Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV),...
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| Veröffentlicht in: | Medical microbiology and immunology 2005-08, Vol.194 (4), p.181-185 |
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| creator | KIMURA, Hiroshi IHIRA, Masaru ENOMOTO, Yoshihiro KAWADA, Jun-Ichi ITO, Yoshinori MORISHIMA, Tsuneo YOSHIKAWA, Tetsushi ASANO, Yoshizo |
| description | Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system. |
| doi_str_mv | 10.1007/s00430-005-0242-9 |
| format | Article |
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To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.</description><identifier>ISSN: 0300-8584</identifier><identifier>EISSN: 1432-1831</identifier><identifier>DOI: 10.1007/s00430-005-0242-9</identifier><identifier>PMID: 15909202</identifier><identifier>CODEN: MMIYAO</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Acids ; Adolescent ; Adult ; Aged ; Biological and medical sciences ; Central Nervous System Viral Diseases - cerebrospinal fluid ; Central Nervous System Viral Diseases - diagnosis ; Child ; Deoxyribonucleic acid ; DNA ; DNA, Viral - cerebrospinal fluid ; Female ; Fundamental and applied biological sciences. Psychology ; Herpes Simplex - cerebrospinal fluid ; Herpes Simplex - diagnosis ; Herpes simplex virus ; Herpes viruses ; Humans ; Infant ; Infant, Newborn ; Male ; Microbiology ; Middle Aged ; Miscellaneous ; Nucleic Acid Amplification Techniques ; Polymerase Chain Reaction ; Predictive Value of Tests ; Research methodology ; Sensitivity and Specificity ; Simplexvirus - genetics ; Simplexvirus - isolation & purification ; Virology</subject><ispartof>Medical microbiology and immunology, 2005-08, Vol.194 (4), p.181-185</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-7b3d38b024a4bef01daa33d3b3ec8584cbaa5a35fc7230a09dc2aedaf020b18f3</citedby><cites>FETCH-LOGICAL-c453t-7b3d38b024a4bef01daa33d3b3ec8584cbaa5a35fc7230a09dc2aedaf020b18f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17035778$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15909202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIMURA, Hiroshi</creatorcontrib><creatorcontrib>IHIRA, Masaru</creatorcontrib><creatorcontrib>ENOMOTO, Yoshihiro</creatorcontrib><creatorcontrib>KAWADA, Jun-Ichi</creatorcontrib><creatorcontrib>ITO, Yoshinori</creatorcontrib><creatorcontrib>MORISHIMA, Tsuneo</creatorcontrib><creatorcontrib>YOSHIKAWA, Tetsushi</creatorcontrib><creatorcontrib>ASANO, Yoshizo</creatorcontrib><title>Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR</title><title>Medical microbiology and immunology</title><addtitle>Med Microbiol Immunol</addtitle><description>Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.</description><subject>Acids</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System Viral Diseases - cerebrospinal fluid</subject><subject>Central Nervous System Viral Diseases - diagnosis</subject><subject>Child</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - cerebrospinal fluid</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpes Simplex - cerebrospinal fluid</subject><subject>Herpes Simplex - diagnosis</subject><subject>Herpes simplex virus</subject><subject>Herpes viruses</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Polymerase Chain Reaction</subject><subject>Predictive Value of Tests</subject><subject>Research methodology</subject><subject>Sensitivity and Specificity</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - isolation & purification</subject><subject>Virology</subject><issn>0300-8584</issn><issn>1432-1831</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUuLFTEQhYMoznX0B7iRIOguWkn6kXY3XJ8wqAy6DtXpCmZIP0y6R_0X_mRzvRcG3LgKVL5zijqHsccSXkiA9mUGqDQIgFqAqpTo7rCdrLQS0mh5l-1AAwhTm-qMPcj5GkC2jYL77EzWHXQK1I79vsIlDHygldwa5onPnn-jtFDmOYxLpJ_8JqQt89cfL3iYuKNEfZrzEiaM3MetiF_x_TwumEIu-p7WH0QTj_O8iJGGgCsNvHytxXYsGiyuwQeHf9fhNPBEGMUaRuKf91cP2T2PMdOj03vOvr5982X_Xlx-evdhf3EpXFXrVbS9HrTpy9lY9eRBDoi6jHpN7nCx6xFr1LV3rdKA0A1OIQ3oQUEvjdfn7PnRd0nz943yaseQHcWIE81bto2pWmmM-S-oJFQdKF3Ap_-A1_OWSkyFUWCarqmbAskj5EqIOZG3Swojpl9Wgj2Uao-l2lKqPZRqu6J5cjLe-pLoreLUYgGenQDMDqNPOLmQb7kWdN22Rv8BLEWrww</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>KIMURA, Hiroshi</creator><creator>IHIRA, Masaru</creator><creator>ENOMOTO, Yoshihiro</creator><creator>KAWADA, Jun-Ichi</creator><creator>ITO, Yoshinori</creator><creator>MORISHIMA, Tsuneo</creator><creator>YOSHIKAWA, Tetsushi</creator><creator>ASANO, Yoshizo</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR</title><author>KIMURA, Hiroshi ; IHIRA, Masaru ; ENOMOTO, Yoshihiro ; KAWADA, Jun-Ichi ; ITO, Yoshinori ; MORISHIMA, Tsuneo ; YOSHIKAWA, Tetsushi ; ASANO, Yoshizo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-7b3d38b024a4bef01daa33d3b3ec8584cbaa5a35fc7230a09dc2aedaf020b18f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acids</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Central Nervous System Viral Diseases - cerebrospinal fluid</topic><topic>Central Nervous System Viral Diseases - diagnosis</topic><topic>Child</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - cerebrospinal fluid</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpes Simplex - cerebrospinal fluid</topic><topic>Herpes Simplex - diagnosis</topic><topic>Herpes simplex virus</topic><topic>Herpes viruses</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Polymerase Chain Reaction</topic><topic>Predictive Value of Tests</topic><topic>Research methodology</topic><topic>Sensitivity and Specificity</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - isolation & purification</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIMURA, Hiroshi</creatorcontrib><creatorcontrib>IHIRA, Masaru</creatorcontrib><creatorcontrib>ENOMOTO, Yoshihiro</creatorcontrib><creatorcontrib>KAWADA, Jun-Ichi</creatorcontrib><creatorcontrib>ITO, Yoshinori</creatorcontrib><creatorcontrib>MORISHIMA, Tsuneo</creatorcontrib><creatorcontrib>YOSHIKAWA, Tetsushi</creatorcontrib><creatorcontrib>ASANO, Yoshizo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Medical microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIMURA, Hiroshi</au><au>IHIRA, Masaru</au><au>ENOMOTO, Yoshihiro</au><au>KAWADA, Jun-Ichi</au><au>ITO, Yoshinori</au><au>MORISHIMA, Tsuneo</au><au>YOSHIKAWA, Tetsushi</au><au>ASANO, Yoshizo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR</atitle><jtitle>Medical microbiology and immunology</jtitle><addtitle>Med Microbiol Immunol</addtitle><date>2005-08-01</date><risdate>2005</risdate><volume>194</volume><issue>4</issue><spage>181</spage><epage>185</epage><pages>181-185</pages><issn>0300-8584</issn><eissn>1432-1831</eissn><coden>MMIYAO</coden><abstract>Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>15909202</pmid><doi>10.1007/s00430-005-0242-9</doi><tpages>5</tpages></addata></record> |
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| ispartof | Medical microbiology and immunology, 2005-08, Vol.194 (4), p.181-185 |
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| subjects | Acids Adolescent Adult Aged Biological and medical sciences Central Nervous System Viral Diseases - cerebrospinal fluid Central Nervous System Viral Diseases - diagnosis Child Deoxyribonucleic acid DNA DNA, Viral - cerebrospinal fluid Female Fundamental and applied biological sciences. Psychology Herpes Simplex - cerebrospinal fluid Herpes Simplex - diagnosis Herpes simplex virus Herpes viruses Humans Infant Infant, Newborn Male Microbiology Middle Aged Miscellaneous Nucleic Acid Amplification Techniques Polymerase Chain Reaction Predictive Value of Tests Research methodology Sensitivity and Specificity Simplexvirus - genetics Simplexvirus - isolation & purification Virology |
| title | Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR |
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