Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies
Various types of embryoid body (EB) that were formed from mouse embryonic stem (ES) cells under various culture conditions were characterized in terms of gene expression pattern to estimate the differentiation status of the bodies. The gene expression of typical markers ( i.e., GATA-4, GATA-6, trans...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2007-10, Vol.104 (4), p.294-299 |
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| creator | Koike, Mikiko Sakaki, Shujiro Amano, Yoshifumi Kurosawa, Hiroshi |
| description | Various types of embryoid body (EB) that were formed from mouse embryonic stem (ES) cells under various culture conditions were characterized in terms of gene expression pattern to estimate the differentiation status of the bodies. The gene expression of typical markers (
i.e., GATA-4, GATA-6, transthyretin [TTR], α-fetoprotein [AFP], Nkx2.5, and α-myosin heavy chain [α-MHC]) was quantitatively analyzed in various types of EB, and the gene expression pattern of those marker genes was graphically shown for each EB. The gene expression pattern accurately represented the differentiation status of the EBs. The gene expression pattern indicated that the Nkx2.5 and α-MHC genes were highly expressed in the EBs formed from 1000 ES cells in a low-adherence 96-well plate. By transferring the EBs into an attachment culture, cardiomyocytes were more efficiently generated in the outgrowth of the EBs. When we increased the seeding cell number from 1000 to 4000 ES cells, the gene expression pattern changed, that is, the expression levels of the TTR and AFP genes increased, whereas those of the Nkx2.5 and α-MHC genes decreased, and the trend of differentiation changed from cardiomyogenesis to visceral yolk-sac-like structure formation. |
| doi_str_mv | 10.1263/jbb.104.294 |
| format | Article |
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i.e., GATA-4, GATA-6, transthyretin [TTR], α-fetoprotein [AFP], Nkx2.5, and α-myosin heavy chain [α-MHC]) was quantitatively analyzed in various types of EB, and the gene expression pattern of those marker genes was graphically shown for each EB. The gene expression pattern accurately represented the differentiation status of the EBs. The gene expression pattern indicated that the Nkx2.5 and α-MHC genes were highly expressed in the EBs formed from 1000 ES cells in a low-adherence 96-well plate. By transferring the EBs into an attachment culture, cardiomyocytes were more efficiently generated in the outgrowth of the EBs. When we increased the seeding cell number from 1000 to 4000 ES cells, the gene expression pattern changed, that is, the expression levels of the TTR and AFP genes increased, whereas those of the Nkx2.5 and α-MHC genes decreased, and the trend of differentiation changed from cardiomyogenesis to visceral yolk-sac-like structure formation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CELL CULTURE</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>CULTURE TECHNIQUES</subject><subject>differentiation</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - physiology</subject><subject>embryoid body</subject><subject>Embryonic Development - physiology</subject><subject>embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>MICE</subject><subject>MORFOLOGIA</subject><subject>MORPHOLOGIE</subject><subject>MORPHOLOGY</subject><subject>PCR</subject><subject>RATON</subject><subject>SOURIS</subject><subject>TECHNIQUE DE CULTURE</subject><subject>TECNICAS DE CULTIVO</subject><subject>Tissue Engineering - methods</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuKFTEQhhtRnHF05VoJiG7kHHPrS5bDwSsDutB1yKXi5NCdjEl6YHwb39T0dOOACC5CQtVHVf31p2meErwntGNvjlrvCeZ7Kvi95pQw3u84p-T-8h7EjvSUnTSPcj5iTHrck4fNCRkwZfWcNr8OlyopUyD5n6r4GFB0CCadbqK3SEfrIS-hKc4ZtkTwBuUCEzIwjhm5mCawaA4WErpWyVcUmXkscwJkYrB-qZuRChZBLn7608d65yBBKH4N5aLKfNsuz-Zy6_64eeDUmOHJdp813969_Xr4sLv4_P7j4fxiZ3g3lB03oAQ3HTGEEq00hs6B1kw71bPBUuZMa6hzQjigjhNiWCusJq5zQ-c4ZmfNq7XuVYo_5jqonHxeFKoAVZHshpa2ApP_gkS0tBeCVfDFX-AxzilUEZJwThitkKjU65UyKeacwMmrVHeUbiTBcjFYVoPrm8tqcKWfbzVnXbd-x26OVuDlBqhs1OiSCsbnO05QOnS3cp-tnFNRqu-pMp--UIyH-kta3NZ8u-ah7vzaQ5LZeAgGrE9girTR_3PA374xzUs</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Koike, Mikiko</creator><creator>Sakaki, Shujiro</creator><creator>Amano, Yoshifumi</creator><creator>Kurosawa, Hiroshi</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies</title><author>Koike, Mikiko ; Sakaki, Shujiro ; Amano, Yoshifumi ; Kurosawa, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-4cea94c61c121bab0e6febb3bfa738d23fc5c2ff99fe2f411c359db1f6f86f403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CELL CULTURE</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>CULTURE TECHNIQUES</topic><topic>differentiation</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - physiology</topic><topic>embryoid body</topic><topic>Embryonic Development - physiology</topic><topic>embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>MICE</topic><topic>MORFOLOGIA</topic><topic>MORPHOLOGIE</topic><topic>MORPHOLOGY</topic><topic>PCR</topic><topic>RATON</topic><topic>SOURIS</topic><topic>TECHNIQUE DE CULTURE</topic><topic>TECNICAS DE CULTIVO</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koike, Mikiko</creatorcontrib><creatorcontrib>Sakaki, Shujiro</creatorcontrib><creatorcontrib>Amano, Yoshifumi</creatorcontrib><creatorcontrib>Kurosawa, Hiroshi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koike, Mikiko</au><au>Sakaki, Shujiro</au><au>Amano, Yoshifumi</au><au>Kurosawa, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>104</volume><issue>4</issue><spage>294</spage><epage>299</epage><pages>294-299</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><coden>JFBIEX</coden><abstract>Various types of embryoid body (EB) that were formed from mouse embryonic stem (ES) cells under various culture conditions were characterized in terms of gene expression pattern to estimate the differentiation status of the bodies. The gene expression of typical markers (
i.e., GATA-4, GATA-6, transthyretin [TTR], α-fetoprotein [AFP], Nkx2.5, and α-myosin heavy chain [α-MHC]) was quantitatively analyzed in various types of EB, and the gene expression pattern of those marker genes was graphically shown for each EB. The gene expression pattern accurately represented the differentiation status of the EBs. The gene expression pattern indicated that the Nkx2.5 and α-MHC genes were highly expressed in the EBs formed from 1000 ES cells in a low-adherence 96-well plate. By transferring the EBs into an attachment culture, cardiomyocytes were more efficiently generated in the outgrowth of the EBs. When we increased the seeding cell number from 1000 to 4000 ES cells, the gene expression pattern changed, that is, the expression levels of the TTR and AFP genes increased, whereas those of the Nkx2.5 and α-MHC genes decreased, and the trend of differentiation changed from cardiomyogenesis to visceral yolk-sac-like structure formation.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>18023802</pmid><doi>10.1263/jbb.104.294</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biological and medical sciences Biotechnology CELL CULTURE Cell Culture Techniques - methods Cell Differentiation Cell Line CULTIVO DE CELULAS CULTURE DE CELLULE CULTURE TECHNIQUES differentiation Embryo, Mammalian - cytology Embryo, Mammalian - physiology embryoid body Embryonic Development - physiology embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Fundamental and applied biological sciences. Psychology MICE MORFOLOGIA MORPHOLOGIE MORPHOLOGY PCR RATON SOURIS TECHNIQUE DE CULTURE TECNICAS DE CULTIVO Tissue Engineering - methods |
| title | Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies |
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