Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)

The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant spe...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Systematic and applied microbiology 2007-12, Vol.30 (8), p.601-614
Hauptverfasser: García-Moyano, A., González-Toril, E., Aguilera, A., Amils, R.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 614
container_issue 8
container_start_page 601
container_title Systematic and applied microbiology
container_volume 30
creator García-Moyano, A.
González-Toril, E.
Aguilera, A.
Amils, R.
description The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera Aeromonas and Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera Desulfosporosinus, Clostridium and Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.
doi_str_mv 10.1016/j.syapm.2007.08.002
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68500055</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0723202007001014</els_id><sourcerecordid>68500055</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</originalsourceid><addsrcrecordid>eNqFkcuKFDEUhoMoTjv6BIJkoyhMlyd1SaUWLmTwBgOKM-IypJOTIW1VUibpwX4F38Wn8MVM2Q2z09XJge8_hP8j5DGDigHjL7dV2qt5qmqAvgJRAdR3yIpxJtYwiPYuWUFfN-saajghD1LaArB24Ow-OWH90EHXdSvy81MM31Tch-w01WGadt7l_fKaQ3LZBU-VNxR1GMP1ngZL7RhUdv6aTkrHkHSYS9K6UU3oc6I2hqlEKP7IESekSjtTAPQ3Lga_MGf08-9fgV45nwN9fvn1jF7OyvkXD8k9q8aEj47zlHx5--bq_P364uO7D-evL9a6ZSKvrVYMNwzQ9r0ZysbZ0GLX1hvOGquN4ZrbujEogBvTFHpTs0ZrIRoDVtnmlDw73J1j-L7DlOXkksZxVB7DLkkuOoDSzn_BGkTbNn1fwOYALoWkiFbO0U2lVclALq7kVv51JRdXEoQsrkrqyfH8bjOhuc0c5RTg6RFQSavRRuW1S7fcIHjXtlC4VwcOS2s3DqNM2qHXaFxEnaUJ7p8f-QMs8LeP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20844377</pqid></control><display><type>article</type><title>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>García-Moyano, A. ; González-Toril, E. ; Aguilera, A. ; Amils, R.</creator><creatorcontrib>García-Moyano, A. ; González-Toril, E. ; Aguilera, A. ; Amils, R.</creatorcontrib><description>The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera Aeromonas and Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera Desulfosporosinus, Clostridium and Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2007.08.002</identifier><identifier>PMID: 17950555</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Acidiphilium ; Acidithiobacillus ferrooxidans ; Acidophiles ; Acinetobacter ; Aeromonas ; Animal, plant and microbial ecology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation &amp; purification ; Biodiversity ; Biological and medical sciences ; Clostridium ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation &amp; purification ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Electrophoresis, Polyacrylamide Gel ; Extremophiles ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; In Situ Hybridization, Fluorescence ; Leptospirillum ferrooxidans ; Macroscopic filaments ; Microbial ecology ; Molecular ecology ; Molecular Sequence Data ; Mycobacterium ; Nucleic Acid Denaturation ; Phylogeny ; Rivers - microbiology ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Río Tinto ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Spain ; Various environments (extraatmospheric space, air, water)</subject><ispartof>Systematic and applied microbiology, 2007-12, Vol.30 (8), p.601-614</ispartof><rights>2007 Elsevier GmbH</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</citedby><cites>FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.syapm.2007.08.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=19865440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17950555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Moyano, A.</creatorcontrib><creatorcontrib>González-Toril, E.</creatorcontrib><creatorcontrib>Aguilera, A.</creatorcontrib><creatorcontrib>Amils, R.</creatorcontrib><title>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera Aeromonas and Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera Desulfosporosinus, Clostridium and Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</description><subject>Acidiphilium</subject><subject>Acidithiobacillus ferrooxidans</subject><subject>Acidophiles</subject><subject>Acinetobacter</subject><subject>Aeromonas</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation &amp; purification</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Clostridium</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation &amp; purification</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Extremophiles</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Leptospirillum ferrooxidans</subject><subject>Macroscopic filaments</subject><subject>Microbial ecology</subject><subject>Molecular ecology</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium</subject><subject>Nucleic Acid Denaturation</subject><subject>Phylogeny</subject><subject>Rivers - microbiology</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Río Tinto</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Spain</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMoTjv6BIJkoyhMlyd1SaUWLmTwBgOKM-IypJOTIW1VUibpwX4F38Wn8MVM2Q2z09XJge8_hP8j5DGDigHjL7dV2qt5qmqAvgJRAdR3yIpxJtYwiPYuWUFfN-saajghD1LaArB24Ow-OWH90EHXdSvy81MM31Tch-w01WGadt7l_fKaQ3LZBU-VNxR1GMP1ngZL7RhUdv6aTkrHkHSYS9K6UU3oc6I2hqlEKP7IESekSjtTAPQ3Lga_MGf08-9fgV45nwN9fvn1jF7OyvkXD8k9q8aEj47zlHx5--bq_P364uO7D-evL9a6ZSKvrVYMNwzQ9r0ZysbZ0GLX1hvOGquN4ZrbujEogBvTFHpTs0ZrIRoDVtnmlDw73J1j-L7DlOXkksZxVB7DLkkuOoDSzn_BGkTbNn1fwOYALoWkiFbO0U2lVclALq7kVv51JRdXEoQsrkrqyfH8bjOhuc0c5RTg6RFQSavRRuW1S7fcIHjXtlC4VwcOS2s3DqNM2qHXaFxEnaUJ7p8f-QMs8LeP</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>García-Moyano, A.</creator><creator>González-Toril, E.</creator><creator>Aguilera, A.</creator><creator>Amils, R.</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</title><author>García-Moyano, A. ; González-Toril, E. ; Aguilera, A. ; Amils, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-fca1eb10ef77d9fca6194e542b613fcdd6c6f23de806dd3ca1b213cc883d0faf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acidiphilium</topic><topic>Acidithiobacillus ferrooxidans</topic><topic>Acidophiles</topic><topic>Acinetobacter</topic><topic>Aeromonas</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation &amp; purification</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Clostridium</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation &amp; purification</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Extremophiles</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Leptospirillum ferrooxidans</topic><topic>Macroscopic filaments</topic><topic>Microbial ecology</topic><topic>Molecular ecology</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium</topic><topic>Nucleic Acid Denaturation</topic><topic>Phylogeny</topic><topic>Rivers - microbiology</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Río Tinto</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Spain</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Moyano, A.</creatorcontrib><creatorcontrib>González-Toril, E.</creatorcontrib><creatorcontrib>Aguilera, A.</creatorcontrib><creatorcontrib>Amils, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Moyano, A.</au><au>González-Toril, E.</au><au>Aguilera, A.</au><au>Amils, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>30</volume><issue>8</issue><spage>601</spage><epage>614</epage><pages>601-614</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>The prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, Río Tinto (SW, Spain), has been analysed through denaturing gradient gel electrophoresis (DGGE), cloning of 16S rRNA genes and fluorescence in situ hybridization (FISH). The dominant species present in the macrofilaments were Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans and Acidiphilium spp., which represented the three main bacterial genera found in the water column of the river. However, our results also showed the presence of other microorganisms not previously detected in the Río Tinto. Within the Gammaproteobacteria class, sequences closely related to the iron-oxidizing bacteria WJ2 and DSM 2392 were found, as well as sequences related to the non-acidophilic genera Aeromonas and Acinetobacter. In addition, two other new phylotypes related to Gram-positive species from the genera Desulfosporosinus, Clostridium and Mycobacterium were identified. The presence of these anaerobic microorganisms strongly suggests that the filaments could originate in the deeper parts of the river as a typical acid streamer attached to the rocks or the sediments and be pulled up towards the surface when they reach certain buoyancy.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>17950555</pmid><doi>10.1016/j.syapm.2007.08.002</doi><tpages>14</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0723-2020
ispartof Systematic and applied microbiology, 2007-12, Vol.30 (8), p.601-614
issn 0723-2020
1618-0984
language eng
recordid cdi_proquest_miscellaneous_68500055
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Acidiphilium
Acidithiobacillus ferrooxidans
Acidophiles
Acinetobacter
Aeromonas
Animal, plant and microbial ecology
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
Biological and medical sciences
Clostridium
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Electrophoresis, Polyacrylamide Gel
Extremophiles
Freshwater
Fundamental and applied biological sciences. Psychology
Genes, rRNA
In Situ Hybridization, Fluorescence
Leptospirillum ferrooxidans
Macroscopic filaments
Microbial ecology
Molecular ecology
Molecular Sequence Data
Mycobacterium
Nucleic Acid Denaturation
Phylogeny
Rivers - microbiology
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Río Tinto
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spain
Various environments (extraatmospheric space, air, water)
title Prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, Río Tinto (SW, Spain)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T18%3A21%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prokaryotic%20community%20composition%20and%20ecology%20of%20floating%20macroscopic%20filaments%20from%20an%20extreme%20acidic%20environment,%20R%C3%ADo%20Tinto%20(SW,%20Spain)&rft.jtitle=Systematic%20and%20applied%20microbiology&rft.au=Garc%C3%ADa-Moyano,%20A.&rft.date=2007-12-01&rft.volume=30&rft.issue=8&rft.spage=601&rft.epage=614&rft.pages=601-614&rft.issn=0723-2020&rft.eissn=1618-0984&rft.coden=SAMIDF&rft_id=info:doi/10.1016/j.syapm.2007.08.002&rft_dat=%3Cproquest_cross%3E68500055%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20844377&rft_id=info:pmid/17950555&rft_els_id=S0723202007001014&rfr_iscdi=true