Inhibition of bacteriophage K proliferation on Staphylococcus aureus in raw bovine milk
Aims: To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. Methods and Results: The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to...
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Veröffentlicht in: | Letters in applied microbiology 2005-01, Vol.41 (3), p.274-279 |
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creator | O'Flaherty, S Coffey, A Meaney, W.J Fitzgerald, G.F Ross, R.P |
description | Aims: To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk.
Methods and Results: The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, leading to elimination of the host culture in milk, which had been previously heat‐treated. When raw milk was used under identical conditions, the phages were unable to replicate. Phage adsorption assays were performed and these demonstrated that adsorption of phage was significantly reduced in the raw milk while it was restored in the heat‐treated sample (86·50% compared with 99·96% adsorption respectively). When confocal microscopy with a Live/Dead Bac light staining system was employed, it was observed that in raw milk S. aureus formed clusters associated with fat globules, while in heat‐treated milk, bacterial agglutination had not occurred.
Conclusions: Raw milk inhibits staphylococcal phage K proliferation.
Significance and Impact of the Study: This observation has implications for the exploitation of staphylococcal therapeutic phage in milk. |
doi_str_mv | 10.1111/j.1472-765X.2005.01762.x |
format | Article |
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Methods and Results: The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, leading to elimination of the host culture in milk, which had been previously heat‐treated. When raw milk was used under identical conditions, the phages were unable to replicate. Phage adsorption assays were performed and these demonstrated that adsorption of phage was significantly reduced in the raw milk while it was restored in the heat‐treated sample (86·50% compared with 99·96% adsorption respectively). When confocal microscopy with a Live/Dead Bac light staining system was employed, it was observed that in raw milk S. aureus formed clusters associated with fat globules, while in heat‐treated milk, bacterial agglutination had not occurred.
Conclusions: Raw milk inhibits staphylococcal phage K proliferation.
Significance and Impact of the Study: This observation has implications for the exploitation of staphylococcal therapeutic phage in milk.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1111/j.1472-765X.2005.01762.x</identifier><identifier>PMID: 16108920</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; bacterial contamination ; bacteriophage therapy ; bacteriophages ; Biological and medical sciences ; bovine mastitis ; Cattle ; dairy cows ; food contamination ; Fundamental and applied biological sciences. Psychology ; Hot Temperature ; mastitis ; Microbiology ; milk ; Milk - cytology ; Milk - microbiology ; Milk - virology ; phage therapy ; raw milk ; Staphylococcus ; Staphylococcus aureus ; Staphylococcus aureus - virology ; Staphylococcus Phages - physiology ; therapeutics ; Virus Replication</subject><ispartof>Letters in applied microbiology, 2005-01, Vol.41 (3), p.274-279</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5182-1a1249f767ddae5c42d6597cda6a655b602e1cf9500bc11fe0b7b4548c4e14353</citedby><cites>FETCH-LOGICAL-c5182-1a1249f767ddae5c42d6597cda6a655b602e1cf9500bc11fe0b7b4548c4e14353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2005.01762.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2005.01762.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17018486$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16108920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Flaherty, S</creatorcontrib><creatorcontrib>Coffey, A</creatorcontrib><creatorcontrib>Meaney, W.J</creatorcontrib><creatorcontrib>Fitzgerald, G.F</creatorcontrib><creatorcontrib>Ross, R.P</creatorcontrib><title>Inhibition of bacteriophage K proliferation on Staphylococcus aureus in raw bovine milk</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Aims: To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk.
Methods and Results: The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, leading to elimination of the host culture in milk, which had been previously heat‐treated. When raw milk was used under identical conditions, the phages were unable to replicate. Phage adsorption assays were performed and these demonstrated that adsorption of phage was significantly reduced in the raw milk while it was restored in the heat‐treated sample (86·50% compared with 99·96% adsorption respectively). When confocal microscopy with a Live/Dead Bac light staining system was employed, it was observed that in raw milk S. aureus formed clusters associated with fat globules, while in heat‐treated milk, bacterial agglutination had not occurred.
Conclusions: Raw milk inhibits staphylococcal phage K proliferation.
Significance and Impact of the Study: This observation has implications for the exploitation of staphylococcal therapeutic phage in milk.</description><subject>Animals</subject><subject>bacterial contamination</subject><subject>bacteriophage therapy</subject><subject>bacteriophages</subject><subject>Biological and medical sciences</subject><subject>bovine mastitis</subject><subject>Cattle</subject><subject>dairy cows</subject><subject>food contamination</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hot Temperature</subject><subject>mastitis</subject><subject>Microbiology</subject><subject>milk</subject><subject>Milk - cytology</subject><subject>Milk - microbiology</subject><subject>Milk - virology</subject><subject>phage therapy</subject><subject>raw milk</subject><subject>Staphylococcus</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - virology</subject><subject>Staphylococcus Phages - physiology</subject><subject>therapeutics</subject><subject>Virus Replication</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhi0EotvCK4AvcEuYcWzHOXCoKigViziUCm6W43W6XrLx1t7Q7tvjkBU9gi9jab7fM_pkQihCifm825TIa1bUUvwoGYAoAWvJyocnZPG38ZQsgElZKCb4CTlNaQMAClnznJygRFANgwX5fjWsfev3Pgw0dLQ1du-iD7u1uXX0M93F0PvORTMDA73em9360AcbrB0TNWN0ufiBRnNP2_DLD45uff_zBXnWmT65l8d6Rm4-fvh28alYfr28ujhfFlagYgUaZLzpalmvVsYJy9lKiqa2KyONFKKVwBzarhEArUXsHLR1ywVXljvklajOyNv53bzp3ejSXm99sq7vzeDCmLRUvKkrxf4JYi0EMFQZVDNoY0gpuk7vot-aeNAIerKvN3qSrCfJerKv_9jXDzn66jhjbLdu9Rg86s7AmyNgkjV9F81gfXrkakDFlczc-5m79707_PcCenn-Zbrl_Os535mgzW3MM26uGWAFmP8E51D9BtMhqcE</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>O'Flaherty, S</creator><creator>Coffey, A</creator><creator>Meaney, W.J</creator><creator>Fitzgerald, G.F</creator><creator>Ross, R.P</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050101</creationdate><title>Inhibition of bacteriophage K proliferation on Staphylococcus aureus in raw bovine milk</title><author>O'Flaherty, S ; Coffey, A ; Meaney, W.J ; Fitzgerald, G.F ; Ross, R.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5182-1a1249f767ddae5c42d6597cda6a655b602e1cf9500bc11fe0b7b4548c4e14353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>bacterial contamination</topic><topic>bacteriophage therapy</topic><topic>bacteriophages</topic><topic>Biological and medical sciences</topic><topic>bovine mastitis</topic><topic>Cattle</topic><topic>dairy cows</topic><topic>food contamination</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hot Temperature</topic><topic>mastitis</topic><topic>Microbiology</topic><topic>milk</topic><topic>Milk - cytology</topic><topic>Milk - microbiology</topic><topic>Milk - virology</topic><topic>phage therapy</topic><topic>raw milk</topic><topic>Staphylococcus</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - virology</topic><topic>Staphylococcus Phages - physiology</topic><topic>therapeutics</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Flaherty, S</creatorcontrib><creatorcontrib>Coffey, A</creatorcontrib><creatorcontrib>Meaney, W.J</creatorcontrib><creatorcontrib>Fitzgerald, G.F</creatorcontrib><creatorcontrib>Ross, R.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Flaherty, S</au><au>Coffey, A</au><au>Meaney, W.J</au><au>Fitzgerald, G.F</au><au>Ross, R.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of bacteriophage K proliferation on Staphylococcus aureus in raw bovine milk</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>41</volume><issue>3</issue><spage>274</spage><epage>279</epage><pages>274-279</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Aims: To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk.
Methods and Results: The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, leading to elimination of the host culture in milk, which had been previously heat‐treated. When raw milk was used under identical conditions, the phages were unable to replicate. Phage adsorption assays were performed and these demonstrated that adsorption of phage was significantly reduced in the raw milk while it was restored in the heat‐treated sample (86·50% compared with 99·96% adsorption respectively). When confocal microscopy with a Live/Dead Bac light staining system was employed, it was observed that in raw milk S. aureus formed clusters associated with fat globules, while in heat‐treated milk, bacterial agglutination had not occurred.
Conclusions: Raw milk inhibits staphylococcal phage K proliferation.
Significance and Impact of the Study: This observation has implications for the exploitation of staphylococcal therapeutic phage in milk.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16108920</pmid><doi>10.1111/j.1472-765X.2005.01762.x</doi><tpages>6</tpages></addata></record> |
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source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Animals bacterial contamination bacteriophage therapy bacteriophages Biological and medical sciences bovine mastitis Cattle dairy cows food contamination Fundamental and applied biological sciences. Psychology Hot Temperature mastitis Microbiology milk Milk - cytology Milk - microbiology Milk - virology phage therapy raw milk Staphylococcus Staphylococcus aureus Staphylococcus aureus - virology Staphylococcus Phages - physiology therapeutics Virus Replication |
title | Inhibition of bacteriophage K proliferation on Staphylococcus aureus in raw bovine milk |
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