The Drosophila gene CG9918 codes for a pyrokinin-1 receptor
The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and se...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-09, Vol.335 (1), p.14-19 |
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| creator | Cazzamali, Giuseppe Torp, Malene Hauser, Frank Williamson, Michael Grimmelikhuijzen, Cornelis J.P. |
| description | The database from the
Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25
Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the
Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC
50, 5
×
10
−8
M). The receptor was also activated by other
Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-γ, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by
Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a
Drosophila pyrokinin-1 receptor gene. |
| doi_str_mv | 10.1016/j.bbrc.2005.07.038 |
| format | Article |
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Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25
Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the
Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC
50, 5
×
10
−8
M). The receptor was also activated by other
Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-γ, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by
Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a
Drosophila pyrokinin-1 receptor gene.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.07.038</identifier><identifier>PMID: 16054112</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Capability gene ; CHO Cells ; Cricetinae ; Diapause ; DNA, Complementary - genetics ; Drosophila ; Drosophila - chemistry ; Drosophila - genetics ; Drosophila - metabolism ; Drosophila Proteins - chemistry ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Ecdysis ; Evolution ; Exons - genetics ; G protein-coupled receptor ; GPCR ; Honey bee ; Hugin gene ; Insect ; Introns - genetics ; Malaria ; Molecular Sequence Data ; Mosquito ; Neurohormone ; Neuromedin ; Neuropeptide ; Neuropeptides - metabolism ; Peptide hormone ; Phylogeny ; Pyrokinin ; Receptors, G-Protein-Coupled - chemistry ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Sequence Alignment ; Sex pheromone ; Transcription, Genetic - genetics</subject><ispartof>Biochemical and biophysical research communications, 2005-09, Vol.335 (1), p.14-19</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-8a169a1f0bfb5cd29e95cbc776e12390a5638da897308c5b43f4d4cd9c7cce633</citedby><cites>FETCH-LOGICAL-c500t-8a169a1f0bfb5cd29e95cbc776e12390a5638da897308c5b43f4d4cd9c7cce633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.07.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16054112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cazzamali, Giuseppe</creatorcontrib><creatorcontrib>Torp, Malene</creatorcontrib><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Williamson, Michael</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><title>The Drosophila gene CG9918 codes for a pyrokinin-1 receptor</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The database from the
Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25
Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the
Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC
50, 5
×
10
−8
M). The receptor was also activated by other
Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-γ, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by
Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a
Drosophila pyrokinin-1 receptor gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Capability gene</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Diapause</subject><subject>DNA, Complementary - genetics</subject><subject>Drosophila</subject><subject>Drosophila - chemistry</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - chemistry</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Ecdysis</subject><subject>Evolution</subject><subject>Exons - genetics</subject><subject>G protein-coupled receptor</subject><subject>GPCR</subject><subject>Honey bee</subject><subject>Hugin gene</subject><subject>Insect</subject><subject>Introns - genetics</subject><subject>Malaria</subject><subject>Molecular Sequence Data</subject><subject>Mosquito</subject><subject>Neurohormone</subject><subject>Neuromedin</subject><subject>Neuropeptide</subject><subject>Neuropeptides - metabolism</subject><subject>Peptide hormone</subject><subject>Phylogeny</subject><subject>Pyrokinin</subject><subject>Receptors, G-Protein-Coupled - chemistry</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Sequence Alignment</subject><subject>Sex pheromone</subject><subject>Transcription, Genetic - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD9PwzAQRy0EoqXwBRhQJraEu_xxbMGCChSkSixFYrMc50Jd2jjYLVK_PalaiQ2mW97vSfcYu0RIEJDfLJKq8iZJAYoEygQyccSGCBLiFCE_ZkMA4HEq8X3AzkJYACDmXJ6yAXIocsR0yG5nc4oevAuum9uljj6opWg8kRJFZFxNIWqcj3TUbb37tK1tY4w8GerWzp-zk0YvA10c7oi9PT3Oxs_x9HXyMr6fxqYAWMdCI5caG6iaqjB1KkkWpjJlyQnTTIIueCZqLWSZgTBFlWdNXuemlqY0hniWjdj13tt597WhsFYrGwwtl7oltwmKi1wIWeC_IJaZ5HkpezDdg6b_PHhqVOftSvutQlC7tmqhdm3Vrq2CUvVt-9HVwb6pVlT_Tg4xe-BuD1Af49uSV8FYag3Vtk-2VrWzf_l_AIwxiG8</recordid><startdate>20050916</startdate><enddate>20050916</enddate><creator>Cazzamali, Giuseppe</creator><creator>Torp, Malene</creator><creator>Hauser, Frank</creator><creator>Williamson, Michael</creator><creator>Grimmelikhuijzen, Cornelis J.P.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050916</creationdate><title>The Drosophila gene CG9918 codes for a pyrokinin-1 receptor</title><author>Cazzamali, Giuseppe ; Torp, Malene ; Hauser, Frank ; Williamson, Michael ; Grimmelikhuijzen, Cornelis J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-8a169a1f0bfb5cd29e95cbc776e12390a5638da897308c5b43f4d4cd9c7cce633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Capability gene</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Diapause</topic><topic>DNA, Complementary - genetics</topic><topic>Drosophila</topic><topic>Drosophila - chemistry</topic><topic>Drosophila - genetics</topic><topic>Drosophila - metabolism</topic><topic>Drosophila Proteins - chemistry</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Ecdysis</topic><topic>Evolution</topic><topic>Exons - genetics</topic><topic>G protein-coupled receptor</topic><topic>GPCR</topic><topic>Honey bee</topic><topic>Hugin gene</topic><topic>Insect</topic><topic>Introns - genetics</topic><topic>Malaria</topic><topic>Molecular Sequence Data</topic><topic>Mosquito</topic><topic>Neurohormone</topic><topic>Neuromedin</topic><topic>Neuropeptide</topic><topic>Neuropeptides - metabolism</topic><topic>Peptide hormone</topic><topic>Phylogeny</topic><topic>Pyrokinin</topic><topic>Receptors, G-Protein-Coupled - chemistry</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Sequence Alignment</topic><topic>Sex pheromone</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cazzamali, Giuseppe</creatorcontrib><creatorcontrib>Torp, Malene</creatorcontrib><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Williamson, Michael</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cazzamali, Giuseppe</au><au>Torp, Malene</au><au>Hauser, Frank</au><au>Williamson, Michael</au><au>Grimmelikhuijzen, Cornelis J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Drosophila gene CG9918 codes for a pyrokinin-1 receptor</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-09-16</date><risdate>2005</risdate><volume>335</volume><issue>1</issue><spage>14</spage><epage>19</epage><pages>14-19</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The database from the
Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25
Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the
Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC
50, 5
×
10
−8
M). The receptor was also activated by other
Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-γ, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by
Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a
Drosophila pyrokinin-1 receptor gene.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16054112</pmid><doi>10.1016/j.bbrc.2005.07.038</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Capability gene CHO Cells Cricetinae Diapause DNA, Complementary - genetics Drosophila Drosophila - chemistry Drosophila - genetics Drosophila - metabolism Drosophila Proteins - chemistry Drosophila Proteins - genetics Drosophila Proteins - metabolism Ecdysis Evolution Exons - genetics G protein-coupled receptor GPCR Honey bee Hugin gene Insect Introns - genetics Malaria Molecular Sequence Data Mosquito Neurohormone Neuromedin Neuropeptide Neuropeptides - metabolism Peptide hormone Phylogeny Pyrokinin Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Sequence Alignment Sex pheromone Transcription, Genetic - genetics |
| title | The Drosophila gene CG9918 codes for a pyrokinin-1 receptor |
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