Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages
Natural products are known to be sources of bioactive components exerting antioxidative and anti-inflammatory activities. We evaluated the suppressive effects of the methanol extract (0-45 microg/mL) of the aerial parts of Saururus chinensis (Lour.) Baill (Saururaceae) on lipopolysaccharide (LPS)-st...
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| Veröffentlicht in: | Journal of medicinal food 2005-06, Vol.8 (2), p.190-197 |
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| creator | Cho, H.Y Cho, C.W Song, Y.S |
| description | Natural products are known to be sources of bioactive components exerting antioxidative and anti-inflammatory activities. We evaluated the suppressive effects of the methanol extract (0-45 microg/mL) of the aerial parts of Saururus chinensis (Lour.) Baill (Saururaceae) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and oxidative stress buildup in the RAW 264.7 murine macrophages. Treatment of RAW 264.7 cells with S. chinensis methanol extract (SME) significantly reduced LPS-stimulated NO production in a concentration-dependent manner. Treatment with SME reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione levels and activities of antioxidative enzymes, including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in LPS-stimulated macrophages compared with LPS-only treated cells. Expression of inducible NO synthase (iNOS) mRNA was also suppressed in SMEtreated cells. The specific DNA binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from SME-treated cells were significantly suppressed. These results suggest that SME has antioxidative and anti-inflammatory activities by enhancing antioxidative defense systems and suppressing NO production via the down-regulation of iNOS expression and NFkappaB activity. |
| doi_str_mv | 10.1089/jmf.2005.8.190 |
| format | Article |
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We evaluated the suppressive effects of the methanol extract (0-45 microg/mL) of the aerial parts of Saururus chinensis (Lour.) Baill (Saururaceae) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and oxidative stress buildup in the RAW 264.7 murine macrophages. Treatment of RAW 264.7 cells with S. chinensis methanol extract (SME) significantly reduced LPS-stimulated NO production in a concentration-dependent manner. Treatment with SME reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione levels and activities of antioxidative enzymes, including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in LPS-stimulated macrophages compared with LPS-only treated cells. Expression of inducible NO synthase (iNOS) mRNA was also suppressed in SMEtreated cells. The specific DNA binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from SME-treated cells were significantly suppressed. These results suggest that SME has antioxidative and anti-inflammatory activities by enhancing antioxidative defense systems and suppressing NO production via the down-regulation of iNOS expression and NFkappaB activity.</description><identifier>ISSN: 1096-620X</identifier><identifier>EISSN: 1557-7600</identifier><identifier>DOI: 10.1089/jmf.2005.8.190</identifier><identifier>PMID: 16117611</identifier><language>eng</language><publisher>United States</publisher><subject>aerial parts ; Animals ; anti-inflammatory activity ; Anti-Inflammatory Agents - pharmacology ; antioxidant activity ; antioxidants ; Antioxidants - pharmacology ; Cells, Cultured ; cytokines ; Dose-Response Relationship, Drug ; extraction ; free radicals ; invasive species ; lipid peroxidation ; Lipid Peroxidation - drug effects ; Lipopolysaccharides ; Liver - drug effects ; Liver - enzymology ; macrophages ; Macrophages - drug effects ; methanol ; Mice ; NF-kappa B - metabolism ; nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - metabolism ; Nitric Oxide Synthase Type II ; oxidative stress ; Oxidative Stress - drug effects ; plant extracts ; Plant Extracts - pharmacology ; RNA, Messenger - metabolism ; Saururaceae - chemistry ; Saururus ; Thiobarbituric Acid Reactive Substances - analysis ; Thiobarbituric Acid Reactive Substances - metabolism ; thiobarbituric acid-reactive substances ; weeds</subject><ispartof>Journal of medicinal food, 2005-06, Vol.8 (2), p.190-197</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-6cab49a7efc8da47b32ae6452296a4866f0bd041acf66e9762b67ec8c618fd223</citedby><cites>FETCH-LOGICAL-c383t-6cab49a7efc8da47b32ae6452296a4866f0bd041acf66e9762b67ec8c618fd223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3043,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16117611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, H.Y</creatorcontrib><creatorcontrib>Cho, C.W</creatorcontrib><creatorcontrib>Song, Y.S</creatorcontrib><title>Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages</title><title>Journal of medicinal food</title><addtitle>J Med Food</addtitle><description>Natural products are known to be sources of bioactive components exerting antioxidative and anti-inflammatory activities. We evaluated the suppressive effects of the methanol extract (0-45 microg/mL) of the aerial parts of Saururus chinensis (Lour.) Baill (Saururaceae) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and oxidative stress buildup in the RAW 264.7 murine macrophages. Treatment of RAW 264.7 cells with S. chinensis methanol extract (SME) significantly reduced LPS-stimulated NO production in a concentration-dependent manner. Treatment with SME reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione levels and activities of antioxidative enzymes, including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in LPS-stimulated macrophages compared with LPS-only treated cells. Expression of inducible NO synthase (iNOS) mRNA was also suppressed in SMEtreated cells. The specific DNA binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from SME-treated cells were significantly suppressed. These results suggest that SME has antioxidative and anti-inflammatory activities by enhancing antioxidative defense systems and suppressing NO production via the down-regulation of iNOS expression and NFkappaB activity.</description><subject>aerial parts</subject><subject>Animals</subject><subject>anti-inflammatory activity</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Cells, Cultured</subject><subject>cytokines</subject><subject>Dose-Response Relationship, Drug</subject><subject>extraction</subject><subject>free radicals</subject><subject>invasive species</subject><subject>lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Lipopolysaccharides</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>macrophages</subject><subject>Macrophages - drug effects</subject><subject>methanol</subject><subject>Mice</subject><subject>NF-kappa B - metabolism</subject><subject>nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitric Oxide Synthase Type II</subject><subject>oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>RNA, Messenger - metabolism</subject><subject>Saururaceae - chemistry</subject><subject>Saururus</subject><subject>Thiobarbituric Acid Reactive Substances - analysis</subject><subject>Thiobarbituric Acid Reactive Substances - metabolism</subject><subject>thiobarbituric acid-reactive substances</subject><subject>weeds</subject><issn>1096-620X</issn><issn>1557-7600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1rHDEMhk1JaL567bH1qbeZyh6P7TkuIW0KC4EmIb0ZjcfOOp2Pje0Nyb-vwy4U8SIdHgnxEPKZQc1Ad9-fJl9zgLbWNevgAzllbasqJQGOygydrCSHPyfkLKUnAGhEoz6SEyYZUyWn5O9qzmF5DQPm8OIozkNJDlWY_YjThHmJb9R572xOdPH0FnexVKJ2E2Y3p5Do5PIG52Wk7jVHtJmGmf5ePVAuRa3ohDYu2w0-unRBjj2OyX069HNy_-Pq7vK6Wt_8_HW5Wle20U2upMVedKict3pAofqGo5Oi5byTKLSUHvoBBEPrpXSdkryXylltJdN-4Lw5J9_2d7dxed65lM0UknXjiLNbdslI3XLohChgvQfLiylF5802hgnjm2Fg3vWaote86zXaFL1l4cvh8q6f3PAfP_gswNc94HEx-BhDMve3HFgDDJhqlWr-Aax9gIc</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Cho, H.Y</creator><creator>Cho, C.W</creator><creator>Song, Y.S</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages</title><author>Cho, H.Y ; Cho, C.W ; Song, Y.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-6cab49a7efc8da47b32ae6452296a4866f0bd041acf66e9762b67ec8c618fd223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>aerial parts</topic><topic>Animals</topic><topic>anti-inflammatory activity</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Cells, Cultured</topic><topic>cytokines</topic><topic>Dose-Response Relationship, Drug</topic><topic>extraction</topic><topic>free radicals</topic><topic>invasive species</topic><topic>lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Lipopolysaccharides</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>macrophages</topic><topic>Macrophages - drug effects</topic><topic>methanol</topic><topic>Mice</topic><topic>NF-kappa B - metabolism</topic><topic>nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitric Oxide Synthase Type II</topic><topic>oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>plant extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>RNA, Messenger - metabolism</topic><topic>Saururaceae - chemistry</topic><topic>Saururus</topic><topic>Thiobarbituric Acid Reactive Substances - analysis</topic><topic>Thiobarbituric Acid Reactive Substances - metabolism</topic><topic>thiobarbituric acid-reactive substances</topic><topic>weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, H.Y</creatorcontrib><creatorcontrib>Cho, C.W</creatorcontrib><creatorcontrib>Song, Y.S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medicinal food</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, H.Y</au><au>Cho, C.W</au><au>Song, Y.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages</atitle><jtitle>Journal of medicinal food</jtitle><addtitle>J Med Food</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>8</volume><issue>2</issue><spage>190</spage><epage>197</epage><pages>190-197</pages><issn>1096-620X</issn><eissn>1557-7600</eissn><abstract>Natural products are known to be sources of bioactive components exerting antioxidative and anti-inflammatory activities. We evaluated the suppressive effects of the methanol extract (0-45 microg/mL) of the aerial parts of Saururus chinensis (Lour.) Baill (Saururaceae) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and oxidative stress buildup in the RAW 264.7 murine macrophages. Treatment of RAW 264.7 cells with S. chinensis methanol extract (SME) significantly reduced LPS-stimulated NO production in a concentration-dependent manner. Treatment with SME reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione levels and activities of antioxidative enzymes, including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in LPS-stimulated macrophages compared with LPS-only treated cells. Expression of inducible NO synthase (iNOS) mRNA was also suppressed in SMEtreated cells. The specific DNA binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from SME-treated cells were significantly suppressed. These results suggest that SME has antioxidative and anti-inflammatory activities by enhancing antioxidative defense systems and suppressing NO production via the down-regulation of iNOS expression and NFkappaB activity.</abstract><cop>United States</cop><pmid>16117611</pmid><doi>10.1089/jmf.2005.8.190</doi><tpages>8</tpages></addata></record> |
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| subjects | aerial parts Animals anti-inflammatory activity Anti-Inflammatory Agents - pharmacology antioxidant activity antioxidants Antioxidants - pharmacology Cells, Cultured cytokines Dose-Response Relationship, Drug extraction free radicals invasive species lipid peroxidation Lipid Peroxidation - drug effects Lipopolysaccharides Liver - drug effects Liver - enzymology macrophages Macrophages - drug effects methanol Mice NF-kappa B - metabolism nitric oxide Nitric Oxide - biosynthesis Nitric Oxide Synthase - metabolism Nitric Oxide Synthase Type II oxidative stress Oxidative Stress - drug effects plant extracts Plant Extracts - pharmacology RNA, Messenger - metabolism Saururaceae - chemistry Saururus Thiobarbituric Acid Reactive Substances - analysis Thiobarbituric Acid Reactive Substances - metabolism thiobarbituric acid-reactive substances weeds |
| title | Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages |
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