Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models
Abstract Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to tho...
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| Veröffentlicht in: | Vaccine 2007-11, Vol.25 (49), p.8203-8205 |
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| container_issue | 49 |
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| container_title | Vaccine |
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| creator | O'Sullivan, D.M Sander, C Shorten, R.J Gillespie, S.H Hill, A.V.S McHugh, T.D McShane, H Tchilian, E.Z |
| description | Abstract Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. |
| doi_str_mv | 10.1016/j.vaccine.2007.09.065 |
| format | Article |
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We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. 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We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples.</description><subject>Agar</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>CFU/ml</subject><subject>Colony Count, Microbial</subject><subject>Culture Media</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Humans</subject><subject>Lung - microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mouse</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Spleen - microbiology</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1r3DAQhkVpabZpf0KLT73ZGVn-kC4tJSRNIKGHpJBDQcjSGLS1raw-FvbfV2aXFnrJaRA8887oYQj5SKGiQLuLbbVXWtsFqxqgr0BU0LWvyIbynpV1S_lrsoG6a8qGwtMZeRfCFgBaRsVbckZ7wUGwfkN-Xe3VlFS0bincWEx2l6wpdJpi8liMzhe7pJZo41_k_qDdoHREb9NcxDSgz7gLNhR2Kebk807F7AxO4T15M6op4IdTPSc_r68eL2_Kux_fby-_3ZW6aUQsRzMyPgBnIx-waWgn8qtHA2oUHGk9GioMZ6JpOjFw09RZAG3btu5Yh1QJdk4-H3OfvdslDFHONmicJrWgS0F2PP-77_sXQSo6xmm7JrZHUHsXgsdRPns7K3-QFOTqX27lyb9c_UsQMvvPfZ9OA9Iwo_nXdRKega9HIOvBvUUvg7a4aDTWo47SOPviiC__JejJLlar6TceMGxd8kuWLakMtQT5sB7BegPQA4UWntgfzPSvUA</recordid><startdate>20071128</startdate><enddate>20071128</enddate><creator>O'Sullivan, D.M</creator><creator>Sander, C</creator><creator>Shorten, R.J</creator><creator>Gillespie, S.H</creator><creator>Hill, A.V.S</creator><creator>McHugh, T.D</creator><creator>McShane, H</creator><creator>Tchilian, E.Z</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20071128</creationdate><title>Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models</title><author>O'Sullivan, D.M ; Sander, C ; Shorten, R.J ; Gillespie, S.H ; Hill, A.V.S ; McHugh, T.D ; McShane, H ; Tchilian, E.Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-fdf38b083f8be441698b07ed0af98e12fd19d8394469b8d4210115552636e1a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agar</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Bacteriological Techniques</topic><topic>CFU/ml</topic><topic>Colony Count, Microbial</topic><topic>Culture Media</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Humans</topic><topic>Lung - microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mouse</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Spleen - microbiology</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Sullivan, D.M</creatorcontrib><creatorcontrib>Sander, C</creatorcontrib><creatorcontrib>Shorten, R.J</creatorcontrib><creatorcontrib>Gillespie, S.H</creatorcontrib><creatorcontrib>Hill, A.V.S</creatorcontrib><creatorcontrib>McHugh, T.D</creatorcontrib><creatorcontrib>McShane, H</creatorcontrib><creatorcontrib>Tchilian, E.Z</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Sullivan, D.M</au><au>Sander, C</au><au>Shorten, R.J</au><au>Gillespie, S.H</au><au>Hill, A.V.S</au><au>McHugh, T.D</au><au>McShane, H</au><au>Tchilian, E.Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2007-11-28</date><risdate>2007</risdate><volume>25</volume><issue>49</issue><spage>8203</spage><epage>8205</epage><pages>8203-8205</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Abstract Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. 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| ispartof | Vaccine, 2007-11, Vol.25 (49), p.8203-8205 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; ProQuest Central UK/Ireland |
| subjects | Agar Allergy and Immunology Animals Bacteriological Techniques CFU/ml Colony Count, Microbial Culture Media Disease Models, Animal Female Humans Lung - microbiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mouse Mycobacterium tuberculosis Mycobacterium tuberculosis - isolation & purification Sensitivity and Specificity Spleen - microbiology Tuberculosis, Pulmonary - microbiology |
| title | Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models |
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