Cloning, ontogenesis, and localization of an atypical uncoupling protein 4 in Xenopus laevis
1 Department of Cell Physiology and Metabolism, University Medical Center 1, Geneva 2 Division of Neuropsychiatry, Belle-Idée, Geneva University Hospital, Chene-Bourg, Switzerland Uncoupling protein 1 (UCP1) is the first UCP described. It belongs to the family of mitochondrial carrier proteins and i...
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| creator | Keller, Patrick A Lehr, Lorenz Giacobino, Jean-Paul Charnay, Yves Assimacopoulos-Jeannet, Francoise Giovannini, Natalia |
| description | 1 Department of Cell Physiology and Metabolism, University Medical Center 1, Geneva
2 Division of Neuropsychiatry, Belle-Idée, Geneva University Hospital, Chene-Bourg, Switzerland
Uncoupling protein 1 (UCP1) is the first UCP described. It belongs to the family of mitochondrial carrier proteins and is expressed mainly in brown adipose tissue. Recently, the family of the UCPs has rapidly been growing due to the successive cloning of UCP2, UCP3, UCP4, and UCP5, also called brain mitochondrial carrier protein 1. Phylogenetic studies suggest that UCP1/UCP2/UCP3 on one hand and UCP4/UCP5 on the other hand belong to separate subfamilies. In this study, we report the cloning from a frog Xenopus laevis (Xl) oocyte cDNA library of a novel UCP that was shown, by sequence homology, to belong to the family of ancestral UCP4. This cloning provides a milestone in the gap between Drosophila melanogaster or Caenorhabditis elegans on one hand and mammalian UCP4 on the other. Xl UCP4 is already expressed in the oocyte, being the first UCP described in germ cell lineage. During development, it segregates in the neural cord, and, in the adult, in situ hybridization shows its expression in the neurons and also in the choroid plexus of the brain. By RT-PCR analysis, it was found that Xl UCP4 is present in all the subdivisions of the brain and also that it differs from mammalian UCP4 by a very high relative level of expression in peripheral tissues such as the liver and kidney. The peripheral tissue distribution of Xl UCP4 reinforces the hypothesis that UCP4 might be the ancestral UCP from which other UCPs diverged from.
cDNA; cloning |
| doi_str_mv | 10.1152/physiolgenomics.00012.2005 |
| format | Article |
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2 Division of Neuropsychiatry, Belle-Idée, Geneva University Hospital, Chene-Bourg, Switzerland
Uncoupling protein 1 (UCP1) is the first UCP described. It belongs to the family of mitochondrial carrier proteins and is expressed mainly in brown adipose tissue. Recently, the family of the UCPs has rapidly been growing due to the successive cloning of UCP2, UCP3, UCP4, and UCP5, also called brain mitochondrial carrier protein 1. Phylogenetic studies suggest that UCP1/UCP2/UCP3 on one hand and UCP4/UCP5 on the other hand belong to separate subfamilies. In this study, we report the cloning from a frog Xenopus laevis (Xl) oocyte cDNA library of a novel UCP that was shown, by sequence homology, to belong to the family of ancestral UCP4. This cloning provides a milestone in the gap between Drosophila melanogaster or Caenorhabditis elegans on one hand and mammalian UCP4 on the other. Xl UCP4 is already expressed in the oocyte, being the first UCP described in germ cell lineage. During development, it segregates in the neural cord, and, in the adult, in situ hybridization shows its expression in the neurons and also in the choroid plexus of the brain. By RT-PCR analysis, it was found that Xl UCP4 is present in all the subdivisions of the brain and also that it differs from mammalian UCP4 by a very high relative level of expression in peripheral tissues such as the liver and kidney. The peripheral tissue distribution of Xl UCP4 reinforces the hypothesis that UCP4 might be the ancestral UCP from which other UCPs diverged from.
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2 Division of Neuropsychiatry, Belle-Idée, Geneva University Hospital, Chene-Bourg, Switzerland
Uncoupling protein 1 (UCP1) is the first UCP described. It belongs to the family of mitochondrial carrier proteins and is expressed mainly in brown adipose tissue. Recently, the family of the UCPs has rapidly been growing due to the successive cloning of UCP2, UCP3, UCP4, and UCP5, also called brain mitochondrial carrier protein 1. Phylogenetic studies suggest that UCP1/UCP2/UCP3 on one hand and UCP4/UCP5 on the other hand belong to separate subfamilies. In this study, we report the cloning from a frog Xenopus laevis (Xl) oocyte cDNA library of a novel UCP that was shown, by sequence homology, to belong to the family of ancestral UCP4. This cloning provides a milestone in the gap between Drosophila melanogaster or Caenorhabditis elegans on one hand and mammalian UCP4 on the other. Xl UCP4 is already expressed in the oocyte, being the first UCP described in germ cell lineage. During development, it segregates in the neural cord, and, in the adult, in situ hybridization shows its expression in the neurons and also in the choroid plexus of the brain. By RT-PCR analysis, it was found that Xl UCP4 is present in all the subdivisions of the brain and also that it differs from mammalian UCP4 by a very high relative level of expression in peripheral tissues such as the liver and kidney. The peripheral tissue distribution of Xl UCP4 reinforces the hypothesis that UCP4 might be the ancestral UCP from which other UCPs diverged from.
cDNA; cloning</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Anura</subject><subject>Blotting, Northern</subject><subject>Brain - metabolism</subject><subject>Caenorhabditis elegans</subject><subject>Carrier Proteins - chemistry</subject><subject>Cell Lineage</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - metabolism</subject><subject>Drosophila melanogaster</subject><subject>Evolution, Molecular</subject><subject>Expressed Sequence Tags</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Library</subject><subject>Germ Cells - metabolism</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Kidney - metabolism</subject><subject>Liver - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Membrane Transport Proteins - physiology</subject><subject>Mitochondrial Uncoupling Proteins</subject><subject>Models, Anatomic</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tissue Distribution</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis - metabolism</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - physiology</subject><issn>1094-8341</issn><issn>1531-2267</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYNYbK1-BQk--NTZ5ubPJOOLlMVqoeBLBR-EkM0ku5FsMk5m1PXTm7pbCgviSxIuv3PuIQeh10AWAIJeDptdCTmuXcrbYMuCEAJ0QQkRT9AZCAYNpa18Wt-k441iHE7R81K-VYxLJZ6hUxAdcKHIGfq6jDmFtL7AOU25WroSygU2qccxWxPDbzOFnHD2dYbNtBtCneI52TwPsQrxMObJhYQ5rseXmmmYC47G_QjlBTrxJhb38nCfo8_X7--WH5vbTx9ulle3jeVcTY0g0DMJ0DEGK-dlK8Wqt8Q66sFy6lQrOHSyU5YSYwUVxtm2dc575TmFFTtHb_a-Ncv32ZVJb0OxLkaTXJ6LbhWXUgr4LwhdJxUBVsG3e9COuZTReT2MYWvGnQai70vQRyXovyXo-xKq-NVhy7zauv5Revj1CrzbA5uw3vwMo3twy-udvp5jvHO_puMNlGqmGev00PvqwP7tcBztQcn-ADuHtEg</recordid><startdate>20050811</startdate><enddate>20050811</enddate><creator>Keller, Patrick A</creator><creator>Lehr, Lorenz</creator><creator>Giacobino, Jean-Paul</creator><creator>Charnay, Yves</creator><creator>Assimacopoulos-Jeannet, Francoise</creator><creator>Giovannini, Natalia</creator><general>Am Physiological Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050811</creationdate><title>Cloning, ontogenesis, and localization of an atypical uncoupling protein 4 in Xenopus laevis</title><author>Keller, Patrick A ; Lehr, Lorenz ; Giacobino, Jean-Paul ; Charnay, Yves ; Assimacopoulos-Jeannet, Francoise ; Giovannini, Natalia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-501d37119331bef7675bdc0ce2f1c42e865419798c20ac525aec66eeff8f421b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Anura</topic><topic>Blotting, Northern</topic><topic>Brain - metabolism</topic><topic>Caenorhabditis elegans</topic><topic>Carrier Proteins - chemistry</topic><topic>Cell Lineage</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - metabolism</topic><topic>Drosophila melanogaster</topic><topic>Evolution, Molecular</topic><topic>Expressed Sequence Tags</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Library</topic><topic>Germ Cells - metabolism</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Kidney - metabolism</topic><topic>Liver - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Membrane Transport Proteins - physiology</topic><topic>Mitochondrial Uncoupling Proteins</topic><topic>Models, Anatomic</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tissue Distribution</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - metabolism</topic><topic>Xenopus Proteins - genetics</topic><topic>Xenopus Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keller, Patrick A</creatorcontrib><creatorcontrib>Lehr, Lorenz</creatorcontrib><creatorcontrib>Giacobino, Jean-Paul</creatorcontrib><creatorcontrib>Charnay, Yves</creatorcontrib><creatorcontrib>Assimacopoulos-Jeannet, Francoise</creatorcontrib><creatorcontrib>Giovannini, Natalia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keller, Patrick A</au><au>Lehr, Lorenz</au><au>Giacobino, Jean-Paul</au><au>Charnay, Yves</au><au>Assimacopoulos-Jeannet, Francoise</au><au>Giovannini, Natalia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, ontogenesis, and localization of an atypical uncoupling protein 4 in Xenopus laevis</atitle><jtitle>Physiological genomics</jtitle><addtitle>Physiol Genomics</addtitle><date>2005-08-11</date><risdate>2005</risdate><volume>22</volume><issue>3</issue><spage>339</spage><epage>345</epage><pages>339-345</pages><issn>1094-8341</issn><eissn>1531-2267</eissn><abstract>1 Department of Cell Physiology and Metabolism, University Medical Center 1, Geneva
2 Division of Neuropsychiatry, Belle-Idée, Geneva University Hospital, Chene-Bourg, Switzerland
Uncoupling protein 1 (UCP1) is the first UCP described. It belongs to the family of mitochondrial carrier proteins and is expressed mainly in brown adipose tissue. Recently, the family of the UCPs has rapidly been growing due to the successive cloning of UCP2, UCP3, UCP4, and UCP5, also called brain mitochondrial carrier protein 1. Phylogenetic studies suggest that UCP1/UCP2/UCP3 on one hand and UCP4/UCP5 on the other hand belong to separate subfamilies. In this study, we report the cloning from a frog Xenopus laevis (Xl) oocyte cDNA library of a novel UCP that was shown, by sequence homology, to belong to the family of ancestral UCP4. This cloning provides a milestone in the gap between Drosophila melanogaster or Caenorhabditis elegans on one hand and mammalian UCP4 on the other. Xl UCP4 is already expressed in the oocyte, being the first UCP described in germ cell lineage. During development, it segregates in the neural cord, and, in the adult, in situ hybridization shows its expression in the neurons and also in the choroid plexus of the brain. By RT-PCR analysis, it was found that Xl UCP4 is present in all the subdivisions of the brain and also that it differs from mammalian UCP4 by a very high relative level of expression in peripheral tissues such as the liver and kidney. The peripheral tissue distribution of Xl UCP4 reinforces the hypothesis that UCP4 might be the ancestral UCP from which other UCPs diverged from.
cDNA; cloning</abstract><cop>United States</cop><pub>Am Physiological Soc</pub><pmid>15914580</pmid><doi>10.1152/physiolgenomics.00012.2005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Physiological genomics, 2005-08, Vol.22 (3), p.339-345 |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Animals Anura Blotting, Northern Brain - metabolism Caenorhabditis elegans Carrier Proteins - chemistry Cell Lineage Cloning, Molecular DNA, Complementary - metabolism Drosophila melanogaster Evolution, Molecular Expressed Sequence Tags Gene Expression Regulation Gene Expression Regulation, Developmental Gene Library Germ Cells - metabolism Humans In Situ Hybridization Kidney - metabolism Liver - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Membrane Transport Proteins - physiology Mitochondrial Uncoupling Proteins Models, Anatomic Models, Biological Molecular Sequence Data Phylogeny Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Homology, Amino Acid Tissue Distribution Xenopus laevis Xenopus laevis - metabolism Xenopus Proteins - genetics Xenopus Proteins - physiology |
| title | Cloning, ontogenesis, and localization of an atypical uncoupling protein 4 in Xenopus laevis |
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