Usnea barbata extract prevents ultraviolet-B induced prostaglandin E2 synthesis and COX-2 expression in HaCaT keratinocytes
Usnea barbata and its major constituent usnic acid are potent antimicrobial agents. Here, we have investigated anti-inflammatory properties of an U. barbata extract (UBE) containing 4% usnic acid in an ultraviolet-B (UVB) model with HaCaT keratinocytes. UVB irradiation induced PGE(2) production and...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2007-11, Vol.89 (1), p.9-14 |
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| container_title | Journal of photochemistry and photobiology. B, Biology |
| container_volume | 89 |
| creator | Engel, K Schmidt, U Reuter, J Weckesser, S Simon-Haarhaus, B Schempp, C M |
| description | Usnea barbata and its major constituent usnic acid are potent antimicrobial agents. Here, we have investigated anti-inflammatory properties of an U. barbata extract (UBE) containing 4% usnic acid in an ultraviolet-B (UVB) model with HaCaT keratinocytes. UVB irradiation induced PGE(2) production and COX-2 expression in a time and dose-dependent manner. UBE inhibited PGE(2) production at a half-maximal concentration of 60 microg/ml (2.4 microg/ml usnic acid) that did not affect the UVB-induced upregulation of COX-2, suggesting an effect on enzyme activity rather than on protein expression. The inhibition of PGE(2) production by UBE was not due to cytotoxicity. Besides its known antimicrobial properties, UBE displays specific UVB protective effects that might be useful in the topical treatment of UVB-mediated inflammatory skin conditions. |
| doi_str_mv | 10.1016/j.jphotobiol.2007.08.002 |
| format | Article |
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Here, we have investigated anti-inflammatory properties of an U. barbata extract (UBE) containing 4% usnic acid in an ultraviolet-B (UVB) model with HaCaT keratinocytes. UVB irradiation induced PGE(2) production and COX-2 expression in a time and dose-dependent manner. UBE inhibited PGE(2) production at a half-maximal concentration of 60 microg/ml (2.4 microg/ml usnic acid) that did not affect the UVB-induced upregulation of COX-2, suggesting an effect on enzyme activity rather than on protein expression. The inhibition of PGE(2) production by UBE was not due to cytotoxicity. Besides its known antimicrobial properties, UBE displays specific UVB protective effects that might be useful in the topical treatment of UVB-mediated inflammatory skin conditions.</description><identifier>ISSN: 1011-1344</identifier><identifier>DOI: 10.1016/j.jphotobiol.2007.08.002</identifier><identifier>PMID: 17766140</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Cell Extracts - pharmacology ; Cells, Cultured ; Cyclooxygenase 2 - biosynthesis ; Cyclooxygenase 2 - metabolism ; Dinoprostone - biosynthesis ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Enzymologic - radiation effects ; Keratinocytes - drug effects ; Keratinocytes - enzymology ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; Ultraviolet Rays ; Up-Regulation - drug effects ; Up-Regulation - radiation effects ; Usnea - chemistry</subject><ispartof>Journal of photochemistry and photobiology. 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B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>Usnea barbata and its major constituent usnic acid are potent antimicrobial agents. Here, we have investigated anti-inflammatory properties of an U. barbata extract (UBE) containing 4% usnic acid in an ultraviolet-B (UVB) model with HaCaT keratinocytes. UVB irradiation induced PGE(2) production and COX-2 expression in a time and dose-dependent manner. UBE inhibited PGE(2) production at a half-maximal concentration of 60 microg/ml (2.4 microg/ml usnic acid) that did not affect the UVB-induced upregulation of COX-2, suggesting an effect on enzyme activity rather than on protein expression. The inhibition of PGE(2) production by UBE was not due to cytotoxicity. Besides its known antimicrobial properties, UBE displays specific UVB protective effects that might be useful in the topical treatment of UVB-mediated inflammatory skin conditions.</description><subject>Cell Extracts - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Dinoprostone - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Enzymologic - radiation effects</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - enzymology</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - radiation effects</subject><subject>Ultraviolet Rays</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - radiation effects</subject><subject>Usnea - chemistry</subject><issn>1011-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEFPwzAMhXMAsTH4Cygnbi1xkybtEarBkCbtskncqrRNWUaXljpFTPx5IjF8sfSe_enZhFBgMTCQD4f4MOx731e27-KEMRWzLGYsuSDz4EMEXIgZuUY8sFCpVFdkBkpJCYLNyc8OndG00mOlvabm24-69nQYzZdxHunUBeEroI2Pnqh1zVSbJtg9ev3eaddYR5cJxZPze4MWaZBosXmLksAKFETbu7BHV7rQW_phRu2t6-uTN3hDLlvdobk99wXZPS-3xSpab15ei8d1NEAifKSg4m3LodUyzyUXkGeQJ0aoXKeSqVowwbOqTnKespSDkDIVTbCbcG4OTPEFuf_jhtifk0FfHi3WpgvxTT9hKTOhuAIIg3fnwak6mqYcRnvU46n8fxf_BdFsbf0</recordid><startdate>20071112</startdate><enddate>20071112</enddate><creator>Engel, K</creator><creator>Schmidt, U</creator><creator>Reuter, J</creator><creator>Weckesser, S</creator><creator>Simon-Haarhaus, B</creator><creator>Schempp, C M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20071112</creationdate><title>Usnea barbata extract prevents ultraviolet-B induced prostaglandin E2 synthesis and COX-2 expression in HaCaT keratinocytes</title><author>Engel, K ; Schmidt, U ; Reuter, J ; Weckesser, S ; Simon-Haarhaus, B ; Schempp, C M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-71b3ff31fa699634198192e479a5607c40438bc2935053146654de47d00091073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Cell Extracts - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Dinoprostone - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Enzymologic - radiation effects</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - enzymology</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - radiation effects</topic><topic>Ultraviolet Rays</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - radiation effects</topic><topic>Usnea - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Engel, K</creatorcontrib><creatorcontrib>Schmidt, U</creatorcontrib><creatorcontrib>Reuter, J</creatorcontrib><creatorcontrib>Weckesser, S</creatorcontrib><creatorcontrib>Simon-Haarhaus, B</creatorcontrib><creatorcontrib>Schempp, C M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Engel, K</au><au>Schmidt, U</au><au>Reuter, J</au><au>Weckesser, S</au><au>Simon-Haarhaus, B</au><au>Schempp, C M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Usnea barbata extract prevents ultraviolet-B induced prostaglandin E2 synthesis and COX-2 expression in HaCaT keratinocytes</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2007-11-12</date><risdate>2007</risdate><volume>89</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>1011-1344</issn><abstract>Usnea barbata and its major constituent usnic acid are potent antimicrobial agents. Here, we have investigated anti-inflammatory properties of an U. barbata extract (UBE) containing 4% usnic acid in an ultraviolet-B (UVB) model with HaCaT keratinocytes. 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| ispartof | Journal of photochemistry and photobiology. B, Biology, 2007-11, Vol.89 (1), p.9-14 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Cell Extracts - pharmacology Cells, Cultured Cyclooxygenase 2 - biosynthesis Cyclooxygenase 2 - metabolism Dinoprostone - biosynthesis Dose-Response Relationship, Drug Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Enzymologic - radiation effects Keratinocytes - drug effects Keratinocytes - enzymology Keratinocytes - metabolism Keratinocytes - radiation effects Ultraviolet Rays Up-Regulation - drug effects Up-Regulation - radiation effects Usnea - chemistry |
| title | Usnea barbata extract prevents ultraviolet-B induced prostaglandin E2 synthesis and COX-2 expression in HaCaT keratinocytes |
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