Pituitary Adenylate Cyclase Activating Polypeptide Messenger RNA in the Paraventricular Nucleus and Anterior Pituitary During the Rat Estrous Cycle
The neuropeptide pituitary adenylate cyclase activating polypeptide (ADCYAP 1, or PACAP) has been demonstrated to enhance gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulate gonadotropin subunit gene expression in cultures of anterior pituitary cells. In the present stu...
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| Veröffentlicht in: | Biology of reproduction 2005-09, Vol.73 (3), p.491-499 |
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| creator | MOORE, Joseph P BURGER, Laura L DALKIN, Alan C WINTERS, Stephen J |
| description | The neuropeptide pituitary adenylate cyclase activating polypeptide (ADCYAP 1, or PACAP) has been demonstrated to enhance
gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulate gonadotropin subunit gene expression in
cultures of anterior pituitary cells. In the present study, we used in situ hybridization and real-time polymerase chain reaction
to examine the expression of Pacap mRNA within the paraventricular nucleus (PVN) and anterior pituitary throughout the estrous cycle of the rat. Levels of luteinizing
hormone in serum and pituitary gonadotropin subunit mRNAs were evaluated and displayed cyclic fluctuations similar to those
reported previously. Pacap mRNA expression in the PVN and pituitary varied significantly during the estrous cycle, with the greatest changes occurring
on the day of proestrus. Pacap mRNA levels in the PVN declined significantly on the morning of diestrus. During proestrus, PVN Pacap mRNA levels significantly increased 3 h before the gonadotropin surge and then declined. Pituitary expression of Pacap mRNA also varied on the afternoon of proestrus with a moderate decline at the time of the gonadotropin surge and a significant
increase later in the evening. Expression of the mRNA species encoding the 288 amino acid form of follistatin increased significantly
following the rise in pituitary Pacap mRNA, at the termination of the secondary surge in follicle-stimulating hormone beta ( Fshb ) gene expression. These results suggest that PACAP is involved in events before and following the gonadotropin surge, perhaps
through increased gonadotroph sensitivity to GnRH and suppression of Fshb subunit expression through increased follistatin, as previously observed in vitro.
Abstract
In the rat, pituitary adenylate cyclase-activating polypeptide expression in the paraventricular nucleus and pituitary varies
during the estrous cycle and is correlated with gonadotropin synthesis and secretion |
| doi_str_mv | 10.1095/biolreprod.105.041624 |
| format | Article |
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gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulate gonadotropin subunit gene expression in
cultures of anterior pituitary cells. In the present study, we used in situ hybridization and real-time polymerase chain reaction
to examine the expression of Pacap mRNA within the paraventricular nucleus (PVN) and anterior pituitary throughout the estrous cycle of the rat. Levels of luteinizing
hormone in serum and pituitary gonadotropin subunit mRNAs were evaluated and displayed cyclic fluctuations similar to those
reported previously. Pacap mRNA expression in the PVN and pituitary varied significantly during the estrous cycle, with the greatest changes occurring
on the day of proestrus. Pacap mRNA levels in the PVN declined significantly on the morning of diestrus. During proestrus, PVN Pacap mRNA levels significantly increased 3 h before the gonadotropin surge and then declined. Pituitary expression of Pacap mRNA also varied on the afternoon of proestrus with a moderate decline at the time of the gonadotropin surge and a significant
increase later in the evening. Expression of the mRNA species encoding the 288 amino acid form of follistatin increased significantly
following the rise in pituitary Pacap mRNA, at the termination of the secondary surge in follicle-stimulating hormone beta ( Fshb ) gene expression. These results suggest that PACAP is involved in events before and following the gonadotropin surge, perhaps
through increased gonadotroph sensitivity to GnRH and suppression of Fshb subunit expression through increased follistatin, as previously observed in vitro.
Abstract
In the rat, pituitary adenylate cyclase-activating polypeptide expression in the paraventricular nucleus and pituitary varies
during the estrous cycle and is correlated with gonadotropin synthesis and secretion</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.105.041624</identifier><identifier>PMID: 15917345</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Circadian Rhythm ; Estrous Cycle - metabolism ; Female ; Follicle Stimulating Hormone, beta Subunit - metabolism ; Follistatin - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Gonadotropin-Releasing Hormone - physiology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Nerve Growth Factors - biosynthesis ; Neuropeptides - biosynthesis ; Neurotransmitter Agents - biosynthesis ; Paraventricular Hypothalamic Nucleus - metabolism ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Pituitary Gland, Anterior - metabolism ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - metabolism ; Vertebrates: endocrinology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2005-09, Vol.73 (3), p.491-499</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17062541$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15917345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOORE, Joseph P</creatorcontrib><creatorcontrib>BURGER, Laura L</creatorcontrib><creatorcontrib>DALKIN, Alan C</creatorcontrib><creatorcontrib>WINTERS, Stephen J</creatorcontrib><title>Pituitary Adenylate Cyclase Activating Polypeptide Messenger RNA in the Paraventricular Nucleus and Anterior Pituitary During the Rat Estrous Cycle</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The neuropeptide pituitary adenylate cyclase activating polypeptide (ADCYAP 1, or PACAP) has been demonstrated to enhance
gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulate gonadotropin subunit gene expression in
cultures of anterior pituitary cells. In the present study, we used in situ hybridization and real-time polymerase chain reaction
to examine the expression of Pacap mRNA within the paraventricular nucleus (PVN) and anterior pituitary throughout the estrous cycle of the rat. Levels of luteinizing
hormone in serum and pituitary gonadotropin subunit mRNAs were evaluated and displayed cyclic fluctuations similar to those
reported previously. Pacap mRNA expression in the PVN and pituitary varied significantly during the estrous cycle, with the greatest changes occurring
on the day of proestrus. Pacap mRNA levels in the PVN declined significantly on the morning of diestrus. During proestrus, PVN Pacap mRNA levels significantly increased 3 h before the gonadotropin surge and then declined. Pituitary expression of Pacap mRNA also varied on the afternoon of proestrus with a moderate decline at the time of the gonadotropin surge and a significant
increase later in the evening. Expression of the mRNA species encoding the 288 amino acid form of follistatin increased significantly
following the rise in pituitary Pacap mRNA, at the termination of the secondary surge in follicle-stimulating hormone beta ( Fshb ) gene expression. These results suggest that PACAP is involved in events before and following the gonadotropin surge, perhaps
through increased gonadotroph sensitivity to GnRH and suppression of Fshb subunit expression through increased follistatin, as previously observed in vitro.
Abstract
In the rat, pituitary adenylate cyclase-activating polypeptide expression in the paraventricular nucleus and pituitary varies
during the estrous cycle and is correlated with gonadotropin synthesis and secretion</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Circadian Rhythm</subject><subject>Estrous Cycle - metabolism</subject><subject>Female</subject><subject>Follicle Stimulating Hormone, beta Subunit - metabolism</subject><subject>Follistatin - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Gonadotropin-Releasing Hormone - physiology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Nerve Growth Factors - biosynthesis</subject><subject>Neuropeptides - biosynthesis</subject><subject>Neurotransmitter Agents - biosynthesis</subject><subject>Paraventricular Hypothalamic Nucleus - metabolism</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates: endocrinology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN2O0zAQhS0EYsvCI4B8A3dZxv_NZVSWH2lZqhVcR04yaY1cp9jOVn0OXhhXFPWSi9FopG_OmTmEvGZww6BW7zs3-Yj7OA1lVjcgmebyCVkwxevKcL18ShYAoCshtLgiL1L6CcCk4OI5uWKqZkZItSC_1y7PLtt4pM2A4ehtRro69t4mpE2f3aPNLmzoevLHPe6zG5B-xZQwbDDSh_uGukDzFunaRvuIIUfXz95Gej_3HudEbRhoEzJGN0V6Mfswx5PsafPBZnqbcpwKfXLGl-TZaH3CV-d-TX58vP2--lzdffv0ZdXcVVsBkKtB6Y5zo7uad8ClkGMPujM9GIMWbAdSLBWrBywFAoYS0DjqpWaj1tpYI67Ju7-6JcVfM6bc7lzq0XsbsBzT6qWspTH8vyAzShnNVAHfnMG52-HQ7qPblW_bf3kX4O0ZsKm3fow29C5dOAOaK8ku3NZttgcXsU07632RFe3hcDCiFa2smfgDoKWghA</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>MOORE, Joseph P</creator><creator>BURGER, Laura L</creator><creator>DALKIN, Alan C</creator><creator>WINTERS, Stephen J</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Pituitary Adenylate Cyclase Activating Polypeptide Messenger RNA in the Paraventricular Nucleus and Anterior Pituitary During the Rat Estrous Cycle</title><author>MOORE, Joseph P ; BURGER, Laura L ; DALKIN, Alan C ; WINTERS, Stephen J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h300t-d56b2276b92b02434fc06b7c077ea0ab0438519de19d030d162ff6861f6667a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Circadian Rhythm</topic><topic>Estrous Cycle - metabolism</topic><topic>Female</topic><topic>Follicle Stimulating Hormone, beta Subunit - metabolism</topic><topic>Follistatin - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Gonadotropin-Releasing Hormone - physiology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Nerve Growth Factors - biosynthesis</topic><topic>Neuropeptides - biosynthesis</topic><topic>Neurotransmitter Agents - biosynthesis</topic><topic>Paraventricular Hypothalamic Nucleus - metabolism</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates: endocrinology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOORE, Joseph P</creatorcontrib><creatorcontrib>BURGER, Laura L</creatorcontrib><creatorcontrib>DALKIN, Alan C</creatorcontrib><creatorcontrib>WINTERS, Stephen J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOORE, Joseph P</au><au>BURGER, Laura L</au><au>DALKIN, Alan C</au><au>WINTERS, Stephen J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pituitary Adenylate Cyclase Activating Polypeptide Messenger RNA in the Paraventricular Nucleus and Anterior Pituitary During the Rat Estrous Cycle</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>73</volume><issue>3</issue><spage>491</spage><epage>499</epage><pages>491-499</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The neuropeptide pituitary adenylate cyclase activating polypeptide (ADCYAP 1, or PACAP) has been demonstrated to enhance
gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulate gonadotropin subunit gene expression in
cultures of anterior pituitary cells. In the present study, we used in situ hybridization and real-time polymerase chain reaction
to examine the expression of Pacap mRNA within the paraventricular nucleus (PVN) and anterior pituitary throughout the estrous cycle of the rat. Levels of luteinizing
hormone in serum and pituitary gonadotropin subunit mRNAs were evaluated and displayed cyclic fluctuations similar to those
reported previously. Pacap mRNA expression in the PVN and pituitary varied significantly during the estrous cycle, with the greatest changes occurring
on the day of proestrus. Pacap mRNA levels in the PVN declined significantly on the morning of diestrus. During proestrus, PVN Pacap mRNA levels significantly increased 3 h before the gonadotropin surge and then declined. Pituitary expression of Pacap mRNA also varied on the afternoon of proestrus with a moderate decline at the time of the gonadotropin surge and a significant
increase later in the evening. Expression of the mRNA species encoding the 288 amino acid form of follistatin increased significantly
following the rise in pituitary Pacap mRNA, at the termination of the secondary surge in follicle-stimulating hormone beta ( Fshb ) gene expression. These results suggest that PACAP is involved in events before and following the gonadotropin surge, perhaps
through increased gonadotroph sensitivity to GnRH and suppression of Fshb subunit expression through increased follistatin, as previously observed in vitro.
Abstract
In the rat, pituitary adenylate cyclase-activating polypeptide expression in the paraventricular nucleus and pituitary varies
during the estrous cycle and is correlated with gonadotropin synthesis and secretion</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15917345</pmid><doi>10.1095/biolreprod.105.041624</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 2005-09, Vol.73 (3), p.491-499 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Animals Biological and medical sciences Circadian Rhythm Estrous Cycle - metabolism Female Follicle Stimulating Hormone, beta Subunit - metabolism Follistatin - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation Gonadotropin-Releasing Hormone - physiology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Nerve Growth Factors - biosynthesis Neuropeptides - biosynthesis Neurotransmitter Agents - biosynthesis Paraventricular Hypothalamic Nucleus - metabolism Pituitary Adenylate Cyclase-Activating Polypeptide Pituitary Gland, Anterior - metabolism Rats Rats, Sprague-Dawley RNA, Messenger - metabolism Vertebrates: endocrinology Vertebrates: reproduction |
| title | Pituitary Adenylate Cyclase Activating Polypeptide Messenger RNA in the Paraventricular Nucleus and Anterior Pituitary During the Rat Estrous Cycle |
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