Study of the inhibition of α-amylase by the benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine
Inhibition of porcine pancreas and human saliva α-amylase (EC 3.2.1.1) by sanguinarine and chelerythrine was studied. The inhibition of α-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 μM sanguinarine and 250 μM chelerythrine cause c...
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| Veröffentlicht in: | Journal of enzyme inhibition and medicinal chemistry 2005-06, Vol.20 (3), p.261-267 |
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| container_title | Journal of enzyme inhibition and medicinal chemistry |
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| creator | Zajoncová, Ludmila Kosina, Pavel Vi ar, Jaroslav Ulrichová, Jitka Pe, Pavel |
| description | Inhibition of porcine pancreas and human saliva α-amylase (EC 3.2.1.1) by sanguinarine and chelerythrine was studied. The inhibition of α-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 μM sanguinarine and 250 μM chelerythrine cause complete inhibition of 1.9 nkat α-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat α-amylase from human saliva. Mixed type and partially reversible inhibition was found for both α-amylases treated with either alkaloid. |
| doi_str_mv | 10.1080/14756360500067504 |
| format | Article |
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The inhibition of α-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 μM sanguinarine and 250 μM chelerythrine cause complete inhibition of 1.9 nkat α-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat α-amylase from human saliva. 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The inhibition of α-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 μM sanguinarine and 250 μM chelerythrine cause complete inhibition of 1.9 nkat α-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat α-amylase from human saliva. Mixed type and partially reversible inhibition was found for both α-amylases treated with either alkaloid.</description><subject>Alkaloids - metabolism</subject><subject>Alkaloids - pharmacology</subject><subject>alpha-Amylases - antagonists & inhibitors</subject><subject>alpha-Amylases - metabolism</subject><subject>amperometric biosensor analyser</subject><subject>Animals</subject><subject>Benzophenanthridines</subject><subject>Biosensing Techniques</subject><subject>chelerythrine</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzyme Stability</subject><subject>flow analysis</subject><subject>Humans</subject><subject>Isoquinolines</subject><subject>Kinetics</subject><subject>peroxide electrode</subject><subject>Phenanthridines - metabolism</subject><subject>Phenanthridines - pharmacology</subject><subject>Sanguinarine</subject><subject>Swine</subject><subject>Time Factors</subject><subject>α-amylase inhibition</subject><issn>1475-6366</issn><issn>1475-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtq3jAQhUVJaW59gGyKV9k51Vi2bNNuQkgvEOii7SoUMZbHsVJZ-iPZBPet-iJ9pvq_0BIC_2qGM985DIexM-AXwCv-FvKykELygnMuy4LnL9jRWkulKPODf7uUh-w4xnvOM8ggf8UOQQLUUJdHzH4dp3ZOfJeMPSXG9aYxo_Furfz5neIwW4yUNPPm3pD75W_1j1VPDt3YB9MaRwnan2i9aWMS0d1NxmHYyK5NdE-WwrxGHZ2ylx3aSK9384R9_3D97epTevPl4-ery5tU53k5pplG2XRNKSoBXFIlCwGy0zloXYDIBJYSudCZFmWhm6xAyNsWqiqra6qxqsUJO9_mroJ_mCiOajBRk7XoyE9RyWrJqSu5gLAFdfAxBurUKpgBw6yAq3XF6lnFi-fNLnxqBmr_O3adLsD7LWBc58OAjz7YVo04Wx-6gE6bqMS-_HdP7D2hHXuNgdS9n4Jbitvz3V9raZ3D</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Zajoncová, Ludmila</creator><creator>Kosina, Pavel</creator><creator>Vi ar, Jaroslav</creator><creator>Ulrichová, Jitka</creator><creator>Pe, Pavel</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Study of the inhibition of α-amylase by the benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine</title><author>Zajoncová, Ludmila ; 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The inhibition of α-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 μM sanguinarine and 250 μM chelerythrine cause complete inhibition of 1.9 nkat α-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat α-amylase from human saliva. Mixed type and partially reversible inhibition was found for both α-amylases treated with either alkaloid.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>16119197</pmid><doi>10.1080/14756360500067504</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of enzyme inhibition and medicinal chemistry, 2005-06, Vol.20 (3), p.261-267 |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); EZB-FREE-00999 freely available EZB journals |
| subjects | Alkaloids - metabolism Alkaloids - pharmacology alpha-Amylases - antagonists & inhibitors alpha-Amylases - metabolism amperometric biosensor analyser Animals Benzophenanthridines Biosensing Techniques chelerythrine Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Enzyme Stability flow analysis Humans Isoquinolines Kinetics peroxide electrode Phenanthridines - metabolism Phenanthridines - pharmacology Sanguinarine Swine Time Factors α-amylase inhibition |
| title | Study of the inhibition of α-amylase by the benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine |
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