Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)

The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant spe...

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Veröffentlicht in:Systematic and applied microbiology 2007-12, Vol.30 (8), p.601-614
Hauptverfasser: García-Moyano, A., González-Toril, E., Aguilera, A., Amils, R.
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container_issue 8
container_start_page 601
container_title Systematic and applied microbiology
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creator García-Moyano, A.
González-Toril, E.
Aguilera, A.
Amils, R.
description The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera Aeromonas and Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera Desulfosporosinus, Clostridium and Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.
doi_str_mv 10.1016/j.syapm.2007.08.002
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subjects Acidiphilium
Acidithiobacillus ferrooxidans
Acidophiles
Acinetobacter
Aeromonas
Animal, plant and microbial ecology
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
Biological and medical sciences
Clostridium
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Electrophoresis, Polyacrylamide Gel
Extremophiles
Freshwater
Fundamental and applied biological sciences. Psychology
Genes, rRNA
In Situ Hybridization, Fluorescence
Leptospirillum ferrooxidans
Macroscopic filaments
Microbial ecology
Molecular ecology
Molecular Sequence Data
Mycobacterium
Nucleic Acid Denaturation
Phylogeny
Rivers - microbiology
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Río Tinto
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spain
Various environments (extraatmospheric space, air, water)
title Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)
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