Differential Stripping: Determination of the Amount of Topically Applied Substances Penetrated into the Hair Follicles
The determination of penetration pathways of topically applied substances into the skin is the subject of several investigations. Recently, follicular penetration has become a major focus of interest. To date, a direct, non-invasive quantification of the amount of topically applied substance penetra...
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| Veröffentlicht in: | Journal of general internal medicine : JGIM 2005-08, Vol.125 (2), p.264-269 |
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| container_title | Journal of general internal medicine : JGIM |
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| creator | Teichmann, Alexa Jacobi, Ute Ossadnik, Michael Richter, Heike Koch, Stefan Sterry, Wolfram Lademann, Jürgen |
| description | The determination of penetration pathways of topically applied substances into the skin is the subject of several investigations. Recently, follicular penetration has become a major focus of interest. To date, a direct, non-invasive quantification of the amount of topically applied substance penetrated into the follicles had not been possible. The development of such a method was the aim of this study. Therefore, the advantages of both stripping techniques, tape stripping and cyanoacrylate skin surface biopsy, were combined and evaluated. Tape stripping was used to remove the part of the stratum corneum that contained the topically applied dye. Subsequently, the follicular contents were ripped off by cyanoacrylate skin surface biopsy. The combined method termed “differential stripping” was evaluated in vitro and in vivo, and the amount of topically applied fluorescent dye penetrated into the hair follicles was quantified after different penetration times. After 30 min, 5% of the recovered concentration of sodium fluorescein was found in the follicular infundibula, where it was still detectable after 48 h. Altogether, the results of this investigation revealed that differential stripping is a new method that can be used to study the penetration of topically applied substances into the follicular infundibula non-invasively and selectively. |
| doi_str_mv | 10.1111/j.0022-202X.2005.23779.x |
| format | Article |
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Recently, follicular penetration has become a major focus of interest. To date, a direct, non-invasive quantification of the amount of topically applied substance penetrated into the follicles had not been possible. The development of such a method was the aim of this study. Therefore, the advantages of both stripping techniques, tape stripping and cyanoacrylate skin surface biopsy, were combined and evaluated. Tape stripping was used to remove the part of the stratum corneum that contained the topically applied dye. Subsequently, the follicular contents were ripped off by cyanoacrylate skin surface biopsy. The combined method termed “differential stripping” was evaluated in vitro and in vivo, and the amount of topically applied fluorescent dye penetrated into the hair follicles was quantified after different penetration times. After 30 min, 5% of the recovered concentration of sodium fluorescein was found in the follicular infundibula, where it was still detectable after 48 h. Altogether, the results of this investigation revealed that differential stripping is a new method that can be used to study the penetration of topically applied substances into the follicular infundibula non-invasively and selectively.</description><identifier>ISSN: 0022-202X</identifier><identifier>ISSN: 0884-8734</identifier><identifier>EISSN: 1523-1747</identifier><identifier>EISSN: 1525-1497</identifier><identifier>DOI: 10.1111/j.0022-202X.2005.23779.x</identifier><identifier>PMID: 16098036</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Inc</publisher><subject>Administration, Topical ; Adult ; Animals ; Biological and medical sciences ; Biopsy - methods ; Contrast Media - pharmacokinetics ; cyanoacrylate skin surface biopsy ; Cyanoacrylates - pharmacokinetics ; Dermatology ; Female ; Fluorescein - pharmacokinetics ; follicular penetration ; follicular reservoir ; Hair Follicle - cytology ; Hair Follicle - metabolism ; Humans ; Male ; Medical sciences ; Skin Absorption ; Sus scrofa ; tape stripping</subject><ispartof>Journal of general internal medicine : JGIM, 2005-08, Vol.125 (2), p.264-269</ispartof><rights>2005 The Society for Investigative Dermatology, Inc</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Aug 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5569-4a58b6e54f96734f58447327530cb647d4c8ccefad7e6de860da2d6fd69a05943</citedby><cites>FETCH-LOGICAL-c5569-4a58b6e54f96734f58447327530cb647d4c8ccefad7e6de860da2d6fd69a05943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.0022-202X.2005.23779.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/210371750?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580,64390,64392,64394,72474</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17015515$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16098036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teichmann, Alexa</creatorcontrib><creatorcontrib>Jacobi, Ute</creatorcontrib><creatorcontrib>Ossadnik, Michael</creatorcontrib><creatorcontrib>Richter, Heike</creatorcontrib><creatorcontrib>Koch, Stefan</creatorcontrib><creatorcontrib>Sterry, Wolfram</creatorcontrib><creatorcontrib>Lademann, Jürgen</creatorcontrib><title>Differential Stripping: Determination of the Amount of Topically Applied Substances Penetrated into the Hair Follicles</title><title>Journal of general internal medicine : JGIM</title><addtitle>J Invest Dermatol</addtitle><description>The determination of penetration pathways of topically applied substances into the skin is the subject of several investigations. 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Recently, follicular penetration has become a major focus of interest. To date, a direct, non-invasive quantification of the amount of topically applied substance penetrated into the follicles had not been possible. The development of such a method was the aim of this study. Therefore, the advantages of both stripping techniques, tape stripping and cyanoacrylate skin surface biopsy, were combined and evaluated. Tape stripping was used to remove the part of the stratum corneum that contained the topically applied dye. Subsequently, the follicular contents were ripped off by cyanoacrylate skin surface biopsy. The combined method termed “differential stripping” was evaluated in vitro and in vivo, and the amount of topically applied fluorescent dye penetrated into the hair follicles was quantified after different penetration times. After 30 min, 5% of the recovered concentration of sodium fluorescein was found in the follicular infundibula, where it was still detectable after 48 h. Altogether, the results of this investigation revealed that differential stripping is a new method that can be used to study the penetration of topically applied substances into the follicular infundibula non-invasively and selectively.</abstract><cop>Oxford, UK</cop><pub>Elsevier Inc</pub><pmid>16098036</pmid><doi>10.1111/j.0022-202X.2005.23779.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Administration, Topical Adult Animals Biological and medical sciences Biopsy - methods Contrast Media - pharmacokinetics cyanoacrylate skin surface biopsy Cyanoacrylates - pharmacokinetics Dermatology Female Fluorescein - pharmacokinetics follicular penetration follicular reservoir Hair Follicle - cytology Hair Follicle - metabolism Humans Male Medical sciences Skin Absorption Sus scrofa tape stripping |
| title | Differential Stripping: Determination of the Amount of Topically Applied Substances Penetrated into the Hair Follicles |
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