Uncultivated Tannerella BU045 and BU063 are slim segmented filamentous rods of high prevalence but low abundance in inflammatory disease-associated dental plaques
Institute of Oral Biology, Section of Oral Microbiology and General Immunology, University of Zürich, Plattenstrasse 11, CH-8032 Zürich, Switzerland Correspondence Rudolf Gmür rudolf.gmuer{at}zzmk.uzh.ch Uncultivated clones BU045 and BU063 and Tannerella forsythia, a consensus periodontal pathogen...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2007-11, Vol.153 (11), p.3809-3816 |
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| creator | Zuger, Janine Luthi-Schaller, Helga Gmur, Rudolf |
| description | Institute of Oral Biology, Section of Oral Microbiology and General Immunology, University of Zürich, Plattenstrasse 11, CH-8032 Zürich, Switzerland
Correspondence Rudolf Gmür rudolf.gmuer{at}zzmk.uzh.ch
Uncultivated clones BU045 and BU063 and Tannerella forsythia, a consensus periodontal pathogen, are the closest known relatives within the genus Tannerella. They have been described to inhabit different ecological niches of the human oral cavity. In this study, fluorescent in situ hybridization (FISH) and immunofluorescence were combined to investigate the prevalence and abundance of BU045 and BU063 in comparison to T. forsythia in plaques from gingivitis, necrotizing ulcerative gingivitis (NUG) and chronic periodontitis. Phylotype-specific FISH probes identified BU045 and BU063 as elongated thin rods with a segmented structure. Two structurally similar and previously unknown, rare phylotypes (127 + and 997 + ) were also identified due to partial 16S rRNA sequence identity with T. forsythia . In gingivitis, NUG and periodontitis patients, BU045, BU063, 127 + , 997 + and T. forsythia were detected with prevalences of 50/83/71/14 and 81 %, 100/100/86/17 and 53 %, and 100/100/12/0 and 100 %, respectively. Supragingivally, colonization density of all five organisms was generally low, rarely exceeding 0.1 % of the total biota. In periodontal pocket samples, however, cell numbers of T. forsythia, but not of the uncultivable phylotypes, were greatly elevated. Our data demonstrate that Tannerella phylotypes BU045, BU063, 127 + and 997 + consist of long slim rods with segments, which, with respect to FISH stainability, often behaved as independent units. The phylotypes are frequent but low-level colonizers of various periodontal disease-associated plaques. Their apparent inability to proliferate to high density seems to exclude any relevance for the pathogenesis of periodontal diseases.
Abbreviations: FISH, fluorescent in situ hybridization; IF, indirect immunofluorescence; NUG, necrotizing ulcerative gingivitis |
| doi_str_mv | 10.1099/mic.0.2007/010926-0 |
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Correspondence Rudolf Gmür rudolf.gmuer{at}zzmk.uzh.ch
Uncultivated clones BU045 and BU063 and Tannerella forsythia, a consensus periodontal pathogen, are the closest known relatives within the genus Tannerella. They have been described to inhabit different ecological niches of the human oral cavity. In this study, fluorescent in situ hybridization (FISH) and immunofluorescence were combined to investigate the prevalence and abundance of BU045 and BU063 in comparison to T. forsythia in plaques from gingivitis, necrotizing ulcerative gingivitis (NUG) and chronic periodontitis. Phylotype-specific FISH probes identified BU045 and BU063 as elongated thin rods with a segmented structure. Two structurally similar and previously unknown, rare phylotypes (127 + and 997 + ) were also identified due to partial 16S rRNA sequence identity with T. forsythia . In gingivitis, NUG and periodontitis patients, BU045, BU063, 127 + , 997 + and T. forsythia were detected with prevalences of 50/83/71/14 and 81 %, 100/100/86/17 and 53 %, and 100/100/12/0 and 100 %, respectively. Supragingivally, colonization density of all five organisms was generally low, rarely exceeding 0.1 % of the total biota. In periodontal pocket samples, however, cell numbers of T. forsythia, but not of the uncultivable phylotypes, were greatly elevated. Our data demonstrate that Tannerella phylotypes BU045, BU063, 127 + and 997 + consist of long slim rods with segments, which, with respect to FISH stainability, often behaved as independent units. The phylotypes are frequent but low-level colonizers of various periodontal disease-associated plaques. Their apparent inability to proliferate to high density seems to exclude any relevance for the pathogenesis of periodontal diseases.
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Correspondence Rudolf Gmür rudolf.gmuer{at}zzmk.uzh.ch
Uncultivated clones BU045 and BU063 and Tannerella forsythia, a consensus periodontal pathogen, are the closest known relatives within the genus Tannerella. They have been described to inhabit different ecological niches of the human oral cavity. In this study, fluorescent in situ hybridization (FISH) and immunofluorescence were combined to investigate the prevalence and abundance of BU045 and BU063 in comparison to T. forsythia in plaques from gingivitis, necrotizing ulcerative gingivitis (NUG) and chronic periodontitis. Phylotype-specific FISH probes identified BU045 and BU063 as elongated thin rods with a segmented structure. Two structurally similar and previously unknown, rare phylotypes (127 + and 997 + ) were also identified due to partial 16S rRNA sequence identity with T. forsythia . In gingivitis, NUG and periodontitis patients, BU045, BU063, 127 + , 997 + and T. forsythia were detected with prevalences of 50/83/71/14 and 81 %, 100/100/86/17 and 53 %, and 100/100/12/0 and 100 %, respectively. Supragingivally, colonization density of all five organisms was generally low, rarely exceeding 0.1 % of the total biota. In periodontal pocket samples, however, cell numbers of T. forsythia, but not of the uncultivable phylotypes, were greatly elevated. Our data demonstrate that Tannerella phylotypes BU045, BU063, 127 + and 997 + consist of long slim rods with segments, which, with respect to FISH stainability, often behaved as independent units. The phylotypes are frequent but low-level colonizers of various periodontal disease-associated plaques. Their apparent inability to proliferate to high density seems to exclude any relevance for the pathogenesis of periodontal diseases.
Abbreviations: FISH, fluorescent in situ hybridization; IF, indirect immunofluorescence; NUG, necrotizing ulcerative gingivitis</description><subject>Aged</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Bacteroides forsythus</subject><subject>Bacteroidetes - classification</subject><subject>Bacteroidetes - genetics</subject><subject>Bacteroidetes - growth & development</subject><subject>Bacteroidetes - ultrastructure</subject><subject>Biological and medical sciences</subject><subject>Dental Plaque - microbiology</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gingivitis - microbiology</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Male</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Periodontitis - microbiology</subject><subject>Prevalence</subject><subject>RNA, Ribosomal, 16S - genetics</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9q3DAQxk1padK0T1AourSHgjcjS7LlYxv6DwK9bM5iLI0TFdnaSnZCXqdPWrm7kGNBMMPoNzPS91XVWw47Dn1_OXm7g10D0F1CKTRtDc-qcy5bVTeg4XnJhYIadNecVa9y_gVQLoG_rM5413cKejiv_tzMdg2Lv8eFHNvjPFOiEJB9vgGpGM5uy1rBMBHLwU8s0-1E80aPPuCWxjWzFF1mcWR3_vaOHRLdY6DZEhvWhYX4wHBYZ4dbxc_ljKVzwiWmR-Z8JsxUY87R-n_PcGUoBnYI-Hul_Lp6MWLI9OYUL6r91y_7q-_19c9vP64-XddWSrnUHe9E16J0vLfg-sHpUWnLh85RCWJUQnOhB2yc4hphoHZwZFE5dFIqLi6qD8exhxS3tYuZfLabFjOVH5pWF_Ea8X-wiC-l1rKA4gjaFHNONJpD8hOmR8PBbBaWRmvAbBaao4UGSte70_h1mMg99Zw8K8D7E4DZYhhTkdXnJ64XXQONKtzHI7d58uATmVuay8YUBx-31VwJw7kRGnrxF-87tgQ</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Zuger, Janine</creator><creator>Luthi-Schaller, Helga</creator><creator>Gmur, Rudolf</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Uncultivated Tannerella BU045 and BU063 are slim segmented filamentous rods of high prevalence but low abundance in inflammatory disease-associated dental plaques</title><author>Zuger, Janine ; Luthi-Schaller, Helga ; Gmur, Rudolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-717376a4d19c0d9bd8f58c1b7de8c13f538138ba2d518a0be6bdeca5dad44513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aged</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Bacteroides forsythus</topic><topic>Bacteroidetes - classification</topic><topic>Bacteroidetes - genetics</topic><topic>Bacteroidetes - growth & development</topic><topic>Bacteroidetes - ultrastructure</topic><topic>Biological and medical sciences</topic><topic>Dental Plaque - microbiology</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - analysis</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gingivitis - microbiology</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Male</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Periodontitis - microbiology</topic><topic>Prevalence</topic><topic>RNA, Ribosomal, 16S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zuger, Janine</creatorcontrib><creatorcontrib>Luthi-Schaller, Helga</creatorcontrib><creatorcontrib>Gmur, Rudolf</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zuger, Janine</au><au>Luthi-Schaller, Helga</au><au>Gmur, Rudolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uncultivated Tannerella BU045 and BU063 are slim segmented filamentous rods of high prevalence but low abundance in inflammatory disease-associated dental plaques</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>153</volume><issue>11</issue><spage>3809</spage><epage>3816</epage><pages>3809-3816</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Institute of Oral Biology, Section of Oral Microbiology and General Immunology, University of Zürich, Plattenstrasse 11, CH-8032 Zürich, Switzerland
Correspondence Rudolf Gmür rudolf.gmuer{at}zzmk.uzh.ch
Uncultivated clones BU045 and BU063 and Tannerella forsythia, a consensus periodontal pathogen, are the closest known relatives within the genus Tannerella. They have been described to inhabit different ecological niches of the human oral cavity. In this study, fluorescent in situ hybridization (FISH) and immunofluorescence were combined to investigate the prevalence and abundance of BU045 and BU063 in comparison to T. forsythia in plaques from gingivitis, necrotizing ulcerative gingivitis (NUG) and chronic periodontitis. Phylotype-specific FISH probes identified BU045 and BU063 as elongated thin rods with a segmented structure. Two structurally similar and previously unknown, rare phylotypes (127 + and 997 + ) were also identified due to partial 16S rRNA sequence identity with T. forsythia . In gingivitis, NUG and periodontitis patients, BU045, BU063, 127 + , 997 + and T. forsythia were detected with prevalences of 50/83/71/14 and 81 %, 100/100/86/17 and 53 %, and 100/100/12/0 and 100 %, respectively. Supragingivally, colonization density of all five organisms was generally low, rarely exceeding 0.1 % of the total biota. In periodontal pocket samples, however, cell numbers of T. forsythia, but not of the uncultivable phylotypes, were greatly elevated. Our data demonstrate that Tannerella phylotypes BU045, BU063, 127 + and 997 + consist of long slim rods with segments, which, with respect to FISH stainability, often behaved as independent units. The phylotypes are frequent but low-level colonizers of various periodontal disease-associated plaques. Their apparent inability to proliferate to high density seems to exclude any relevance for the pathogenesis of periodontal diseases.
Abbreviations: FISH, fluorescent in situ hybridization; IF, indirect immunofluorescence; NUG, necrotizing ulcerative gingivitis</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17975090</pmid><doi>10.1099/mic.0.2007/010926-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Bacteriological methods and techniques used in bacteriology Bacteriology Bacteroides forsythus Bacteroidetes - classification Bacteroidetes - genetics Bacteroidetes - growth & development Bacteroidetes - ultrastructure Biological and medical sciences Dental Plaque - microbiology DNA Probes DNA, Bacterial - analysis Female Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gingivitis - microbiology Humans In Situ Hybridization, Fluorescence Male Microbiology Middle Aged Periodontitis - microbiology Prevalence RNA, Ribosomal, 16S - genetics |
| title | Uncultivated Tannerella BU045 and BU063 are slim segmented filamentous rods of high prevalence but low abundance in inflammatory disease-associated dental plaques |
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