Molecular Typing of Methicillin-Resistant Staphylococcus aureus by PCR-RFLP and Its Usefulness in an Epidemiological Study of an Outbreak
A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those pr...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2005/08/29, Vol.58(4), pp.250-252 |
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| container_title | Japanese Journal of Infectious Diseases |
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| creator | Mitani, Noriaki Koizumi, Akira Sano, Reiko Masutani, Takayuki Murakawa, Koichi Mikasa, Keiichi Okamoto, Yasuyuki |
| description | A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those previously reported for sequencing-based spa typing. Twenty-nine isolates of the most frequent protein A (spa) type were discriminated into 6 different types by PCR-RFLP. In contrast, spa typing could discriminate only 1 of the 19 most frequent PCR-RFLP-type isolates. The discriminatory powers of the two methods were equal for the other isolates. These results suggest that PCR-RFLP has the advantages of both relative easiness and greater discriminatory power than spa typing. We also report the case of a suspected outbreak in which PCR-RFLP was sufficient for ruling out the possibility of an outbreak. Thus, PCR-RFLP is preferable as a preliminary screening method for epidemiological studies of nosocomial infection caused by MRSA. |
| doi_str_mv | 10.7883/yoken.JJID.2005.250 |
| format | Article |
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Twenty-nine isolates of the most frequent protein A (spa) type were discriminated into 6 different types by PCR-RFLP. In contrast, spa typing could discriminate only 1 of the 19 most frequent PCR-RFLP-type isolates. The discriminatory powers of the two methods were equal for the other isolates. These results suggest that PCR-RFLP has the advantages of both relative easiness and greater discriminatory power than spa typing. We also report the case of a suspected outbreak in which PCR-RFLP was sufficient for ruling out the possibility of an outbreak. Thus, PCR-RFLP is preferable as a preliminary screening method for epidemiological studies of nosocomial infection caused by MRSA.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.JJID.2005.250</identifier><identifier>PMID: 16116263</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacterial Typing Techniques - methods ; Coagulase - genetics ; Cross Infection - epidemiology ; Cross Infection - microbiology ; Deoxyribonucleases, Type II Site-Specific ; Disease Outbreaks ; Genes, Bacterial - genetics ; Humans ; Japan - epidemiology ; Methicillin - pharmacology ; Methicillin Resistance ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcal Protein A - genetics ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification</subject><ispartof>Japanese Journal of Infectious Diseases, 2005/08/29, Vol.58(4), pp.250-252</ispartof><rights>2005 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-fab2b07ec82acfc85c0774753ed113a1d492ce491effc7e265bfb6221d57196f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16116263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitani, Noriaki</creatorcontrib><creatorcontrib>Koizumi, Akira</creatorcontrib><creatorcontrib>Sano, Reiko</creatorcontrib><creatorcontrib>Masutani, Takayuki</creatorcontrib><creatorcontrib>Murakawa, Koichi</creatorcontrib><creatorcontrib>Mikasa, Keiichi</creatorcontrib><creatorcontrib>Okamoto, Yasuyuki</creatorcontrib><title>Molecular Typing of Methicillin-Resistant Staphylococcus aureus by PCR-RFLP and Its Usefulness in an Epidemiological Study of an Outbreak</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those previously reported for sequencing-based spa typing. Twenty-nine isolates of the most frequent protein A (spa) type were discriminated into 6 different types by PCR-RFLP. In contrast, spa typing could discriminate only 1 of the 19 most frequent PCR-RFLP-type isolates. The discriminatory powers of the two methods were equal for the other isolates. These results suggest that PCR-RFLP has the advantages of both relative easiness and greater discriminatory power than spa typing. We also report the case of a suspected outbreak in which PCR-RFLP was sufficient for ruling out the possibility of an outbreak. Thus, PCR-RFLP is preferable as a preliminary screening method for epidemiological studies of nosocomial infection caused by MRSA.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Coagulase - genetics</subject><subject>Cross Infection - epidemiology</subject><subject>Cross Infection - microbiology</subject><subject>Deoxyribonucleases, Type II Site-Specific</subject><subject>Disease Outbreaks</subject><subject>Genes, Bacterial - genetics</subject><subject>Humans</subject><subject>Japan - epidemiology</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin Resistance</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Staphylococcal Infections - epidemiology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Protein A - genetics</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc9u1DAQhyMEoqXwBEjIJ27Z-k_seI9oaWGrrVot7dlynPGuW28c7OSQR-CtcdhVKy4ey_7mN9J8RfGZ4EUtJbucwjN0i5ub9fcFxZgvKMdvinMiZVVSycTbfGdVVQqGq7PiQ0pPGFPOCX5fnBFBiKCCnRd_boMHM3od0cPUu26HgkW3MOydcd67rtxCcmnQ3YB-DbrfTz6YYMyYkB4j5NJM6H61LbfXm3ukuxath4QeE9jRd5AScl1-RVe9a-Hggg87Z7TPUWM7zZPy3904NBH088findU-wadTvSger68eVj_Lzd2P9erbpjRciqG0uqENrsFIqo01khtc11XNGbSEME3aakkNVEsC1poaqOCNbQSlpOU1WQrLLoqvx9w-ht8jpEEdXDLgve4gjEkJybGklGaQHUETQ0oRrOqjO-g4KYLVbED9M6BmA2o2oLKB3PXlFD82B2hfe04rz8D6CDzlte7gBdBxcMbDKZRLVc3Hf-EvjNnrqKBjfwECl6AR</recordid><startdate>20050829</startdate><enddate>20050829</enddate><creator>Mitani, Noriaki</creator><creator>Koizumi, Akira</creator><creator>Sano, Reiko</creator><creator>Masutani, Takayuki</creator><creator>Murakawa, Koichi</creator><creator>Mikasa, Keiichi</creator><creator>Okamoto, Yasuyuki</creator><general>National Institute of Infectious Diseases</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050829</creationdate><title>Molecular Typing of Methicillin-Resistant Staphylococcus aureus by PCR-RFLP and Its Usefulness in an Epidemiological Study of an Outbreak</title><author>Mitani, Noriaki ; Koizumi, Akira ; Sano, Reiko ; Masutani, Takayuki ; Murakawa, Koichi ; Mikasa, Keiichi ; Okamoto, Yasuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c586t-fab2b07ec82acfc85c0774753ed113a1d492ce491effc7e265bfb6221d57196f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Coagulase - genetics</topic><topic>Cross Infection - epidemiology</topic><topic>Cross Infection - microbiology</topic><topic>Deoxyribonucleases, Type II Site-Specific</topic><topic>Disease Outbreaks</topic><topic>Genes, Bacterial - genetics</topic><topic>Humans</topic><topic>Japan - epidemiology</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin Resistance</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Staphylococcal Infections - epidemiology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Protein A - genetics</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitani, Noriaki</creatorcontrib><creatorcontrib>Koizumi, Akira</creatorcontrib><creatorcontrib>Sano, Reiko</creatorcontrib><creatorcontrib>Masutani, Takayuki</creatorcontrib><creatorcontrib>Murakawa, Koichi</creatorcontrib><creatorcontrib>Mikasa, Keiichi</creatorcontrib><creatorcontrib>Okamoto, Yasuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Infectious Diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitani, Noriaki</au><au>Koizumi, Akira</au><au>Sano, Reiko</au><au>Masutani, Takayuki</au><au>Murakawa, Koichi</au><au>Mikasa, Keiichi</au><au>Okamoto, Yasuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Typing of Methicillin-Resistant Staphylococcus aureus by PCR-RFLP and Its Usefulness in an Epidemiological Study of an Outbreak</atitle><jtitle>Japanese Journal of Infectious Diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2005-08-29</date><risdate>2005</risdate><volume>58</volume><issue>4</issue><spage>250</spage><epage>252</epage><pages>250-252</pages><artnum>JJID.2005.250</artnum><issn>1344-6304</issn><eissn>1884-2836</eissn><abstract>A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those previously reported for sequencing-based spa typing. Twenty-nine isolates of the most frequent protein A (spa) type were discriminated into 6 different types by PCR-RFLP. In contrast, spa typing could discriminate only 1 of the 19 most frequent PCR-RFLP-type isolates. The discriminatory powers of the two methods were equal for the other isolates. These results suggest that PCR-RFLP has the advantages of both relative easiness and greater discriminatory power than spa typing. We also report the case of a suspected outbreak in which PCR-RFLP was sufficient for ruling out the possibility of an outbreak. Thus, PCR-RFLP is preferable as a preliminary screening method for epidemiological studies of nosocomial infection caused by MRSA.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases</pub><pmid>16116263</pmid><doi>10.7883/yoken.JJID.2005.250</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Bacterial Typing Techniques - methods Coagulase - genetics Cross Infection - epidemiology Cross Infection - microbiology Deoxyribonucleases, Type II Site-Specific Disease Outbreaks Genes, Bacterial - genetics Humans Japan - epidemiology Methicillin - pharmacology Methicillin Resistance Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Staphylococcal Infections - epidemiology Staphylococcal Infections - microbiology Staphylococcal Protein A - genetics Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification |
| title | Molecular Typing of Methicillin-Resistant Staphylococcus aureus by PCR-RFLP and Its Usefulness in an Epidemiological Study of an Outbreak |
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