Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo ( Bubalus bubalis)
Livestock are commonly infected with protozoan parasites of the genera Cryptosporidium and Giardia, and some of the species and genotypes found in these animals have zoonotic significance. We characterized isolates of both parasites recovered from the Italian water buffalo ( Bubalus bubalis), an eco...
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| Veröffentlicht in: | Veterinary parasitology 2007-11, Vol.150 (1), p.146-149 |
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| creator | Cacciò, Simone M. Rinaldi, Laura Cringoli, Giuseppe Condoleo, Renato Pozio, Edoardo |
| description | Livestock are commonly infected with protozoan parasites of the genera
Cryptosporidium and
Giardia, and some of the species and genotypes found in these animals have zoonotic significance. We characterized isolates of both parasites recovered from the Italian water buffalo (
Bubalus bubalis), an economically important species whose milk is used for the production of “buffalo mozzarella” fresh cheese. Molecular analysis of the
Cryptosporidium small subunit ribosomal DNA gene and of the
Giardia β-giardin gene shows the presence of both zoonotic parasites (
Cryptosporidium parvum and
Giardia duodenalis assemblage A) and host-specific parasites (
G. duodenalis assemblage E), suggesting that water buffaloes can contribute to environmental contamination with oocysts and cysts potentially infectious to humans if their faeces are improperly disposed of. On the other hand, mozzarella cheese is probably a safe product, given that its production involves the treatment of cheese curd at 85–95
°C, which is likely to kill or inactivate the parasites. |
| doi_str_mv | 10.1016/j.vetpar.2007.09.013 |
| format | Article |
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Cryptosporidium and
Giardia, and some of the species and genotypes found in these animals have zoonotic significance. We characterized isolates of both parasites recovered from the Italian water buffalo (
Bubalus bubalis), an economically important species whose milk is used for the production of “buffalo mozzarella” fresh cheese. Molecular analysis of the
Cryptosporidium small subunit ribosomal DNA gene and of the
Giardia β-giardin gene shows the presence of both zoonotic parasites (
Cryptosporidium parvum and
Giardia duodenalis assemblage A) and host-specific parasites (
G. duodenalis assemblage E), suggesting that water buffaloes can contribute to environmental contamination with oocysts and cysts potentially infectious to humans if their faeces are improperly disposed of. On the other hand, mozzarella cheese is probably a safe product, given that its production involves the treatment of cheese curd at 85–95
°C, which is likely to kill or inactivate the parasites.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2007.09.013</identifier><identifier>PMID: 17951009</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bubalus bubalis ; buffaloes ; Buffaloes - parasitology ; cryptosporidiosis ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium parvum ; Cryptosporidium parvum - genetics ; Cryptosporidium parvum - isolation & purification ; cysts (developmental stages) ; DNA, Protozoan - classification ; DNA, Protozoan - genetics ; DNA, Protozoan - isolation & purification ; feces ; genes ; genotype ; Giardia ; Giardia - classification ; Giardia - genetics ; Giardia - isolation & purification ; Giardia duodenalis ; Giardia lamblia ; giardiasis ; Giardiasis - parasitology ; Giardiasis - veterinary ; host strains ; Italy ; Milk ; molecular epidemiology ; Molecular typing ; Mozzarella cheese ; oocysts ; pathogen identification ; ribosomal DNA ; sequence analysis ; Water buffalo ; zoonoses</subject><ispartof>Veterinary parasitology, 2007-11, Vol.150 (1), p.146-149</ispartof><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-34892fac1b59fa50ddbf61a817d0cebbc3b8a4655b5224989ba0699957aa6f383</citedby><cites>FETCH-LOGICAL-c481t-34892fac1b59fa50ddbf61a817d0cebbc3b8a4655b5224989ba0699957aa6f383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401707004980$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17951009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cacciò, Simone M.</creatorcontrib><creatorcontrib>Rinaldi, Laura</creatorcontrib><creatorcontrib>Cringoli, Giuseppe</creatorcontrib><creatorcontrib>Condoleo, Renato</creatorcontrib><creatorcontrib>Pozio, Edoardo</creatorcontrib><title>Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo ( Bubalus bubalis)</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Livestock are commonly infected with protozoan parasites of the genera
Cryptosporidium and
Giardia, and some of the species and genotypes found in these animals have zoonotic significance. We characterized isolates of both parasites recovered from the Italian water buffalo (
Bubalus bubalis), an economically important species whose milk is used for the production of “buffalo mozzarella” fresh cheese. Molecular analysis of the
Cryptosporidium small subunit ribosomal DNA gene and of the
Giardia β-giardin gene shows the presence of both zoonotic parasites (
Cryptosporidium parvum and
Giardia duodenalis assemblage A) and host-specific parasites (
G. duodenalis assemblage E), suggesting that water buffaloes can contribute to environmental contamination with oocysts and cysts potentially infectious to humans if their faeces are improperly disposed of. On the other hand, mozzarella cheese is probably a safe product, given that its production involves the treatment of cheese curd at 85–95
°C, which is likely to kill or inactivate the parasites.</description><subject>Animals</subject><subject>Bubalus bubalis</subject><subject>buffaloes</subject><subject>Buffaloes - parasitology</subject><subject>cryptosporidiosis</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium parvum</subject><subject>Cryptosporidium parvum - genetics</subject><subject>Cryptosporidium parvum - isolation & purification</subject><subject>cysts (developmental stages)</subject><subject>DNA, Protozoan - classification</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Protozoan - isolation & purification</subject><subject>feces</subject><subject>genes</subject><subject>genotype</subject><subject>Giardia</subject><subject>Giardia - classification</subject><subject>Giardia - genetics</subject><subject>Giardia - isolation & purification</subject><subject>Giardia duodenalis</subject><subject>Giardia lamblia</subject><subject>giardiasis</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - veterinary</subject><subject>host strains</subject><subject>Italy</subject><subject>Milk</subject><subject>molecular epidemiology</subject><subject>Molecular typing</subject><subject>Mozzarella cheese</subject><subject>oocysts</subject><subject>pathogen identification</subject><subject>ribosomal DNA</subject><subject>sequence analysis</subject><subject>Water buffalo</subject><subject>zoonoses</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhiMEYsvCP0DgE4JDwzixE_uCBBUsKy3iAHu2xl_gKo2DnRT13-MqlbjBaWTP846tearqOYWaAu3e7uujmydMdQPQ1yBroO2DakNF324bzuFhtYEW2JYB7a-qJznvAYBB1z-urmgvOQWQm-r0JQ7OLAMmEqwb5-CDwTnEkURPduk0zTFPMQUblgMprx1LwdGSm4DJBiR2iSWGQ8gkjGT-6cjtXE44kt84u0T04j0OkbwmHxaNw5LLjT7jb55Wj0onu2eXel3df_r4ffd5e_f15nb3_m5rmKDztmVCNh4N1Vx65GCt9h1FQXsLxmltWi2QdZxr3jRMCqkROikl7xE734r2unq1zp1S_LW4PKtDyMYNA44uLll1gvVSdvBfkBao4S0tIFtBk2LOyXk1pXDAdFIU1NmN2qvVjTq7USBVcVNiLy7zF31w9m_oIqMAL1fAY1T4I4Ws7r81JQogWNMKXoh3K-HKwo7BJZVNcKNxNiRnZmVj-Pcf_gBjkqzJ</recordid><startdate>20071130</startdate><enddate>20071130</enddate><creator>Cacciò, Simone M.</creator><creator>Rinaldi, Laura</creator><creator>Cringoli, Giuseppe</creator><creator>Condoleo, Renato</creator><creator>Pozio, Edoardo</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20071130</creationdate><title>Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo ( Bubalus bubalis)</title><author>Cacciò, Simone M. ; Rinaldi, Laura ; Cringoli, Giuseppe ; Condoleo, Renato ; Pozio, Edoardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-34892fac1b59fa50ddbf61a817d0cebbc3b8a4655b5224989ba0699957aa6f383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bubalus bubalis</topic><topic>buffaloes</topic><topic>Buffaloes - parasitology</topic><topic>cryptosporidiosis</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium parvum</topic><topic>Cryptosporidium parvum - genetics</topic><topic>Cryptosporidium parvum - isolation & purification</topic><topic>cysts (developmental stages)</topic><topic>DNA, Protozoan - classification</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Protozoan - isolation & purification</topic><topic>feces</topic><topic>genes</topic><topic>genotype</topic><topic>Giardia</topic><topic>Giardia - classification</topic><topic>Giardia - genetics</topic><topic>Giardia - isolation & purification</topic><topic>Giardia duodenalis</topic><topic>Giardia lamblia</topic><topic>giardiasis</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - veterinary</topic><topic>host strains</topic><topic>Italy</topic><topic>Milk</topic><topic>molecular epidemiology</topic><topic>Molecular typing</topic><topic>Mozzarella cheese</topic><topic>oocysts</topic><topic>pathogen identification</topic><topic>ribosomal DNA</topic><topic>sequence analysis</topic><topic>Water buffalo</topic><topic>zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cacciò, Simone M.</creatorcontrib><creatorcontrib>Rinaldi, Laura</creatorcontrib><creatorcontrib>Cringoli, Giuseppe</creatorcontrib><creatorcontrib>Condoleo, Renato</creatorcontrib><creatorcontrib>Pozio, Edoardo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cacciò, Simone M.</au><au>Rinaldi, Laura</au><au>Cringoli, Giuseppe</au><au>Condoleo, Renato</au><au>Pozio, Edoardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo ( Bubalus bubalis)</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2007-11-30</date><risdate>2007</risdate><volume>150</volume><issue>1</issue><spage>146</spage><epage>149</epage><pages>146-149</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Livestock are commonly infected with protozoan parasites of the genera
Cryptosporidium and
Giardia, and some of the species and genotypes found in these animals have zoonotic significance. We characterized isolates of both parasites recovered from the Italian water buffalo (
Bubalus bubalis), an economically important species whose milk is used for the production of “buffalo mozzarella” fresh cheese. Molecular analysis of the
Cryptosporidium small subunit ribosomal DNA gene and of the
Giardia β-giardin gene shows the presence of both zoonotic parasites (
Cryptosporidium parvum and
Giardia duodenalis assemblage A) and host-specific parasites (
G. duodenalis assemblage E), suggesting that water buffaloes can contribute to environmental contamination with oocysts and cysts potentially infectious to humans if their faeces are improperly disposed of. On the other hand, mozzarella cheese is probably a safe product, given that its production involves the treatment of cheese curd at 85–95
°C, which is likely to kill or inactivate the parasites.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17951009</pmid><doi>10.1016/j.vetpar.2007.09.013</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary parasitology, 2007-11, Vol.150 (1), p.146-149 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68479960 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bubalus bubalis buffaloes Buffaloes - parasitology cryptosporidiosis Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium parvum Cryptosporidium parvum - genetics Cryptosporidium parvum - isolation & purification cysts (developmental stages) DNA, Protozoan - classification DNA, Protozoan - genetics DNA, Protozoan - isolation & purification feces genes genotype Giardia Giardia - classification Giardia - genetics Giardia - isolation & purification Giardia duodenalis Giardia lamblia giardiasis Giardiasis - parasitology Giardiasis - veterinary host strains Italy Milk molecular epidemiology Molecular typing Mozzarella cheese oocysts pathogen identification ribosomal DNA sequence analysis Water buffalo zoonoses |
| title | Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo ( Bubalus bubalis) |
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