The response of primary articular chondrocytes to micrometric surface topography and sulphated hyaluronic acid-based matrices
Understanding the response of chondrocytes to topographical cues and chemical patterns could provide invaluable information to advance the repair of chondral lesions. We studied the response of primary chondrocytes to nano- and micro-grooved surfaces, and sulphated hyaluronic acid (HyalS). Cells wer...
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| Veröffentlicht in: | Cell biology international 2005-08, Vol.29 (8), p.605-615 |
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| creator | Hamilton, D.W. Riehle, M.O. Rappuoli, R. Monaghan, W. Barbucci, R. Curtis, A.S.G. |
| description | Understanding the response of chondrocytes to topographical cues and chemical patterns could provide invaluable information to advance the repair of chondral lesions. We studied the response of primary chondrocytes to nano- and micro-grooved surfaces, and sulphated hyaluronic acid (HyalS). Cells were grown on grooves ranging from 80 nm to 9 μm in depth, and from 2 μm to 20 μm in width. Observations showed that the cells did not spread appreciably on any groove size, or alter morphology or F-actin organization, although cells showed accelerated movement on 750 nm deep grooves in comparison to flat surfaces. On chemical patterns, the cells migrated onto, and preferentially attached to, HyalS and showed a greater degree of spreading and F-actin re-arrangement. This study shows that 750 nm deep grooves and sulphated hyaluronic acid elicit responses from primary chondrocytes, and this could have implications for the future direction of cartilage reconstruction and orthopaedic treatments in general. |
| doi_str_mv | 10.1016/j.cellbi.2005.03.013 |
| format | Article |
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We studied the response of primary chondrocytes to nano- and micro-grooved surfaces, and sulphated hyaluronic acid (HyalS). Cells were grown on grooves ranging from 80 nm to 9 μm in depth, and from 2 μm to 20 μm in width. Observations showed that the cells did not spread appreciably on any groove size, or alter morphology or F-actin organization, although cells showed accelerated movement on 750 nm deep grooves in comparison to flat surfaces. On chemical patterns, the cells migrated onto, and preferentially attached to, HyalS and showed a greater degree of spreading and F-actin re-arrangement. This study shows that 750 nm deep grooves and sulphated hyaluronic acid elicit responses from primary chondrocytes, and this could have implications for the future direction of cartilage reconstruction and orthopaedic treatments in general.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1016/j.cellbi.2005.03.013</identifier><identifier>PMID: 15985378</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Actins - metabolism ; Animals ; Biocompatible Materials ; Cartilage, Articular - cytology ; Cell adhesion ; Cell Adhesion - physiology ; Cell Culture Techniques ; Cell migration ; Cell Movement - physiology ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Chondrocytes - ultrastructure ; Cytoskeleton - metabolism ; F-actin ; Hyaluronic Acid - analogs & derivatives ; Hyaluronic Acid - pharmacology ; Microscopy, Atomic Force ; Ovine chondrocytes ; Sheep ; Sulphated hyaluronic acid ; Surface Properties - drug effects ; Topography</subject><ispartof>Cell biology international, 2005-08, Vol.29 (8), p.605-615</ispartof><rights>2005 International Federation for Cell Biology</rights><rights>The Author(s) Journal compilation © 2005 International Federation for Cell Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5157-cbeb7a4d767ae957c61eda58abcea16f1c71e98f4a4a530238a18652599d5eee3</citedby><cites>FETCH-LOGICAL-c5157-cbeb7a4d767ae957c61eda58abcea16f1c71e98f4a4a530238a18652599d5eee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.cellbi.2005.03.013$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.cellbi.2005.03.013$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15985378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamilton, D.W.</creatorcontrib><creatorcontrib>Riehle, M.O.</creatorcontrib><creatorcontrib>Rappuoli, R.</creatorcontrib><creatorcontrib>Monaghan, W.</creatorcontrib><creatorcontrib>Barbucci, R.</creatorcontrib><creatorcontrib>Curtis, A.S.G.</creatorcontrib><title>The response of primary articular chondrocytes to micrometric surface topography and sulphated hyaluronic acid-based matrices</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Understanding the response of chondrocytes to topographical cues and chemical patterns could provide invaluable information to advance the repair of chondral lesions. We studied the response of primary chondrocytes to nano- and micro-grooved surfaces, and sulphated hyaluronic acid (HyalS). Cells were grown on grooves ranging from 80 nm to 9 μm in depth, and from 2 μm to 20 μm in width. Observations showed that the cells did not spread appreciably on any groove size, or alter morphology or F-actin organization, although cells showed accelerated movement on 750 nm deep grooves in comparison to flat surfaces. On chemical patterns, the cells migrated onto, and preferentially attached to, HyalS and showed a greater degree of spreading and F-actin re-arrangement. This study shows that 750 nm deep grooves and sulphated hyaluronic acid elicit responses from primary chondrocytes, and this could have implications for the future direction of cartilage reconstruction and orthopaedic treatments in general.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Cartilage, Articular - cytology</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Culture Techniques</subject><subject>Cell migration</subject><subject>Cell Movement - physiology</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - drug effects</subject><subject>Chondrocytes - ultrastructure</subject><subject>Cytoskeleton - metabolism</subject><subject>F-actin</subject><subject>Hyaluronic Acid - analogs & derivatives</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Microscopy, Atomic Force</subject><subject>Ovine chondrocytes</subject><subject>Sheep</subject><subject>Sulphated hyaluronic acid</subject><subject>Surface Properties - drug effects</subject><subject>Topography</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFr1TAUgIs43Jz-A5E--daa3DRp6oOgF90GY0M2GfgSTpNTm2vbdEmr3gf_uym9uDfZU8LJ9x3IlySvKMkpoeLtLtfYdbXNN4TwnLCcUPYkOaGk4plknD9d7oJnoqr4cfI8hB0hlBZSPEuOKa8kZ6U8Sf7ctph6DKMbAqauSUdve_D7FPxk9dyBT3XrBuOd3k8Y0smlvdXe9Th5q9Mw-wY0xvHovnsY2ygOJo67sYUJTdruoZu9GyIL2pqshhCnPSw2hhfJUQNdwJeH8zT5-vnT7fY8u7w-u9h-uMw0p7zMdI11CYUpRQlY8VILiga4hFojUNFQXVKsZFNAAZyRDZNApeAbXlWGIyI7Td6se0fv7mcMk-ptWPLBgG4OSshCElnRCBYrGL8YgsdGHXooStSSXe3Uml0t2RVhKmaP2uvD_rnu0TxIh84ReLcCv2yH-0ctVduPF1dUiDLK2SrbMOHvfzL4Hyq-llzdXZ2p8xt2x26-UPUt8u9XHmPSnxa9CtrioNFYj3pSxtn_f-cvAgC65Q</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Hamilton, D.W.</creator><creator>Riehle, M.O.</creator><creator>Rappuoli, R.</creator><creator>Monaghan, W.</creator><creator>Barbucci, R.</creator><creator>Curtis, A.S.G.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>The response of primary articular chondrocytes to micrometric surface topography and sulphated hyaluronic acid-based matrices</title><author>Hamilton, D.W. ; Riehle, M.O. ; Rappuoli, R. ; Monaghan, W. ; Barbucci, R. ; Curtis, A.S.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5157-cbeb7a4d767ae957c61eda58abcea16f1c71e98f4a4a530238a18652599d5eee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Cartilage, Articular - cytology</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Culture Techniques</topic><topic>Cell migration</topic><topic>Cell Movement - physiology</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - drug effects</topic><topic>Chondrocytes - ultrastructure</topic><topic>Cytoskeleton - metabolism</topic><topic>F-actin</topic><topic>Hyaluronic Acid - analogs & derivatives</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Microscopy, Atomic Force</topic><topic>Ovine chondrocytes</topic><topic>Sheep</topic><topic>Sulphated hyaluronic acid</topic><topic>Surface Properties - drug effects</topic><topic>Topography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamilton, D.W.</creatorcontrib><creatorcontrib>Riehle, M.O.</creatorcontrib><creatorcontrib>Rappuoli, R.</creatorcontrib><creatorcontrib>Monaghan, W.</creatorcontrib><creatorcontrib>Barbucci, R.</creatorcontrib><creatorcontrib>Curtis, A.S.G.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamilton, D.W.</au><au>Riehle, M.O.</au><au>Rappuoli, R.</au><au>Monaghan, W.</au><au>Barbucci, R.</au><au>Curtis, A.S.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The response of primary articular chondrocytes to micrometric surface topography and sulphated hyaluronic acid-based matrices</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2005-08</date><risdate>2005</risdate><volume>29</volume><issue>8</issue><spage>605</spage><epage>615</epage><pages>605-615</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Understanding the response of chondrocytes to topographical cues and chemical patterns could provide invaluable information to advance the repair of chondral lesions. We studied the response of primary chondrocytes to nano- and micro-grooved surfaces, and sulphated hyaluronic acid (HyalS). Cells were grown on grooves ranging from 80 nm to 9 μm in depth, and from 2 μm to 20 μm in width. Observations showed that the cells did not spread appreciably on any groove size, or alter morphology or F-actin organization, although cells showed accelerated movement on 750 nm deep grooves in comparison to flat surfaces. On chemical patterns, the cells migrated onto, and preferentially attached to, HyalS and showed a greater degree of spreading and F-actin re-arrangement. This study shows that 750 nm deep grooves and sulphated hyaluronic acid elicit responses from primary chondrocytes, and this could have implications for the future direction of cartilage reconstruction and orthopaedic treatments in general.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>15985378</pmid><doi>10.1016/j.cellbi.2005.03.013</doi><tpages>11</tpages></addata></record> |
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| subjects | Actins - metabolism Animals Biocompatible Materials Cartilage, Articular - cytology Cell adhesion Cell Adhesion - physiology Cell Culture Techniques Cell migration Cell Movement - physiology Chondrocytes - cytology Chondrocytes - drug effects Chondrocytes - ultrastructure Cytoskeleton - metabolism F-actin Hyaluronic Acid - analogs & derivatives Hyaluronic Acid - pharmacology Microscopy, Atomic Force Ovine chondrocytes Sheep Sulphated hyaluronic acid Surface Properties - drug effects Topography |
| title | The response of primary articular chondrocytes to micrometric surface topography and sulphated hyaluronic acid-based matrices |
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