Peptide Nanotube-Modified Electrodes for Enzyme−Biosensor Applications
The fabrication and notably improved performance of composite electrodes based on modified self-assembled diphenylalanine peptide nanotubes is described. Peptide nanotubes were attached to gold electrodes, and we studied the resulting electrochemical behavior using cyclic voltammetry and chronoamper...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2005-08, Vol.77 (16), p.5155-5159 |
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| creator | Yemini, Miri Reches, Meital Gazit, Ehud Rishpon, Judith |
| description | The fabrication and notably improved performance of composite electrodes based on modified self-assembled diphenylalanine peptide nanotubes is described. Peptide nanotubes were attached to gold electrodes, and we studied the resulting electrochemical behavior using cyclic voltammetry and chronoamperometry. The peptide nanotube-based electrodes demonstrated a direct and unmediated response to hydrogen peroxide and NADH at a potential of +0.4 V (vs SCE). This biosensor enables a sensitive determination of glucose by monitoring the hydrogen peroxide produced by an enzymatic reaction between the glucose oxidase attached to the peptide nanotubes and glucose. In addition, the marked electrocatalytic activity toward NADH enabled a sensitive detection of ethanol using ethanol dehydrogenase and NAD+. The peptide nanotube-based amperometric biosensor provides a potential new tool for sensitive biosensors and biomolecular diagnostics. |
| doi_str_mv | 10.1021/ac050414g |
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Peptide nanotubes were attached to gold electrodes, and we studied the resulting electrochemical behavior using cyclic voltammetry and chronoamperometry. The peptide nanotube-based electrodes demonstrated a direct and unmediated response to hydrogen peroxide and NADH at a potential of +0.4 V (vs SCE). This biosensor enables a sensitive determination of glucose by monitoring the hydrogen peroxide produced by an enzymatic reaction between the glucose oxidase attached to the peptide nanotubes and glucose. In addition, the marked electrocatalytic activity toward NADH enabled a sensitive detection of ethanol using ethanol dehydrogenase and NAD+. The peptide nanotube-based amperometric biosensor provides a potential new tool for sensitive biosensors and biomolecular diagnostics.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac050414g</identifier><identifier>PMID: 16097753</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Chemistry ; Electrochemical methods ; Electrodes ; Enzymes ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Glucose - analysis ; Glucose - metabolism ; Glucose Oxidase - metabolism ; Gold ; Hydrogen Peroxide - metabolism ; Methods. Procedures. 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Chem</addtitle><description>The fabrication and notably improved performance of composite electrodes based on modified self-assembled diphenylalanine peptide nanotubes is described. Peptide nanotubes were attached to gold electrodes, and we studied the resulting electrochemical behavior using cyclic voltammetry and chronoamperometry. The peptide nanotube-based electrodes demonstrated a direct and unmediated response to hydrogen peroxide and NADH at a potential of +0.4 V (vs SCE). This biosensor enables a sensitive determination of glucose by monitoring the hydrogen peroxide produced by an enzymatic reaction between the glucose oxidase attached to the peptide nanotubes and glucose. In addition, the marked electrocatalytic activity toward NADH enabled a sensitive detection of ethanol using ethanol dehydrogenase and NAD+. 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Technologies</subject><subject>Microscopy, Electron, Scanning</subject><subject>NADP - metabolism</subject><subject>Nanotubes</subject><subject>Nanotubes, Peptide - chemistry</subject><subject>Nanotubes, Peptide - ultrastructure</subject><subject>Peptides</subject><subject>Sensitivity and Specificity</subject><subject>Various methods and equipments</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c2KFDEQAOAgijuuHnwBGQQFD62VpPPTx3UYd5VVB3bFY0jS1ZK1p9Mm3eD6BJ59RJ_ELDPsgB48FaQ-KvVDyGMKLykw-sp6EFDT-ssdsqCCQSW1ZnfJAgB4xRTAEXmQ8xUApUDlfXJEJTRKCb4gZxscp9Di8oMd4jQ7rN7HNnQB2-W6Rz-l2GJedjEt18OP6y3-_vnrdYgZh1yeTsaxD95OIQ75IbnX2T7jo308Jp_erC9XZ9X5x9O3q5PzytZKTpUELrnXXApmW95RYYEzZxvnnALltOMaOW0FcgaqEVSXoHhjeUlbx2p-TJ7v6o4pfpsxT2Ybsse-twPGORupyz-qYf-FtKkZsAYKfPoXvIpzGsoQhlGltRJ1U9CLHfIp5pywM2MKW5uuDQVzcwRze4Rin-wLzm6L7UHut17Asz2w2du-S3bwIR-cAgaS33RW7VzIE36_zdv01UjFlTCXmwvz7lRvLlaSms-HutbnwxD_NvgHLKqoFg</recordid><startdate>20050815</startdate><enddate>20050815</enddate><creator>Yemini, Miri</creator><creator>Reches, Meital</creator><creator>Gazit, Ehud</creator><creator>Rishpon, Judith</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050815</creationdate><title>Peptide Nanotube-Modified Electrodes for Enzyme−Biosensor Applications</title><author>Yemini, Miri ; Reches, Meital ; Gazit, Ehud ; Rishpon, Judith</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-60363c83652ad3f15a032ba9bbb707b8b38e31d5e32079518207739a3b70ab243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Electrochemical methods</topic><topic>Electrodes</topic><topic>Enzymes</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - analysis</topic><topic>Glucose - metabolism</topic><topic>Glucose Oxidase - metabolism</topic><topic>Gold</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Methods. Procedures. 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Chem</addtitle><date>2005-08-15</date><risdate>2005</risdate><volume>77</volume><issue>16</issue><spage>5155</spage><epage>5159</epage><pages>5155-5159</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The fabrication and notably improved performance of composite electrodes based on modified self-assembled diphenylalanine peptide nanotubes is described. Peptide nanotubes were attached to gold electrodes, and we studied the resulting electrochemical behavior using cyclic voltammetry and chronoamperometry. The peptide nanotube-based electrodes demonstrated a direct and unmediated response to hydrogen peroxide and NADH at a potential of +0.4 V (vs SCE). This biosensor enables a sensitive determination of glucose by monitoring the hydrogen peroxide produced by an enzymatic reaction between the glucose oxidase attached to the peptide nanotubes and glucose. 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| subjects | Analytical chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Chemistry Electrochemical methods Electrodes Enzymes Exact sciences and technology Fundamental and applied biological sciences. Psychology Glucose - analysis Glucose - metabolism Glucose Oxidase - metabolism Gold Hydrogen Peroxide - metabolism Methods. Procedures. Technologies Microscopy, Electron, Scanning NADP - metabolism Nanotubes Nanotubes, Peptide - chemistry Nanotubes, Peptide - ultrastructure Peptides Sensitivity and Specificity Various methods and equipments |
| title | Peptide Nanotube-Modified Electrodes for Enzyme−Biosensor Applications |
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