Versatile, Fully Automated, Microfluidic Cell Culture System
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture me...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2007-11, Vol.79 (22), p.8557-8563 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Gómez-Sjöberg, Rafael Leyrat, Anne A Pirone, Dana M Chen, Christopher S Quake, Stephen R |
| description | There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation, osteogenic differentiation, and motility of human primary mesenchymal stem cells. |
| doi_str_mv | 10.1021/ac071311w |
| format | Article |
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Hybridization. Fusion</topic><topic>Cell Differentiation</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. 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| source | American Chemical Society; MEDLINE |
| subjects | Analytical chemistry Automation Biological and medical sciences Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell cultures. Hybridization. Fusion Cell Differentiation Cell Movement Cell Proliferation Cells, Cultured Fundamental and applied biological sciences. Psychology General aspects Humans Mesenchymal Stromal Cells - cytology Microfluidics - instrumentation Microfluidics - methods Microscopy Molecular and cellular biology Stem cells |
| title | Versatile, Fully Automated, Microfluidic Cell Culture System |
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