Cyclin D1 protein and CCND1 gene expression in colorectal cancer
To report the expression of cyclin D1 protein and its gene in a series of colorectal adenocarcinoma. One hundred and eleven specimens of colorectal carcinomas and adjacent normal colorectal mucosa were investigated by staining with a monoclonal antibody against cyclin D1 and by RT-PCR. Expression of...
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| Veröffentlicht in: | European journal of surgical oncology 2005-09, Vol.31 (7), p.721-726 |
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| container_title | European journal of surgical oncology |
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| creator | Balcerczak, E. Pasz-Walczak, G. Kumor, P. Panczyk, M. Kordek, R. Wierzbicki, R. Mirowski, M. |
| description | To report the expression of cyclin D1 protein and its gene in a series of colorectal adenocarcinoma.
One hundred and eleven specimens of colorectal carcinomas and adjacent normal colorectal mucosa were investigated by staining with a monoclonal antibody against cyclin D1 and by RT-PCR.
Expression of CCND1 gene was found in 54 out of 111 cases of colorectal cancers, while in normal mucosa the expression of this gene was not observed. Cyclin D1 protein expression was checked in the same group of adenocarcinoma cases. Presence of this protein was observed in 69 cases and for 43 of them also expression of its gene was found. Dependence between the presence of protein and the gene expression was statistically significant (p=0.0002). In the group of cases where CCND1 gene expression was detected, high level of its protein expression was found in 20 cases. The CCND1 gene expression was associated with metastases to lymph nodes (p=0.0181) and also with distant metastasis (p=0.0204).
The combined measurement of both the gene and its protein product, is an important contribution to the study of molecular markers in histological material. |
| doi_str_mv | 10.1016/j.ejso.2005.04.005 |
| format | Article |
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One hundred and eleven specimens of colorectal carcinomas and adjacent normal colorectal mucosa were investigated by staining with a monoclonal antibody against cyclin D1 and by RT-PCR.
Expression of CCND1 gene was found in 54 out of 111 cases of colorectal cancers, while in normal mucosa the expression of this gene was not observed. Cyclin D1 protein expression was checked in the same group of adenocarcinoma cases. Presence of this protein was observed in 69 cases and for 43 of them also expression of its gene was found. Dependence between the presence of protein and the gene expression was statistically significant (p=0.0002). In the group of cases where CCND1 gene expression was detected, high level of its protein expression was found in 20 cases. The CCND1 gene expression was associated with metastases to lymph nodes (p=0.0181) and also with distant metastasis (p=0.0204).
The combined measurement of both the gene and its protein product, is an important contribution to the study of molecular markers in histological material.</description><identifier>ISSN: 0748-7983</identifier><identifier>EISSN: 1532-2157</identifier><identifier>DOI: 10.1016/j.ejso.2005.04.005</identifier><identifier>PMID: 15993030</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Aged ; Antibodies, Monoclonal ; Biomarkers, Tumor - analysis ; Case-Control Studies ; CCND1 gene ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Cyclin D1 - biosynthesis ; Cyclin D1 - genetics ; Cyclin D1 protein ; Female ; Gene Expression Profiling ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Neoplasm Metastasis - genetics ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>European journal of surgical oncology, 2005-09, Vol.31 (7), p.721-726</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-3d1417fe18f12bdcfffed2a1e9cc0e5f1aae762366056a0d63c4a8eab350532e3</citedby><cites>FETCH-LOGICAL-c354t-3d1417fe18f12bdcfffed2a1e9cc0e5f1aae762366056a0d63c4a8eab350532e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejso.2005.04.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15993030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balcerczak, E.</creatorcontrib><creatorcontrib>Pasz-Walczak, G.</creatorcontrib><creatorcontrib>Kumor, P.</creatorcontrib><creatorcontrib>Panczyk, M.</creatorcontrib><creatorcontrib>Kordek, R.</creatorcontrib><creatorcontrib>Wierzbicki, R.</creatorcontrib><creatorcontrib>Mirowski, M.</creatorcontrib><title>Cyclin D1 protein and CCND1 gene expression in colorectal cancer</title><title>European journal of surgical oncology</title><addtitle>Eur J Surg Oncol</addtitle><description>To report the expression of cyclin D1 protein and its gene in a series of colorectal adenocarcinoma.
One hundred and eleven specimens of colorectal carcinomas and adjacent normal colorectal mucosa were investigated by staining with a monoclonal antibody against cyclin D1 and by RT-PCR.
Expression of CCND1 gene was found in 54 out of 111 cases of colorectal cancers, while in normal mucosa the expression of this gene was not observed. Cyclin D1 protein expression was checked in the same group of adenocarcinoma cases. Presence of this protein was observed in 69 cases and for 43 of them also expression of its gene was found. Dependence between the presence of protein and the gene expression was statistically significant (p=0.0002). In the group of cases where CCND1 gene expression was detected, high level of its protein expression was found in 20 cases. The CCND1 gene expression was associated with metastases to lymph nodes (p=0.0181) and also with distant metastasis (p=0.0204).
The combined measurement of both the gene and its protein product, is an important contribution to the study of molecular markers in histological material.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Aged</subject><subject>Antibodies, Monoclonal</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Case-Control Studies</subject><subject>CCND1 gene</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Cyclin D1 - biosynthesis</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 protein</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Metastasis - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>0748-7983</issn><issn>1532-2157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMouq5-AQ_Sk7fWSdKkLXhQ6l9Y9KLnkE0m0tJt16Qr7rc3ZRe8eXrDzJvHzI-QCwoZBSqv2wzbMGQMQGSQZ1EOyIwKzlJGRXFIZlDkZVpUJT8hpyG0AFDxojomJ1RUFQcOM3Jbb03X9Mk9TdZ-GDGWurdJXb_Gzif2mODP2mMIzdAncWiGbvBoRt0lRvcG_Rk5croLeL7XOfl4fHivn9PF29NLfbdIDRf5mHJLc1o4pKWjbGmNcw4t0xQrYwCFo1pjIRmXEoTUYCU3uS5RL7mA-BHyObna5cYzvzYYRrVqgsGu0z0Om6BkKRjksopGtjMaP4Tg0am1b1babxUFNXFTrZq4qYmbglxFiUuX-_TNcoX2b2UPKhpudgaMP3436FUwDUYAtplwKDs0_-X_AlgNffg</recordid><startdate>200509</startdate><enddate>200509</enddate><creator>Balcerczak, E.</creator><creator>Pasz-Walczak, G.</creator><creator>Kumor, P.</creator><creator>Panczyk, M.</creator><creator>Kordek, R.</creator><creator>Wierzbicki, R.</creator><creator>Mirowski, M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200509</creationdate><title>Cyclin D1 protein and CCND1 gene expression in colorectal cancer</title><author>Balcerczak, E. ; Pasz-Walczak, G. ; Kumor, P. ; Panczyk, M. ; Kordek, R. ; Wierzbicki, R. ; Mirowski, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-3d1417fe18f12bdcfffed2a1e9cc0e5f1aae762366056a0d63c4a8eab350532e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - pathology</topic><topic>Aged</topic><topic>Antibodies, Monoclonal</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Case-Control Studies</topic><topic>CCND1 gene</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Cyclin D1 - biosynthesis</topic><topic>Cyclin D1 - genetics</topic><topic>Cyclin D1 protein</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Metastasis - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balcerczak, E.</creatorcontrib><creatorcontrib>Pasz-Walczak, G.</creatorcontrib><creatorcontrib>Kumor, P.</creatorcontrib><creatorcontrib>Panczyk, M.</creatorcontrib><creatorcontrib>Kordek, R.</creatorcontrib><creatorcontrib>Wierzbicki, R.</creatorcontrib><creatorcontrib>Mirowski, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balcerczak, E.</au><au>Pasz-Walczak, G.</au><au>Kumor, P.</au><au>Panczyk, M.</au><au>Kordek, R.</au><au>Wierzbicki, R.</au><au>Mirowski, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclin D1 protein and CCND1 gene expression in colorectal cancer</atitle><jtitle>European journal of surgical oncology</jtitle><addtitle>Eur J Surg Oncol</addtitle><date>2005-09</date><risdate>2005</risdate><volume>31</volume><issue>7</issue><spage>721</spage><epage>726</epage><pages>721-726</pages><issn>0748-7983</issn><eissn>1532-2157</eissn><abstract>To report the expression of cyclin D1 protein and its gene in a series of colorectal adenocarcinoma.
One hundred and eleven specimens of colorectal carcinomas and adjacent normal colorectal mucosa were investigated by staining with a monoclonal antibody against cyclin D1 and by RT-PCR.
Expression of CCND1 gene was found in 54 out of 111 cases of colorectal cancers, while in normal mucosa the expression of this gene was not observed. Cyclin D1 protein expression was checked in the same group of adenocarcinoma cases. Presence of this protein was observed in 69 cases and for 43 of them also expression of its gene was found. Dependence between the presence of protein and the gene expression was statistically significant (p=0.0002). In the group of cases where CCND1 gene expression was detected, high level of its protein expression was found in 20 cases. The CCND1 gene expression was associated with metastases to lymph nodes (p=0.0181) and also with distant metastasis (p=0.0204).
The combined measurement of both the gene and its protein product, is an important contribution to the study of molecular markers in histological material.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15993030</pmid><doi>10.1016/j.ejso.2005.04.005</doi><tpages>6</tpages></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - pathology Aged Antibodies, Monoclonal Biomarkers, Tumor - analysis Case-Control Studies CCND1 gene Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Cyclin D1 - biosynthesis Cyclin D1 - genetics Cyclin D1 protein Female Gene Expression Profiling Humans Immunohistochemistry Male Middle Aged Neoplasm Metastasis - genetics Reverse Transcriptase Polymerase Chain Reaction |
| title | Cyclin D1 protein and CCND1 gene expression in colorectal cancer |
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