A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma
A novel strategy consisting of cleavable Isotope‐Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in ‘abnormal’ patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT...
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| Veröffentlicht in: | Proteomics (Weinheim) 2005-08, Vol.5 (12), p.3040-3044 |
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| container_issue | 12 |
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| container_title | Proteomics (Weinheim) |
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| creator | Leung, Kit-Yi Lescuyer, Pierre Campbell, James Byers, Helen L. Allard, Laure Sanchez, Jean-Charles Ward, Malcolm A. |
| description | A novel strategy consisting of cleavable Isotope‐Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in ‘abnormal’ patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT‐labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers. |
| doi_str_mv | 10.1002/pmic.200402101 |
| format | Article |
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These preliminary results show the promise of our approach to identify potential biomarkers.</description><subject>Algorithms</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Blood Proteins - chemistry</subject><subject>Cleavable isotope-coded affinity tag</subject><subject>Comparative proteomics</subject><subject>Computational Biology - methods</subject><subject>Databases, Protein</subject><subject>Humans</subject><subject>Immunoglobulin G - chemistry</subject><subject>Ions</subject><subject>Isotopes</subject><subject>MACCOT Distiller</subject><subject>Mass Spectrometry - methods</subject><subject>Peptide Mapping</subject><subject>Peptides - chemistry</subject><subject>Plasma</subject><subject>Proteins - chemistry</subject><subject>Proteome</subject><subject>Proteomics - methods</subject><subject>Software</subject><subject>Time Factors</subject><subject>Up-Regulation</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqVw5Yh84pbFzocdH1dpu1Tatkgt4mhNnPFicOI0dgr5BfxtUu1q4dbTzEjP-2qkJ0neM7pilGafhs7qVUZpQTNG2YvklHFWprLi7OVxL_OT5E0IPyhlopLidXLCOC0YF-Vp8mdNev-IjoQ4QsTdTKZg-x25Xt_Vt_fk3IZoncORGD8S6MHNwQbiDdEO4REah8QGH_2AqfYttgSMsb2NM4mwIy1EINGThwn6aM1MhtFHtD3R36HfYSDLOjgIHbxNXhlwAd8d5lny9fLivv6cbm83V_V6m-qCZSytcoFtQwGlZq2h0ApR8azQWcMbIaHSpaywYQ03mVluZLnkrcwybLXJOZj8LPm4710-eZgwRNXZoNE56NFPQfGq4JIV7FmQiZKXeSkXcLUH9ehDGNGoYbQdjLNiVD05Uk-O1NHREvhwaJ6aDtt_-EHKAsg98Ms6nJ-pU1-ur-r_y9N9dhGHv49ZGH8qLnJRqm83GyUvN-dbcXOn6vwva7iveg</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Leung, Kit-Yi</creator><creator>Lescuyer, Pierre</creator><creator>Campbell, James</creator><creator>Byers, Helen L.</creator><creator>Allard, Laure</creator><creator>Sanchez, Jean-Charles</creator><creator>Ward, Malcolm A.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma</title><author>Leung, Kit-Yi ; 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| fulltext | fulltext |
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| ispartof | Proteomics (Weinheim), 2005-08, Vol.5 (12), p.3040-3044 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Algorithms Animals Biomarkers Blood Proteins - chemistry Cleavable isotope-coded affinity tag Comparative proteomics Computational Biology - methods Databases, Protein Humans Immunoglobulin G - chemistry Ions Isotopes MACCOT Distiller Mass Spectrometry - methods Peptide Mapping Peptides - chemistry Plasma Proteins - chemistry Proteome Proteomics - methods Software Time Factors Up-Regulation |
| title | A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma |
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