A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells
We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma n...
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| Veröffentlicht in: | FEBS letters 2007-11, Vol.581 (28), p.5382-5388 |
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| creator | Zeerleder, Sacha Zwart, Bas te Velthuis, Henk Manoe, Rishi Bulder, Ingrid Rensink, Irma Aarden, Lucien A. |
| description | We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. |
| doi_str_mv | 10.1016/j.febslet.2007.10.037 |
| format | Article |
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The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2007.10.037</identifier><identifier>PMID: 17976375</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Apoptosis ; arbitrary unit ; CM ; culture medium ; culture medium without FCS ; Enzyme Activation ; Fibrinolysin - metabolism ; Humans ; Jurkat Cells ; median fluorescence intensity ; MFI ; Necrosis - enzymology ; Necrosis - pathology ; NRF ; Nucleosome ; Nucleosome releasing factor ; Nucleosomes - drug effects ; Nucleosomes - enzymology ; phenylmethylsulfonylfluoride ; Plasminogen - metabolism ; PMSF ; Propidium ; propidium iodide ; Protease ; Protease Inhibitors - pharmacology ; SBTI ; secondary necrotic ; Serine Endopeptidases - metabolism ; soybean trypsin inhibitor ; tissue-type plasminogen activator ; tPA ; uPA ; urokinase-type plasminogen activator</subject><ispartof>FEBS letters, 2007-11, Vol.581 (28), p.5382-5388</ispartof><rights>2007 Federation of European Biochemical Societies</rights><rights>FEBS Letters 581 (2007) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4597-cb9c9b5ff678e108b9367394be50fac1388d933ab8f7d88ee4881b645f58de9f3</citedby><cites>FETCH-LOGICAL-c4597-cb9c9b5ff678e108b9367394be50fac1388d933ab8f7d88ee4881b645f58de9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2007.10.037$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579307011027$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3536,27903,27904,45553,45554,46387,46811,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17976375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeerleder, Sacha</creatorcontrib><creatorcontrib>Zwart, Bas</creatorcontrib><creatorcontrib>te Velthuis, Henk</creatorcontrib><creatorcontrib>Manoe, Rishi</creatorcontrib><creatorcontrib>Bulder, Ingrid</creatorcontrib><creatorcontrib>Rensink, Irma</creatorcontrib><creatorcontrib>Aarden, Lucien A.</creatorcontrib><title>A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells.</description><subject>Apoptosis</subject><subject>arbitrary unit</subject><subject>CM</subject><subject>culture medium</subject><subject>culture medium without FCS</subject><subject>Enzyme Activation</subject><subject>Fibrinolysin - metabolism</subject><subject>Humans</subject><subject>Jurkat Cells</subject><subject>median fluorescence intensity</subject><subject>MFI</subject><subject>Necrosis - enzymology</subject><subject>Necrosis - pathology</subject><subject>NRF</subject><subject>Nucleosome</subject><subject>Nucleosome releasing factor</subject><subject>Nucleosomes - drug effects</subject><subject>Nucleosomes - enzymology</subject><subject>phenylmethylsulfonylfluoride</subject><subject>Plasminogen - metabolism</subject><subject>PMSF</subject><subject>Propidium</subject><subject>propidium iodide</subject><subject>Protease</subject><subject>Protease Inhibitors - pharmacology</subject><subject>SBTI</subject><subject>secondary necrotic</subject><subject>Serine Endopeptidases - metabolism</subject><subject>soybean trypsin inhibitor</subject><subject>tissue-type plasminogen activator</subject><subject>tPA</subject><subject>uPA</subject><subject>urokinase-type plasminogen activator</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNklFvFCEQx4nR2LP6ETQ8mfqwJxzLAk-mbXqtSaOJrc9klx2UCwsn7F5z36IfuWzuEn2rTzCT__xmhj8IvadkSQltPm-WFrrsYVyuCBEltyRMvEALKgWrWN3Il2hBCK0rLhQ7QW9y3pASS6peoxMqlGiY4Av0eI63vs1Di8NkPMQcB8AJPLTZhV_YtmaMCZ99-7H-hB_c-BtnSC4A3qY4Fg3gInA7N-6xy9iFPKZpgDC2vgSFM8RduUb7Dz1jm-JQOCaGvk17HMAUmDPYgPf5LXplW5_h3fE8RT_XV_eXN9Xt9-uvl-e3lam5EpXplFEdt7YREiiRnWKNYKrugJMyNGVS9oqxtpNW9FIC1FLSrqm55bIHZdkp-njglk3-TJBHPbg8T9AGiFPWjeQrLln9rJAqQQRrZiE_CMs6OSewepvcUDbUlOjZM73RR8_07NmcLp6Vug_HBlM3QP-36mhSEdwcBA_Ow_7_qHp9dbG6mz_A7D8RhFKymnt9OaCgPO3OQdLZOAgGepfAjLqP7plpnwAXO8Nh</recordid><startdate>20071127</startdate><enddate>20071127</enddate><creator>Zeerleder, Sacha</creator><creator>Zwart, Bas</creator><creator>te Velthuis, Henk</creator><creator>Manoe, Rishi</creator><creator>Bulder, Ingrid</creator><creator>Rensink, Irma</creator><creator>Aarden, Lucien A.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20071127</creationdate><title>A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells</title><author>Zeerleder, Sacha ; Zwart, Bas ; te Velthuis, Henk ; Manoe, Rishi ; Bulder, Ingrid ; Rensink, Irma ; Aarden, Lucien A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4597-cb9c9b5ff678e108b9367394be50fac1388d933ab8f7d88ee4881b645f58de9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Apoptosis</topic><topic>arbitrary unit</topic><topic>CM</topic><topic>culture medium</topic><topic>culture medium without FCS</topic><topic>Enzyme Activation</topic><topic>Fibrinolysin - metabolism</topic><topic>Humans</topic><topic>Jurkat Cells</topic><topic>median fluorescence intensity</topic><topic>MFI</topic><topic>Necrosis - enzymology</topic><topic>Necrosis - pathology</topic><topic>NRF</topic><topic>Nucleosome</topic><topic>Nucleosome releasing factor</topic><topic>Nucleosomes - drug effects</topic><topic>Nucleosomes - enzymology</topic><topic>phenylmethylsulfonylfluoride</topic><topic>Plasminogen - metabolism</topic><topic>PMSF</topic><topic>Propidium</topic><topic>propidium iodide</topic><topic>Protease</topic><topic>Protease Inhibitors - pharmacology</topic><topic>SBTI</topic><topic>secondary necrotic</topic><topic>Serine Endopeptidases - metabolism</topic><topic>soybean trypsin inhibitor</topic><topic>tissue-type plasminogen activator</topic><topic>tPA</topic><topic>uPA</topic><topic>urokinase-type plasminogen activator</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeerleder, Sacha</creatorcontrib><creatorcontrib>Zwart, Bas</creatorcontrib><creatorcontrib>te Velthuis, Henk</creatorcontrib><creatorcontrib>Manoe, Rishi</creatorcontrib><creatorcontrib>Bulder, Ingrid</creatorcontrib><creatorcontrib>Rensink, Irma</creatorcontrib><creatorcontrib>Aarden, Lucien A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeerleder, Sacha</au><au>Zwart, Bas</au><au>te Velthuis, Henk</au><au>Manoe, Rishi</au><au>Bulder, Ingrid</au><au>Rensink, Irma</au><au>Aarden, Lucien A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2007-11-27</date><risdate>2007</risdate><volume>581</volume><issue>28</issue><spage>5382</spage><epage>5388</epage><pages>5382-5388</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>17976375</pmid><doi>10.1016/j.febslet.2007.10.037</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Apoptosis arbitrary unit CM culture medium culture medium without FCS Enzyme Activation Fibrinolysin - metabolism Humans Jurkat Cells median fluorescence intensity MFI Necrosis - enzymology Necrosis - pathology NRF Nucleosome Nucleosome releasing factor Nucleosomes - drug effects Nucleosomes - enzymology phenylmethylsulfonylfluoride Plasminogen - metabolism PMSF Propidium propidium iodide Protease Protease Inhibitors - pharmacology SBTI secondary necrotic Serine Endopeptidases - metabolism soybean trypsin inhibitor tissue-type plasminogen activator tPA uPA urokinase-type plasminogen activator |
| title | A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells |
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