Purification of normal cellular prion protein from human platelets and the formation of a high molecular weight prion protein complex following platelet activation

A method for the extraction and purification of PrP C, in its native monomeric form, from outdated human platelet concentrates is described. Both calcium ionophore platelet activation and lysis in Triton X-100 were evaluated as methods for the extraction of soluble platelet PrP C in its monomeric fo...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-09, Vol.335 (1), p.48-56
Hauptverfasser:	Jones, Michael, Head, Mark W., Connolly, John G., Farquhar, Christine F., Hornsey, Valerie S., Pepper, Duncan S., MacGregor, Ian R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Blood Platelets - drug effects Blood Platelets - metabolism Blotting, Western Calcium - metabolism Calcium ionophore activation Cation exchange Chromatography, Affinity Chromatography, Ion Exchange Conformation change Copper - chemistry Copper - pharmacology Copper affinity Creutzfeldt-Jakob disease Detergent lysis Dimer Electrophoresis, Polyacrylamide Gel Human prion protein Humans Ionophores - pharmacology Molecular Weight Octoxynol - pharmacology Platelet Platelet Activation - drug effects Prions - chemistry Prions - isolation & purification Prions - metabolism Protein Structure, Quaternary Purification
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