiency and specificity of the RNAi effect. Heat shock in the pupal stage hampered pupal–adult eclosion and reduced egg fertility in EH RNAi transgenic silkworms but not in the wild type or EGFP RNAi transgenic silkworms. The establishment of this heat inducible and inheritable conditional RNA interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages.</description><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - physiology</subject><subject>Bombyx - genetics</subject><subject>Bombyx - metabolism</subject><subject>Bombyx mori</subject><subject>Cloning, Molecular</subject><subject>Eclosion hormone</subject><subject>Heat shock</subject><subject>Heat-Shock Response - physiology</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>RNA Interference - physiology</subject><subject>RNAi</subject><subject>Transgenic silkworm</subject><subject>Transposases - genetics</subject><subject>Transposases - metabolism</subject><issn>1389-0344</issn><issn>1878-559X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFtLxDAQhYMo3v-BSJ986zpp0zZ5EcQ7eAFR8C2kycTN2jZr0lX892bZBd98mpnDOTPMR8gRhQkFWp_OJq3zOLxPCoAmSRMAtkF2KW94XlXibTP1JRc5lIztkL0YZwCUASu3yQ7lUJQ1LXfJwyV-YefnPQ5j5m2msimqMYtTrz8yN5iFdm2HmRpMmqYY3KiW8_PjucviTxyxT3oWXffx7UN_QLas6iIerus-eb2-erm4ze-fbu4uzu9zXdYw5rQRiiqj24a2WDFBKWdWFYXlrTWmtlyggZoCFlZpYbWtrBA1tAVrQPC6KPfJyWrvPPjPBcZR9i5q7Do1oF9EWfOKNZRCMrKVUQcfY0Ar58H1KvxICnKJUc7kCqNcYlyqCWOKHa_3L9oezV9ozS0ZzlYGTF9-OQwyaoeDRuMC6lEa7_6_8AttF4WZ</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Dai, Hongjiu</creator><creator>Jiang, Rongjing</creator><creator>Wang, Jue</creator><creator>Xu, Guojiang</creator><creator>Cao, Meixun</creator><creator>Wang, Zhugang</creator><creator>Fei, Jian</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Development of a heat shock inducible and inheritable RNAi system in silkworm</title><author>Dai, Hongjiu ; Jiang, Rongjing ; Wang, Jue ; Xu, Guojiang ; Cao, Meixun ; Wang, Zhugang ; Fei, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-179a1adcb71be5491184fa22f8bfdd6f89ed0610e2fac9fcf5f9960b247098623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified - genetics</topic><topic>Animals, Genetically Modified - physiology</topic><topic>Bombyx - genetics</topic><topic>Bombyx - metabolism</topic><topic>Bombyx mori</topic><topic>Cloning, Molecular</topic><topic>Eclosion hormone</topic><topic>Heat shock</topic><topic>Heat-Shock Response - physiology</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>RNA Interference - physiology</topic><topic>RNAi</topic><topic>Transgenic silkworm</topic><topic>Transposases - genetics</topic><topic>Transposases - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Dai, Hongjiu</creatorcontrib><creatorcontrib>Jiang, Rongjing</creatorcontrib><creatorcontrib>Wang, Jue</creatorcontrib><creatorcontrib>Xu, Guojiang</creatorcontrib><creatorcontrib>Cao, Meixun</creatorcontrib><creatorcontrib>Wang, Zhugang</creatorcontrib><creatorcontrib>Fei, Jian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomolecular engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Hongjiu</au><au>Jiang, Rongjing</au><au>Wang, Jue</au><au>Xu, Guojiang</au><au>Cao, Meixun</au><au>Wang, Zhugang</au><au>Fei, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a heat shock inducible and inheritable RNAi system in silkworm</atitle><jtitle>Biomolecular engineering</jtitle><addtitle>Biomol Eng</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>24</volume><issue>6</issue><spage>625</spage><epage>630</epage><pages>625-630</pages><issn>1389-0344</issn><eissn>1878-559X</eissn><abstract>A heat shock inducible and inheritable RNA interference (RNAi) system was developed in the silkworm (
Bombyx mori). RNAi transgenic silkworms were generated by injecting silkworm eggs with a piggyBac transposon plasmid carrying RNAi sequence against target gene driven by the
Drosophila heat shock protein 70 (HSP70) promoter and the helper plasmid expressing piggyBac transposase. The transgenic EGFP gene and the endogenous eclosion hormone (EH) gene were chosen respectively as the target genes. In the RNAi transgenic silkworms, heat shock at 42
°C significantly and specifically reduced the expression of EGFP or EH gene in silkworms according to the corresponding RNAi targeting sequence but not in silkworms with the irrelevant RNAi sequence demonstrating the efficiency and specificity of the RNAi effect. Heat shock in the pupal stage hampered pupal–adult eclosion and reduced egg fertility in EH RNAi transgenic silkworms but not in the wild type or EGFP RNAi transgenic silkworms. The establishment of this heat inducible and inheritable conditional RNA interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18023613</pmid><doi>10.1016/j.bioeng.2007.10.004</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biomolecular engineering, 2007-12, Vol.24 (6), p.625-630 |
| issn | 1389-0344 1878-559X |
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| subjects | Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - physiology Bombyx - genetics Bombyx - metabolism Bombyx mori Cloning, Molecular Eclosion hormone Heat shock Heat-Shock Response - physiology HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - metabolism Insect Proteins - genetics Insect Proteins - metabolism RNA Interference - physiology RNAi Transgenic silkworm Transposases - genetics Transposases - metabolism |
| title | Development of a heat shock inducible and inheritable RNAi system in silkworm |
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