Use of a competitive probe in assay design for genotyping of the UGT1A1 28 microsatellite polymorphism by the smart amplification process

A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS). However we report here that use of SMAP-2 for polymorphism determination of the UGT1A1 *28 allele re...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques 2007-10, Vol.43 (4), p.479-484
Hauptverfasser:	Watanabe, Jun, Mitani, Yasumasa, Kawai, Yuki, Kikuchi, Takeshi, Kogo, Yasushi, Oguchi-Katayama, Atsuko, Kanamori, Hajime, Usui, Kengo, Itoh, Masayoshi, Cizdziel, Paul E, Lezhava, Alexander, Tatsumi, Kenji, Ichikawa, Yasushi, Togo, Shinji, Shimada, Hiroshi, Hayashizaki, Yoshihide
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Assay - methods DNA Probes - genetics Genotype Glucuronosyltransferase - genetics Microsatellite Repeats - genetics Nucleic Acid Amplification Techniques - methods Polymorphism, Single Nucleotide - genetics Reproducibility of Results Sensitivity and Specificity Sequence Analysis, DNA - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!