Autoantibodies to human alpha6 integrin in patients with bullous pemphigoid
Bullous pemphigoid (BP) is characterized immunologically by tissue-bound and circulating autoantibodies targeting the hemidesmosomal proteins BP230 and BP180. Recent evidence suggests a pathophysiological role for autoantibodies against alpha6 integrin in the subepidermal blister formation of oral p...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2005-06, Vol.1051, p.104-110 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | Kiss, Mária Perényi, Adám Marczinovits, Ilona Molnár, János Dobozy, Attila Kemény, Lajos Husz, Sándor |
| description | Bullous pemphigoid (BP) is characterized immunologically by tissue-bound and circulating autoantibodies targeting the hemidesmosomal proteins BP230 and BP180. Recent evidence suggests a pathophysiological role for autoantibodies against alpha6 integrin in the subepidermal blister formation of oral pemphigoid. The objective of our study was to investigate the presence of anti-alpha6 integrin antibodies in patients with classical BP. The autoantibody profiles of 30 patients with BP, 10 patients with pemphigus vulgaris, and 20 healthy persons were identified. With the use of PeptideStructure and PlotStructure software, four different antigenic epitopes for alpha6 integrin were predicted, and their fusion recombinant constructs were prepared in an E. coli expression system. Sera were tested for alpha6 integrin autoantibodies by an ELISA technique. Altogether, 52% of the patients with BP displayed circulating antibodies against at least one recombinant protein. Our findings provide the first evidence for the presence of anti-alpha6 integrin antibodies in patients with classical BP. |
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Recent evidence suggests a pathophysiological role for autoantibodies against alpha6 integrin in the subepidermal blister formation of oral pemphigoid. The objective of our study was to investigate the presence of anti-alpha6 integrin antibodies in patients with classical BP. The autoantibody profiles of 30 patients with BP, 10 patients with pemphigus vulgaris, and 20 healthy persons were identified. With the use of PeptideStructure and PlotStructure software, four different antigenic epitopes for alpha6 integrin were predicted, and their fusion recombinant constructs were prepared in an E. coli expression system. Sera were tested for alpha6 integrin autoantibodies by an ELISA technique. Altogether, 52% of the patients with BP displayed circulating antibodies against at least one recombinant protein. Our findings provide the first evidence for the presence of anti-alpha6 integrin antibodies in patients with classical BP.</description><identifier>ISSN: 0077-8923</identifier><identifier>PMID: 16126949</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Autoantibodies - blood ; Female ; Humans ; Integrin alpha6 - immunology ; Male ; Middle Aged ; Pemphigoid, Bullous - immunology</subject><ispartof>Annals of the New York Academy of Sciences, 2005-06, Vol.1051, p.104-110</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16126949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiss, Mária</creatorcontrib><creatorcontrib>Perényi, Adám</creatorcontrib><creatorcontrib>Marczinovits, Ilona</creatorcontrib><creatorcontrib>Molnár, János</creatorcontrib><creatorcontrib>Dobozy, Attila</creatorcontrib><creatorcontrib>Kemény, Lajos</creatorcontrib><creatorcontrib>Husz, Sándor</creatorcontrib><title>Autoantibodies to human alpha6 integrin in patients with bullous pemphigoid</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>Bullous pemphigoid (BP) is characterized immunologically by tissue-bound and circulating autoantibodies targeting the hemidesmosomal proteins BP230 and BP180. Recent evidence suggests a pathophysiological role for autoantibodies against alpha6 integrin in the subepidermal blister formation of oral pemphigoid. The objective of our study was to investigate the presence of anti-alpha6 integrin antibodies in patients with classical BP. The autoantibody profiles of 30 patients with BP, 10 patients with pemphigus vulgaris, and 20 healthy persons were identified. With the use of PeptideStructure and PlotStructure software, four different antigenic epitopes for alpha6 integrin were predicted, and their fusion recombinant constructs were prepared in an E. coli expression system. Sera were tested for alpha6 integrin autoantibodies by an ELISA technique. Altogether, 52% of the patients with BP displayed circulating antibodies against at least one recombinant protein. Our findings provide the first evidence for the presence of anti-alpha6 integrin antibodies in patients with classical BP.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Autoantibodies - blood</subject><subject>Female</subject><subject>Humans</subject><subject>Integrin alpha6 - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pemphigoid, Bullous - immunology</subject><issn>0077-8923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jz1PwzAURT2AaCn8BeSJLZLtOH72WFV8iUos3aOX2GmMnMTEjhD_nkiU6d7h6OjeK7JlDKDQRpQbcpvSJ2NcaAk3ZMMVF8pIsyXv-yVPOGbfTNa7RPNE-2XAkWKIPSrqx-zOsx_XQiNm78ac6LfPPW2WEKYl0eiG2Pvz5O0due4wJHd_yR05PT-dDq_F8ePl7bA_FrGSpjDGopUNSugaA85iBYq7skTVAtcCNdedsFpKBlq1RkAFUgLniF2jKsHLHXn808Z5-lpcyvXgU-tCwNGtg2qlK8GEghV8uIBLMzhbx9kPOP_U_-_LX8N6VUU</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>Kiss, Mária</creator><creator>Perényi, Adám</creator><creator>Marczinovits, Ilona</creator><creator>Molnár, János</creator><creator>Dobozy, Attila</creator><creator>Kemény, Lajos</creator><creator>Husz, Sándor</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200506</creationdate><title>Autoantibodies to human alpha6 integrin in patients with bullous pemphigoid</title><author>Kiss, Mária ; Perényi, Adám ; Marczinovits, Ilona ; Molnár, János ; Dobozy, Attila ; Kemény, Lajos ; Husz, Sándor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p549-99dad4ba47fb97eda5761e33a6c7182a818f2d8440786c9275744711aafb65213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Autoantibodies - blood</topic><topic>Female</topic><topic>Humans</topic><topic>Integrin alpha6 - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pemphigoid, Bullous - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiss, Mária</creatorcontrib><creatorcontrib>Perényi, Adám</creatorcontrib><creatorcontrib>Marczinovits, Ilona</creatorcontrib><creatorcontrib>Molnár, János</creatorcontrib><creatorcontrib>Dobozy, Attila</creatorcontrib><creatorcontrib>Kemény, Lajos</creatorcontrib><creatorcontrib>Husz, Sándor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiss, Mária</au><au>Perényi, Adám</au><au>Marczinovits, Ilona</au><au>Molnár, János</au><au>Dobozy, Attila</au><au>Kemény, Lajos</au><au>Husz, Sándor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoantibodies to human alpha6 integrin in patients with bullous pemphigoid</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2005-06</date><risdate>2005</risdate><volume>1051</volume><spage>104</spage><epage>110</epage><pages>104-110</pages><issn>0077-8923</issn><abstract>Bullous pemphigoid (BP) is characterized immunologically by tissue-bound and circulating autoantibodies targeting the hemidesmosomal proteins BP230 and BP180. Recent evidence suggests a pathophysiological role for autoantibodies against alpha6 integrin in the subepidermal blister formation of oral pemphigoid. The objective of our study was to investigate the presence of anti-alpha6 integrin antibodies in patients with classical BP. The autoantibody profiles of 30 patients with BP, 10 patients with pemphigus vulgaris, and 20 healthy persons were identified. With the use of PeptideStructure and PlotStructure software, four different antigenic epitopes for alpha6 integrin were predicted, and their fusion recombinant constructs were prepared in an E. coli expression system. Sera were tested for alpha6 integrin autoantibodies by an ELISA technique. Altogether, 52% of the patients with BP displayed circulating antibodies against at least one recombinant protein. 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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adult Aged Aged, 80 and over Autoantibodies - blood Female Humans Integrin alpha6 - immunology Male Middle Aged Pemphigoid, Bullous - immunology |
| title | Autoantibodies to human alpha6 integrin in patients with bullous pemphigoid |
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