Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)
The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant spe...
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| Veröffentlicht in: | Systematic and applied microbiology 2007-12, Vol.30 (8), p.601-614 |
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| creator | García-Moyano, A. González-Toril, E. Aguilera, A. Amils, R. |
| description | The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were
Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and
Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the
Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera
Aeromonas and
Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera
Desulfosporosinus,
Clostridium and
Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy. |
| doi_str_mv | 10.1016/j.syapm.2007.08.002 |
| format | Article |
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Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and
Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the
Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera
Aeromonas and
Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera
Desulfosporosinus,
Clostridium and
Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2007.08.002</identifier><identifier>PMID: 17950555</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Acidiphilium ; Acidithiobacillus ferrooxidans ; Acidophiles ; Acinetobacter ; Aeromonas ; Animal, plant and microbial ecology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Biodiversity ; Biological and medical sciences ; Clostridium ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Electrophoresis, Polyacrylamide Gel ; Extremophiles ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; In Situ Hybridization, Fluorescence ; Leptospirillum ferrooxidans ; Macroscopic filaments ; Microbial ecology ; Molecular ecology ; Molecular Sequence Data ; Mycobacterium ; Nucleic Acid Denaturation ; Phylogeny ; Rivers - microbiology ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Río Tinto ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Spain ; Various environments (extraatmospheric space, air, water)</subject><ispartof>Systematic and applied microbiology, 2007-12, Vol.30 (8), p.601-614</ispartof><rights>2007 Elsevier GmbH</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</citedby><cites>FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2007.08.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19865440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17950555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Moyano, A.</creatorcontrib><creatorcontrib>González-Toril, E.</creatorcontrib><creatorcontrib>Aguilera, A.</creatorcontrib><creatorcontrib>Amils, R.</creatorcontrib><title>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were
Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and
Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the
Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera
Aeromonas and
Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera
Desulfosporosinus,
Clostridium and
Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</description><subject>Acidiphilium</subject><subject>Acidithiobacillus ferrooxidans</subject><subject>Acidophiles</subject><subject>Acinetobacter</subject><subject>Aeromonas</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Clostridium</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Extremophiles</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Leptospirillum ferrooxidans</subject><subject>Macroscopic filaments</subject><subject>Microbial ecology</subject><subject>Molecular ecology</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium</subject><subject>Nucleic Acid Denaturation</subject><subject>Phylogeny</subject><subject>Rivers - microbiology</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Río Tinto</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Spain</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMoTjv6BIJkoyhMlyd1SaUWLmTwBgOKM-IypJOTIW1VUibpwX4F38Wn8MVM2Q2z09XJge8_hP8j5DGDigHjL7dV2qt5qmqAvgJRAdR3yIpxJtYwiPYuWUFfN-saajghD1LaArB24Ow-OWH90EHXdSvy81MM31Tch-w01WGadt7l_fKaQ3LZBU-VNxR1GMP1ngZL7RhUdv6aTkrHkHSYS9K6UU3oc6I2hqlEKP7IESekSjtTAPQ3Lga_MGf08-9fgV45nwN9fvn1jF7OyvkXD8k9q8aEj47zlHx5--bq_P364uO7D-evL9a6ZSKvrVYMNwzQ9r0ZysbZ0GLX1hvOGquN4ZrbujEogBvTFHpTs0ZrIRoDVtnmlDw73J1j-L7DlOXkksZxVB7DLkkuOoDSzn_BGkTbNn1fwOYALoWkiFbO0U2lVclALq7kVv51JRdXEoQsrkrqyfH8bjOhuc0c5RTg6RFQSavRRuW1S7fcIHjXtlC4VwcOS2s3DqNM2qHXaFxEnaUJ7p8f-QMs8LeP</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>García-Moyano, A.</creator><creator>González-Toril, E.</creator><creator>Aguilera, A.</creator><creator>Amils, R.</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</title><author>García-Moyano, A. ; González-Toril, E. ; Aguilera, A. ; Amils, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acidiphilium</topic><topic>Acidithiobacillus ferrooxidans</topic><topic>Acidophiles</topic><topic>Acinetobacter</topic><topic>Aeromonas</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Clostridium</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Extremophiles</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Leptospirillum ferrooxidans</topic><topic>Macroscopic filaments</topic><topic>Microbial ecology</topic><topic>Molecular ecology</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium</topic><topic>Nucleic Acid Denaturation</topic><topic>Phylogeny</topic><topic>Rivers - microbiology</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Río Tinto</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Spain</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Moyano, A.</creatorcontrib><creatorcontrib>González-Toril, E.</creatorcontrib><creatorcontrib>Aguilera, A.</creatorcontrib><creatorcontrib>Amils, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Moyano, A.</au><au>González-Toril, E.</au><au>Aguilera, A.</au><au>Amils, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>30</volume><issue>8</issue><spage>601</spage><epage>614</epage><pages>601-614</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were
Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and
Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the
Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera
Aeromonas and
Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera
Desulfosporosinus,
Clostridium and
Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>17950555</pmid><doi>10.1016/j.syapm.2007.08.002</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Systematic and applied microbiology, 2007-12, Vol.30 (8), p.601-614 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acidiphilium Acidithiobacillus ferrooxidans Acidophiles Acinetobacter Aeromonas Animal, plant and microbial ecology Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biological and medical sciences Clostridium DNA Fingerprinting DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Electrophoresis, Polyacrylamide Gel Extremophiles Freshwater Fundamental and applied biological sciences. Psychology Genes, rRNA In Situ Hybridization, Fluorescence Leptospirillum ferrooxidans Macroscopic filaments Microbial ecology Molecular ecology Molecular Sequence Data Mycobacterium Nucleic Acid Denaturation Phylogeny Rivers - microbiology RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Río Tinto Sequence Analysis, DNA Sequence Homology, Nucleic Acid Spain Various environments (extraatmospheric space, air, water) |
| title | Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain) |
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