Mass Tagging Approach for Mitochondrial Thiol Proteins

A mass tagging approach is described for mitochondrial thiol proteins under nondenaturing conditions. This approach utilizes stable isotope-coded, thiol-reactive (4-iodobutyl)triphenylphosphonium (IBTP) reagents, i.e., the isotopomers IBTP-d0 and IBTP-d15. The mass spectrometric properties of IBTP-l...

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Veröffentlicht in:	Journal of proteome research 2005-07, Vol.4 (4), p.1403-1412
Hauptverfasser:	Marley, Kevin, Mooney, Duane T, Clark-Scannell, Gretchen, Tong, Tony T.-H, Watson, Jeffrey, Hagen, Tory M, Stevens, Jan F, Maier, Claudia S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Lactoglobulins - chemistry Lactoglobulins - genetics Mass Spectrometry - methods Mitochondria, Heart - chemistry Mitochondrial Proteins - chemistry Mitochondrial Proteins - genetics Models, Molecular Molecular Sequence Data Molecular Structure Molecular Weight Organophosphorus Compounds - chemistry Oxidation-Reduction Peptides - chemistry Peptides - genetics Rats Reproducibility of Results Sulfhydryl Compounds - chemistry Thioredoxins - chemistry Thioredoxins - genetics
Online-Zugang:	Volltext
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